Lake water samples were collected at specific depths with a five-liter Niskin bottle during normal LTER limnological sampling. Sub-samples were decanted into three-1 L Nalgene bottles (2-light and 1-amber), two-500 mL amber Nalgene bottles, three-150 mL borosilicate glass bottles, two-20 mL scintillation vials, and one-30 mL serum vial. A 500 mL sample from the one liter amber Nalgene bottle was decanted and filtered through a combusted Whatman 25 mm GF/F filter (475 degrees C for 4 hours). The filter was placed onto an aluminum weighing pan and air dried. Once the filters were dry, the pans were stacked together and frozen for later analysis.
Filters were acidified with HCL fumes, and analyzed using an Elemental Analyzer (currently a CE Elantech Flash EA 1112 Elemental Analyzer). Total particulate organic carbon (POC) and particulate organic nitrogen (PON) was obtained by comparison with an acetanilide standard curve.
Prior to the 0304 season, analysis was done on a 10 mm diameter sub-sample punch of the GF/F filter; from 0304 to present, the entire GF/F filter was analyzed.