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Lake water samples were collected at specific depths with a five-liter Niskin bottle during normal LTER limnological sampling. Sub-samples were decanted into three-1 L Nalgene bottles (2-light and 1-amber), two-500 mL amber Nalgene bottles, three-150 mL borosilicate glass bottles, two-20 mL scintillation vials, and one-30 mL serum vial. A 500 mL sample from the one liter amber Nalgene bottle was decanted and filtered through a combusted Whatman 25 mm GF/F filter (475 degrees C for 4 hours). After the sample was filtered, the filter tower was rinsed with 20 mL of deionized water. The filter was placed onto an aluminum weighing pan and air dried. Once the filters were dry, the pans were stacked together and frozen for later analysis in McMurdo by ASA Analytical services. A filter paper punch was used to take a sub-sample (10 mm diameter) from the Whatman 25 mm GF/F filter and was analyzed using a Carlo-Erba Elemental Analyzer. The elemental analyzer was standardized with acetanilide. Total particulate organic carbon (POC) and particulate organic nitrogen (PON was obtained by dividing the total area of the seston on the filter by the punch area.