Transplant Lake Integrated Connectivity Experiment (tLICE) in Taylor Valley, Antarctica, January 2018

Summary

Abstract: 

The purpose of this experiment, performed as part of the McMurdo Dry Valleys Long Term Ecological Research (MCM LTER) program, was to investigate the impact of lake level rise and moat expansion on microbial community diversity and function in the East Lobe of Lake Bonney, located in Taylor Valley, Antarctica. The “tLICE” experiment tested the following MCM5 Hypotheses: H3-Disturbance increases connectivity and accelerates shifts towards homogeneity, and H4-Decreased heterogeneity reduces community resistance and resilience. Lake water from the East Lobe of Lake Bonney was collected at depths representing maximum phytoplankton productivity (5 m, 17 m, 23 m), and transferred to dialysis bags. The bags were then attached to PVC frames and transplanted to new locations within the lake in two experiments: 1) a moat transplant, in which 5 m communities were transplanted to the open water moat; and 2) shallow to deep transplant, in which communities from all three sampling depths were moved 3 m down the water column to mimic approximately one decade of lake level rise. Transplanted samples were incubated under these conditions for two weeks, and then frames were recovered and samples were processed for a number of measurements. This data package includes the following measurements: nutrient concentration, chlorophyll-a content, chlorophyll fluorescence, cell density, amplicon gene sequences (16S and 18S rRNA genes), and physical parameters.

Date Range: 

January 13, 2018 to January 28, 2018
Data Citation
Morgan-kiss, R. 2020. McMurdo Dry Valleys LTER: Transplant Lake Integrated Connectivity Experiment (tLICE) in Taylor Valley, Antarctica, January 2018. Environmental Data Initiative. DOI: 10.6073/pasta/00cbcc20ab63feb5bb69a9109f35b697. Dataset accessed 31 May 2020.
Dataset(s)

Description: 

tLICE measurements

Variables (click to expand): 

DATASET_CODE
  • Label: Dataset code
  • Definition: Internal code
  • Type: Nominal
  • Missing values: None specified
COLLECTION_DATE
  • Label: Sample collection date.
  • Definition: Date sample was collected for analyses.
  • Type: Date/time
  • Date format: MM/DD/YYYY
  • Missing values: None specified
LOCATION
  • Label: Location
  • Definition: Lake where sample was collected.
  • Type: Nominal
  • Missing values: None specified
SAMPLE_TYPE
  • Label: Sample type
  • Definition: Was the sample from the water column or the moat?
  • Type: Nominal
  • Missing values: None specified
TREATMENT
  • Label: Treatment
  • Definition: Was the sample a control or transplant?
  • Type: Nominal
  • Missing values: None specified
REPLICATE
  • Label: Sample replicate
  • Definition: Sample replicate - either A, B, or C.
  • Type: Nominal
  • Missing values: None specified
TIMEPOINT
  • Label: Timepoint
  • Definition: T0 = timepoint 0, or the beginning of the experiment for the respective sample. T1 = timepoint 1, or the end of the experiment for the respective sample.
  • Type: Nominal
  • Missing values: None specified
DEPTH_M
  • Label: Depth
  • Definition: Depth in the water column.
  • Type: Physical quantity
  • Unit: meter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
SRP_MM
  • Label: Soluble reactive phosphorus
  • Definition: Phosphate molar concentration.
  • Type: Physical quantity
  • Unit: milliM
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
NO2_MM
  • Label: Nitrite concentration
  • Definition: Nitrite molar concentration.
  • Type: Physical quantity
  • Unit: milliM
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
NO3_MM
  • Label: Nitrate concentration
  • Definition: Nitrate molar concentration.
  • Type: Physical quantity
  • Unit: milliM
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
NH4_MM
  • Label: Ammonium concentration
  • Definition: Ammonium molar concentration.
  • Type: Physical quantity
  • Unit: milliM
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
CHLA_UG_L
  • Label: Chlorophyll-a concentration.
  • Definition: Chlorophyll-a concentration, as averaged from two replicates.
  • Type: Physical quantity
  • Unit: microgramsPerLiter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
CHLA_BL_UG_L
  • Label: Chlorophyll-a fluorescence (blue-green algae)
  • Definition: Chlorophyll-a fluorescence of the blue-green algae.
  • Type: Physical quantity
  • Unit: microgramsPerLiter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
CHLA_GR_UG_L
  • Label: Chlorophyll-a fluorescence (green algae)
  • Definition: Chlorophyll-a fluorescence of the green algae.
  • Type: Physical quantity
  • Unit: microgramsPerLiter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
CHLA_BR_UG_L
  • Label: Chlorophyll-a fluorescence (brown algae)
  • Definition: Chlorophyll-a fluorescence of the brown algae.
  • Type: Physical quantity
  • Unit: microgramsPerLiter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
CHLA_PE_UG_L
  • Label: Chlorophyll-a fluorescence (phycoerythrin algae)
  • Definition: Chlorophyll-a fluorescence of the phycoerythrin algae.
  • Type: Physical quantity
  • Unit: microgramsPerLiter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
CHLA_TOTAL_UG_L
  • Label: Total chlorophyll-a fluorescence.
  • Definition: Total chlorophyll-a fluorescence.
  • Type: Physical quantity
  • Unit: microgramsPerLiter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
FV_FM_BL
  • Label: Photochemical efficiency (blue-green algae)
  • Definition: Photochemical efficiency (Fv/Fm) of the blue-green algae.
  • Type: Nominal
  • Missing values: None specified
FV_FM_GR
  • Label: Photochemical efficiency (green algae)
  • Definition: Photochemical efficiency (Fv/Fm) of the green algae.
  • Type: Nominal
  • Missing values: None specified
FV_FM_BR
  • Label: Photochemical efficiency (brown algae)
  • Definition: Photochemical efficiency (Fv/Fm) of the brown algae.
  • Type: Nominal
  • Missing values: None specified
FV_FM_PE
  • Label: Photochemical efficiency (phycoerythrin algae)
  • Definition: Photochemical efficiency (Fv/Fm) of the phycoerythrin algae.
  • Type: Nominal
  • Missing values: None specified
YII_BL
  • Label: Photosystem II photochemical yield (blue-green algae)
  • Definition: Photosystem II photochemical yield of the blue-green algae.
  • Type: Nominal
  • Missing values: None specified
YII_GR
  • Label: Photosystem II photochemical yield (green algae)
  • Definition: Photosystem II photochemical yield of the green algae.
  • Type: Nominal
  • Missing values: None specified
YII_BR
  • Label: Photosystem II photochemical yield (brown algae)
  • Definition: Photosystem II photochemical yield of the brown algae.
  • Type: Nominal
  • Missing values: None specified
YII_PE
  • Label: Photosystem II photochemical yield (phycoerythrin algae)
  • Definition: Photosystem II photochemical yield of the phycoerythrin algae.
  • Type: Nominal
  • Missing values: None specified
NPQ_BL
  • Label: Non-photochemical quenching coefficient (blue-green algae)
  • Definition: Non-photochemical quenching coefficient for the blue-green algae.
  • Type: Nominal
  • Missing values: None specified
NPQ_GR
  • Label: Non-photochemical quenching coefficient (green algae)
  • Definition: Non-photochemical quenching coefficient for the green algae.
  • Type: Nominal
  • Missing values: None specified
NPQ_BR
  • Label: Non-photochemical quenching coefficient (brown algae)
  • Definition: Non-photochemical quenching coefficient for the brown algae.
  • Type: Nominal
  • Missing values: None specified
NPQ_PE
  • Label: Non-photochemical quenching coefficient (phycoerythrin algae)
  • Definition: Non-photochemical quenching coefficient for the phycoerythrin algae.
  • Type: Nominal
  • Missing values: None specified
NO_BL
  • Label: Non-regulated photochemical quenching coefficient (blue-green algae)
  • Definition: Non-regulated photochemical quenching coefficient for the blue-green algae.
  • Type: Nominal
  • Missing values: None specified
NO_GR
  • Label: Non-regulated photochemical quenching coefficient (green algae)
  • Definition: Non-regulated photochemical quenching coefficient for the green algae.
  • Type: Nominal
  • Missing values: None specified
NO_BR
  • Label: Non-regulated photochemical quenching coefficient (brown algae)
  • Definition: Non-regulated photochemical quenching coefficient for the brown algae.
  • Type: Nominal
  • Missing values: None specified
NO_PE
  • Label: Non-regulated photochemical quenching coefficient (phycoerythrin algae)
  • Definition: Non-regulated photochemical quenching coefficient for the phycoerythrin algae.
  • Type: Nominal
  • Missing values: None specified
PHOTOTROPHS_CELLS_ML
  • Label: Phototroph cell density
  • Definition: Phototroph cell density.
  • Type: Physical quantity
  • Unit: cellsPerMilliliter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
HETEROTROPHS_LARGE_CELLS_ML
  • Label: Heterotroph cell density, where cell size >1 um
  • Definition: Heterotroph cell density, where cell size >1 um.
  • Type: Physical quantity
  • Unit: cellsPerMilliliter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
HETEROTROPHS_SMALL_CELLS_ML
  • Label: Heterotroph cell density, where cell size <1 um
  • Definition: Heterotroph cell density, where cell size <1 um.
  • Type: Physical quantity
  • Unit: cellsPerMilliliter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
ACCN_16S
  • Label: Accession number (16S rRNA genes)
  • Definition: NCBI accession number for associated 16S rRNA gene raw sequence reads.
  • Type: Nominal
  • Missing values: None specified
ACCN_18S
  • Label: Accession number (18S rRNA genes)
  • Definition: NCBI accession number for associated 18S rRNA gene raw sequence reads.
  • Type: Nominal
  • Missing values: None specified
COMMENTS
  • Label: Comments
  • Definition: Comments.
  • Type: Nominal
  • Missing values: None specified

File: 

...or query the dataset by variables and dates of interest:

Description: 

Physical parameters (i.e., conductivity, temperature, PAR)

Variables (click to expand): 

DATASET_CODE
  • Label: Dataset code
  • Definition: Internal code.
  • Type: Nominal
  • Missing values: None specified
Collection Date
  • Label: Sample collection date.
  • Definition: Date sample was collected.
  • Type: Date/time
  • Date format: MM/DD/YYYY
  • Missing values: None specified
LOCATION
  • Label: Location
  • Definition: Lake where sample was collected.
  • Type: Nominal
  • Missing values: None specified
SAMPLE_TYPE
  • Label: Sample type
  • Definition: Was the sample from the water column or the moat?
  • Type: Nominal
  • Missing values: None specified
DEPTH_M
  • Label: Depth
  • Definition: Depth in the water column.
  • Type: Physical quantity
  • Unit: meter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
CONDUCTIVITY_MS_CM
  • Label: Conductivity
  • Definition: Conductivity
  • Type: Physical quantity
  • Unit: millisiemensPerCentimeter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
TEMPERATURE_C
  • Label: Temperature
  • Definition: Water temperature
  • Type: Physical quantity
  • Unit: celsius
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
PAR_UMOL_M2_S
  • Label: PAR
  • Definition: Photosynthetically active radiation
  • Type: Physical quantity
  • Unit: micromolePerMeterSquaredPerSecond
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
COMMENTS
  • Label: Comments
  • Definition: Comments
  • Type: Nominal
  • Missing values: None specified

File: 

...or query the dataset by variables and dates of interest:

Short name: 

LIMNO_TLICE

Dataset ID: 

3004
People

Principal Investigator(s): 

Contact: 

Associated Personnel: 

Associated Researcher
Lab Technician
Data Manager