02619nas a2200205   4500008004100000245006000041210006000101260006000161300000800221490001000229520198900239653001302228653002702241653001302268653001702281653001002298100002402308700003302332856004802365       2024                        eng d00aMetagenomic analysis of Antarctic microbial communities0 aMetagenomic analysis of Antarctic microbial communities  aAlbuquerque, NM, USAbUniversity of New Mexicoc06/2024  a1360 vPh.D.3 a<p>The research presented in this dissertation focuses on microbial roles in biogeochemical cycling in Antarctic aquatic environments. The major objective of my research was to examine the impact of biotic and abiotic pressures on nutrient cycling in microbially dominated systems. I used three perennially ice covered lakes in the McMurdo Dry Valleys (MDVs) along with subglacial lakes Whillans and Mercer as natural case studies. The MDVs are located in Victoria Land, East Antarctica and have been studied since 1993 as part of the McMurdo Long Term Ecological Research Project. Viruses in the MDVs were shown to be seasonally abundant and have high infection rates. My work built upon this previous research by showing that viruses are also diverse and potentially re-direct host metabolism through auxiliary metabolic genes (AMGs). Subglacial lake Whillans and Mercer lie underneath 800 and 1100 meters of ice respectively beneath the West Antarctic Ice Sheet. Previous studies in subglacial lakes Whillans have shown an active prokaryotic community. Through viral-like particle counts, microscopy, and metagenomics, we established the presence and potential metabolic influence of viruses in both Whillans and Mercer. In MDV lakes have an extensive high-throughput DNA dataset studying microbial communities through the 16S rRNA gene. We used metagenomic sequencing within two MCM lakes at multiple depths to build upon these previous datasets by annotating metagenomically assembled genomes for functional characteristics. This project highlights the lakes&#39; vertical redox gradient, showing that dominant taxa, nutrient cycling genes, and metabolic potentials change down the water column. The MDVs serve as a biological indicator for climate change in continental Antarctica and functional changes serve as important indicators of climate change. Taken together, this dissertation shows the importance of multi-kingdom microbial function under oligotrophic conditions.</p>10abacteria10abiogeochemical cycling10afunction10ametagenomics10avirus1 aRobinson, David, M.1 aTakacs-Vesbach, Cristina, D.  uhttps://www.proquest.com/docview/3119910585