02372nas a2200193   4500008004100000245016100041210006900202260001200271490000700283520165600290653002101946653002601967653002701993653001702020653002902037100001202066700002902078856007102107       2019                        eng d00aInfluence of environmental drivers and potential interactions on the distribution of microbial communities from three permanently stratified Antarctic lakes0 aInfluence of environmental drivers and potential interactions on  c05/20190 v103 a<p>The McMurdo Dry Valley (MDV) lakes represent unique habitats in the microbial world. Perennial ice covers protect liquid water columns from either significant allochthonous inputs or seasonal mixing, resulting in centuries of stable biogeochemistry. Extreme environmental conditions including low seasonal photosynthetically active radiation (PAR), near freezing temperatures, and oligotrophy have precluded higher trophic levels from the food webs. Despite these limitations, diverse microbial life flourishes in the stratified water columns, including Archaea, bacteria, fungi, protists, and viruses. While a few recent studies have applied next generation sequencing, a thorough understanding of the MDV lake microbial diversity and community structure is currently lacking. Here we used Illumina MiSeq sequencing of the 16S and 18S rRNA genes combined with a microscopic survey of key eukaryotes to compare the community structure and potential interactions among the bacterial and eukaryal communities within the water columns of Lakes Bonney (east and west lobes, ELB, and WLB, respectively) and Fryxell (FRX). Communities were distinct between the upper, oxic layers and the dark, anoxic waters, particularly among the bacterial communities residing in WLB and FRX. Both eukaryal and bacterial community structure was influenced by different biogeochemical parameters in the oxic and anoxic zones. Bacteria formed complex interaction networks which were lake-specific. Several eukaryotes exhibit potential interactions with bacteria in ELB and WLB, while interactions between these groups in the more productive FRX were relatively rare.</p>10aAquatic protists10aenvironmental drivers10aheterotrophic bacteria10ainteractions10aMcMurdo Dry Valley lakes1 aLi, Wei1 aMorgan-Kiss, Rachael, M.  uhttps://www.frontiersin.org/articles/10.3389/fmicb.2019.01067/full