<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Cook, Greg</style></author><author><style face="normal" font="default" size="100%">Teufel, Amber</style></author><author><style face="normal" font="default" size="100%">Kalra, Isha</style></author><author><style face="normal" font="default" size="100%">Li, Wei</style></author><author><style face="normal" font="default" size="100%">Wang, Xin</style></author><author><style face="normal" font="default" size="100%">John C. Priscu</style></author><author><style face="normal" font="default" size="100%">Rachael M. Morgan-Kiss</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">The Antarctic psychrophiles &lt;i&gt;Chlamydomonas&lt;/i&gt; spp. UWO241 and ICE-MDV exhibit differential restructuring of photosystem I in response to iron</style></title><secondary-title><style face="normal" font="default" size="100%">Photosynthesis Research</style></secondary-title><short-title><style face="normal" font="default" size="100%">Photosynth Res</style></short-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antarctica</style></keyword><keyword><style  face="normal" font="default" size="100%">Cyclic electron flow</style></keyword><keyword><style  face="normal" font="default" size="100%">Iron</style></keyword><keyword><style  face="normal" font="default" size="100%">Photosystem I</style></keyword><keyword><style  face="normal" font="default" size="100%">Psychrophile</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">02/2019</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://link.springer.com/article/10.1007/s11120-019-00621-0</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">9</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;em&gt;Chlamydomonas &lt;/em&gt;sp. UWO241 is a psychrophilic alga isolated from the deep photic zone of a perennially ice-covered Antarctic lake (east lobe Lake Bonney, ELB). Past studies have shown that &lt;em&gt;C&lt;/em&gt;. sp. UWO241 exhibits constitutive downregulation of photosystem I (PSI) and high rates of PSI-associated cyclic electron flow (CEF). Iron levels in ELB are in the nanomolar range leading us to hypothesize that the unusual PSI phenotype of &lt;em&gt;C&lt;/em&gt;. sp. UWO241 could be a response to chronic Fe-deficiency. We studied the impact of Fe availability in &lt;em&gt;C&lt;/em&gt;. sp. UWO241, a mesophile, C. &lt;em&gt;reinhardtii&lt;/em&gt; SAG11-32c, as well as a psychrophile isolated from the shallow photic zone of ELB, &lt;em&gt;Chlamydomonas&lt;/em&gt; sp. ICE-MDV. Under Fe-deficiency, PsaA abundance and levels of photooxidizable P700 (ΔA&lt;sub&gt;820&lt;/sub&gt;/A&lt;sub&gt;820&lt;/sub&gt;) were reduced in both psychrophiles relative to the mesophile. Upon increasing Fe, &lt;em&gt;C&lt;/em&gt;. sp. ICE-MDV and C. &lt;em&gt;reinhardtii&lt;/em&gt; exhibited restoration of PSI function, while &lt;em&gt;C&lt;/em&gt;. sp. UWO241 exhibited only moderate changes in PSI activity and lacked almost all LHCI proteins. Relative to Fe-excess conditions (200 μM Fe&lt;sup&gt;2+&lt;/sup&gt;), &lt;em&gt;C&lt;/em&gt;. sp. UWO241 grown in 18 μM Fe&lt;sup&gt;2+&lt;/sup&gt; exhibited downregulation of light harvesting and photosystem core proteins, as well as upregulation of a bestrophin-like anion channel protein and two CEF-associated proteins (NdsS, PGL1). Key enzymes of starch synthesis and shikimate biosynthesis were also upregulated. We conclude that in response to variable Fe availability, the psychrophile &lt;em&gt;C&lt;/em&gt;. sp. UWO241 exhibits physiological plasticity which includes restructuring of the photo-chemical apparatus, increased PSI-associated CEF, and shifts in downstream carbon metabolism toward storage carbon and secondary stress metabolites.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>32</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Cook, Greg</style></author><author><style face="normal" font="default" size="100%">Rachael M. Morgan-Kiss</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Antarctic &lt;i&gt;Chlamydomonas&lt;/i&gt; strains &lt;i&gt;C.&lt;/i&gt; sp. UWO241 and ICE-MDV exhibit differential restructuring of the photosynthetic apparatus in response to iron</style></title><secondary-title><style face="normal" font="default" size="100%">Department of Microbiology</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2018</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://rave.ohiolink.edu/etdc/view?acc_num=miami1525455621778836</style></url></web-urls></urls><publisher><style face="normal" font="default" size="100%">Miami University</style></publisher><pub-location><style face="normal" font="default" size="100%">Oxford, OH</style></pub-location><volume><style face="normal" font="default" size="100%">M.S.</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;As an integral cofactor for many redox-associated processes, iron (Fe) homeostasis is crucial in order to produce sufficient energy for the organism. Fe limitation, or excess, can cause major alterations in the function and structure of the photosynthetic apparatus. Photosynthetic psychrophiles grown under permanent low temperatures exhibit novel adaptations in their photosynthetic apparatus to deal with this permanent stress. The ice-covered lakes of the McMurdo Dry Valleys harbor many species of cold-adapted algae, including &lt;em&gt;Chlamydomonas&lt;/em&gt; sp. UWO241 (UWO241). As a consequence of adaptation to multiple permanent extreme conditions, UWO241 exhibits a remodeled photosynthetic apparatus for maintaining redox poise. One unusual characteristic of UWO241 is the absence of a PSI-associated 77K fluorescence emission under a wide range of growth conditions. This phenotype resembles Fe deficiency in other model organisms such as &lt;em&gt;C.&lt;/em&gt; reinhardtii. We hypothesized that adaptation to permanent iron deficiency in its native environment may contribute to this unusual phenotype. We compared the effect of Fe availability on the physiology and photobiology of UWO241 with the model &lt;em&gt;C.&lt;/em&gt; reinhardtii as well as a second psychrophilic alga, &lt;em&gt;Chlamydomonas&lt;/em&gt; sp. ICE-MDV (ICE). The impacts of a restructured photosynthetic apparatus on the unique Fe-associated phenotype in UWO241 will be discussed.&lt;/p&gt;</style></abstract><work-type><style face="normal" font="default" size="100%">masters</style></work-type></record></records></xml>