<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kalra, Isha</style></author><author><style face="normal" font="default" size="100%">Wang, Xin</style></author><author><style face="normal" font="default" size="100%">Zhang, Ru</style></author><author><style face="normal" font="default" size="100%">Rachael M. Morgan-Kiss</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">High salt-induced PSI-supercomplex is associated with high CEF and attenuation of state transitions</style></title><secondary-title><style face="normal" font="default" size="100%">Photosynthesis Research</style></secondary-title><short-title><style face="normal" font="default" size="100%">Photosynth Res</style></short-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">acclimation</style></keyword><keyword><style  face="normal" font="default" size="100%">Antarctica</style></keyword><keyword><style  face="normal" font="default" size="100%">Chlamydomonas</style></keyword><keyword><style  face="normal" font="default" size="100%">Cyclic electron flow</style></keyword><keyword><style  face="normal" font="default" size="100%">PSI-supercomplex</style></keyword><keyword><style  face="normal" font="default" size="100%">salinity</style></keyword><keyword><style  face="normal" font="default" size="100%">state transitions</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2023</style></year><pub-dates><date><style  face="normal" font="default" size="100%">09/2023</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://link.springer.com/10.1007/s11120-023-01032-y</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">157</style></volume><pages><style face="normal" font="default" size="100%">65 - 84</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;While PSI-driven cyclic electron flow (CEF) and assembly of thylakoid supercomplexes have been described in model organisms like &lt;i&gt;Chlamydomonas reinhardtii&lt;/i&gt;, open questions remain regarding their contributions to survival under long-term stress. The Antarctic halophyte, &lt;i&gt;C. priscuii UWO241&lt;/i&gt; (UWO241), possesses constitutive high CEF rates and a stable PSI-supercomplex as a consequence of adaptation to permanent low temperatures and high salinity. To understand whether CEF represents a broader acclimation strategy to short- and long-term stress, we compared high salt acclimation between the halotolerant UWO241, the salt-sensitive model, &lt;i&gt;C. reinhardtii&lt;/i&gt;, and a moderately halotolerant Antarctic green alga, &lt;i&gt;C.&lt;/i&gt; sp. ICE-MDV (ICE-MDV). CEF was activated under high salt and associated with increased non-photochemical quenching in all three &lt;i&gt;Chlamydomonas&lt;/i&gt; species. Furthermore, high salt-acclimated cells of either strain formed a PSI-supercomplex, while state transition capacity was attenuated. How the CEF-associated PSI-supercomplex interferes with state transition response is not yet known. We present a model for interaction between PSI-supercomplex formation, state transitions, and the important role of CEF for survival during long-term exposure to high salt.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kalra, Isha</style></author><author><style face="normal" font="default" size="100%">Wang, Xin</style></author><author><style face="normal" font="default" size="100%">Cvetkovska, Marina</style></author><author><style face="normal" font="default" size="100%">Jeong, Jooyeon</style></author><author><style face="normal" font="default" size="100%">McHargue, William</style></author><author><style face="normal" font="default" size="100%">Zhang, Ru</style></author><author><style face="normal" font="default" size="100%">Hüner, Norman</style></author><author><style face="normal" font="default" size="100%">Yuan, Joshua S.</style></author><author><style face="normal" font="default" size="100%">Rachael M. Morgan-Kiss</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Chlamydomonas sp. UWO 241 exhibits high cyclic electron flow and rewired metabolism under high salinity</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Physiology</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">03/2020</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.plantphysiol.org/content/early/2020/04/03/pp.19.01280</style></url></web-urls></urls><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The Antarctic green alga &lt;em&gt;Chlamydomonas&lt;/em&gt; sp. UWO 241 (UWO 241) is adapted to permanent low temperatures, hypersalinity, and extreme shade. one of the most striking phenotypes of UWO 241 is an altered photosystem I (PSI) organization and constitutive PSI cyclic electron flow (CEF). To date, little attention has been paid to CEF during long-term stress acclimation, and the consequences of sustained CEF in UWO 241 are not known. In this study, we combined photobiology, proteomics, and metabolomics to understand the underlying role of sustained CEF in high salinity stress acclimation. High salt-grown UWO 241 exhibited increased thylakoid proton motive flux and an increased capacity for non-photochemical quenching. Under high salt, a significant proportion of the upregulated enzymes were associated with the Calvin Benson Bassham Cycle, carbon storage metabolism, and protein translation. Two key enzymes of the Shikimate pathway, DAHP synthase and chorismate synthase, were also upregulated, as well as indole-3-glycerol phosphate synthase, an enzyme involved in the biosynthesis of L-tryptophan and indole acetic acid. In addition, several compatible solutes (glycerol, proline, and sucrose) accumulated to high levels in high salt-grown UWO 241 cultures. We suggest that UWO 241 maintains constitutively high CEF through the associated PSI-cytochrome b6f supercomplex to support robust growth and strong photosynthetic capacity under a constant growth regime of low temperatures and high salinity.&lt;/p&gt;</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Cook, Greg</style></author><author><style face="normal" font="default" size="100%">Teufel, Amber</style></author><author><style face="normal" font="default" size="100%">Kalra, Isha</style></author><author><style face="normal" font="default" size="100%">Li, Wei</style></author><author><style face="normal" font="default" size="100%">Wang, Xin</style></author><author><style face="normal" font="default" size="100%">John C. Priscu</style></author><author><style face="normal" font="default" size="100%">Rachael M. Morgan-Kiss</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">The Antarctic psychrophiles &lt;i&gt;Chlamydomonas&lt;/i&gt; spp. UWO241 and ICE-MDV exhibit differential restructuring of photosystem I in response to iron</style></title><secondary-title><style face="normal" font="default" size="100%">Photosynthesis Research</style></secondary-title><short-title><style face="normal" font="default" size="100%">Photosynth Res</style></short-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antarctica</style></keyword><keyword><style  face="normal" font="default" size="100%">Cyclic electron flow</style></keyword><keyword><style  face="normal" font="default" size="100%">Iron</style></keyword><keyword><style  face="normal" font="default" size="100%">Photosystem I</style></keyword><keyword><style  face="normal" font="default" size="100%">Psychrophile</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">02/2019</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://link.springer.com/article/10.1007/s11120-019-00621-0</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">9</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;em&gt;Chlamydomonas &lt;/em&gt;sp. UWO241 is a psychrophilic alga isolated from the deep photic zone of a perennially ice-covered Antarctic lake (east lobe Lake Bonney, ELB). Past studies have shown that &lt;em&gt;C&lt;/em&gt;. sp. UWO241 exhibits constitutive downregulation of photosystem I (PSI) and high rates of PSI-associated cyclic electron flow (CEF). Iron levels in ELB are in the nanomolar range leading us to hypothesize that the unusual PSI phenotype of &lt;em&gt;C&lt;/em&gt;. sp. UWO241 could be a response to chronic Fe-deficiency. We studied the impact of Fe availability in &lt;em&gt;C&lt;/em&gt;. sp. UWO241, a mesophile, C. &lt;em&gt;reinhardtii&lt;/em&gt; SAG11-32c, as well as a psychrophile isolated from the shallow photic zone of ELB, &lt;em&gt;Chlamydomonas&lt;/em&gt; sp. ICE-MDV. Under Fe-deficiency, PsaA abundance and levels of photooxidizable P700 (ΔA&lt;sub&gt;820&lt;/sub&gt;/A&lt;sub&gt;820&lt;/sub&gt;) were reduced in both psychrophiles relative to the mesophile. Upon increasing Fe, &lt;em&gt;C&lt;/em&gt;. sp. ICE-MDV and C. &lt;em&gt;reinhardtii&lt;/em&gt; exhibited restoration of PSI function, while &lt;em&gt;C&lt;/em&gt;. sp. UWO241 exhibited only moderate changes in PSI activity and lacked almost all LHCI proteins. Relative to Fe-excess conditions (200 μM Fe&lt;sup&gt;2+&lt;/sup&gt;), &lt;em&gt;C&lt;/em&gt;. sp. UWO241 grown in 18 μM Fe&lt;sup&gt;2+&lt;/sup&gt; exhibited downregulation of light harvesting and photosystem core proteins, as well as upregulation of a bestrophin-like anion channel protein and two CEF-associated proteins (NdsS, PGL1). Key enzymes of starch synthesis and shikimate biosynthesis were also upregulated. We conclude that in response to variable Fe availability, the psychrophile &lt;em&gt;C&lt;/em&gt;. sp. UWO241 exhibits physiological plasticity which includes restructuring of the photo-chemical apparatus, increased PSI-associated CEF, and shifts in downstream carbon metabolism toward storage carbon and secondary stress metabolites.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue></record></records></xml>