<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Raymond, James A.</style></author><author><style face="normal" font="default" size="100%">Rachael M. Morgan-Kiss</style></author><author><style face="normal" font="default" size="100%">Stahl-Rommel, Sarah</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Glycerol is an osmoprotectant in two Antarctic &lt;I&gt;Chlamydomonas&lt;/I&gt; species from an ice-covered saline lake and is synthesized by an unusual bidomain enzyme</style></title><secondary-title><style face="normal" font="default" size="100%">Frontiers in Plant Science</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antarctica</style></keyword><keyword><style  face="normal" font="default" size="100%">Chlamydomonas</style></keyword><keyword><style  face="normal" font="default" size="100%">glycerol synthesis</style></keyword><keyword><style  face="normal" font="default" size="100%">glycerol-3-phosphate dehydrogenase</style></keyword><keyword><style  face="normal" font="default" size="100%">Lake Bonney</style></keyword><keyword><style  face="normal" font="default" size="100%">phosphoserine phosphatase</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">08/2020</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://www.frontiersin.org/articles/10.3389/fpls.2020.01259/full</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">11</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Glycerol, a compatible solute, has previously been found to act as an osmoprotectant in some marine &lt;em&gt;Chlamydomonas&lt;/em&gt; species and several species of &lt;em&gt;Dunaliella&lt;/em&gt; from hypersaline ponds. Recently, &lt;em&gt;Chlamydomonas reinhardtii&lt;/em&gt; and &lt;em&gt;Dunaliella salina&lt;/em&gt; were shown to make glycerol with an unusual bidomain enzyme, which appears to be unique to algae, that contains a phosphoserine phosphatase and glycerol-3-phosphate dehydrogenase. Here we report that two psychrophilic species of &lt;em&gt;Chlamydomonas&lt;/em&gt; (&lt;em&gt;C.&lt;/em&gt; spp. UWO241 and ICE-MDV) from Lake Bonney, Antarctica also produce high levels of glycerol to survive in the lake&amp;rsquo;s saline waters. Glycerol concentration increased linearly with salinity and at 1.3 M NaCl, exceeded 400 mM in &lt;em&gt;C.&lt;/em&gt; sp. UWO241, the more salt-tolerant strain. We also show that both species expressed several isoforms of the bidomain enzyme. An analysis of one of the isoforms of &lt;em&gt;C.&lt;/em&gt; sp. UWO241 showed that it was strongly upregulated by NaCl and is thus the likely source of glycerol. These results reveal another adaptation of the Lake Bonney &lt;em&gt;Chlamydomonas&lt;/em&gt; species that allow them to survive in an extreme polar environment.&lt;/p&gt;</style></abstract></record></records></xml>