<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Dragone, Nicholas B.</style></author><author><style face="normal" font="default" size="100%">Childress, Mary K.</style></author><author><style face="normal" font="default" size="100%">Vanderburgh, Caihong</style></author><author><style face="normal" font="default" size="100%">Willmore, Rachel</style></author><author><style face="normal" font="default" size="100%">Hogg, Ian D.</style></author><author><style face="normal" font="default" size="100%">Sancho, Leopoldo G.</style></author><author><style face="normal" font="default" size="100%">Charles K. Lee</style></author><author><style face="normal" font="default" size="100%">John E. Barrett</style></author><author><style face="normal" font="default" size="100%">Quandt, C. Alisha</style></author><author><style face="normal" font="default" size="100%">LeMonte, Joshua J.</style></author><author><style face="normal" font="default" size="100%">Adams, Byron J.</style></author><author><style face="normal" font="default" size="100%">Noah Fierer</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A comprehensive survey of soil microbial diversity across the Antarctic continent</style></title><secondary-title><style face="normal" font="default" size="100%">Polar Biology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antarctica</style></keyword><keyword><style  face="normal" font="default" size="100%">bacteria</style></keyword><keyword><style  face="normal" font="default" size="100%">fungi</style></keyword><keyword><style  face="normal" font="default" size="100%">microbial ecology</style></keyword><keyword><style  face="normal" font="default" size="100%">soils</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2025</style></year><pub-dates><date><style  face="normal" font="default" size="100%">02/2025</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://link.springer.com/10.1007/s00300-025-03372-y</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">48</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Antarctic soils are unique from those found nearly anywhere else on Earth yet can still harbor a broad diversity of microorganisms able to tolerate the challenging conditions typical of the continent. For these reasons, microbiologists have been drawn to Antarctica for decades. However, our understanding of which microbes thrive in Antarctic soils and how they to do so remains limited. To help resolve these knowledge gaps, we analyzed a collection of 200 archived Antarctic soils&amp;mdash;from Livingston Island on the Antarctic Peninsula to Cape Hallett in northern Victoria Land. We analyzed the prokaryotic and fungal communities in these soils using both cultivation-independent marker gene sequencing and cultivation-dependent approaches (microbial isolation), paired with extensive soil geochemical analyses. Our cultivation-independent analyses indicate that colder, saltier, and drier soils harbor less diverse communities of bacteria and fungi, distinct from those found in soils with less challenging conditions. We also built a culture collection from a subset of these soils that encompasses more than 50 bacterial and fungal genera, including cold-tolerant organisms, such as &amp;lt;i&amp;gt;Cryobacterium&amp;lt;/i&amp;gt; and &amp;lt;i&amp;gt;Cryomyces&amp;lt;/i&amp;gt;. By directly comparing the diversity of our cultured isolates against our cultivation-independent data, we show that many of the more abundant Antarctic taxa are not readily cultivated and highlight bacterial and fungal taxa that should be the focus of future cultivation efforts. Together, we hope that our collection of isolates, the comprehensive data compiled from the cultivation-independent analyses, and our geochemical analyses will serve as a community resource to accelerate the study of Antarctic soil microbes.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>32</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Dragone, Nicholas B.</style></author></authors><secondary-authors><author><style face="normal" font="default" size="100%">Noah Fierer</style></author></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Microbial life in challenging environments</style></title><secondary-title><style face="normal" font="default" size="100%">Department of Ecology and Evolutionary Biology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antarctica</style></keyword><keyword><style  face="normal" font="default" size="100%">environmental conditions</style></keyword><keyword><style  face="normal" font="default" size="100%">microbial ecology</style></keyword><keyword><style  face="normal" font="default" size="100%">microorganisms</style></keyword><keyword><style  face="normal" font="default" size="100%">soils</style></keyword><keyword><style  face="normal" font="default" size="100%">tonga</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2023</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://www.proquest.com/docview/2814734209</style></url></web-urls></urls><publisher><style face="normal" font="default" size="100%">University of Colorado Boulder</style></publisher><pub-location><style face="normal" font="default" size="100%">Boulder, CO</style></pub-location><volume><style face="normal" font="default" size="100%">Ph.D.</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Microorganisms are nearly ubiquitous on Earth, but the identity and function of microbial communities are inherently dependent on the properties of the specific environment in question. Here, I have studied soils around the world to answer questions about how the functional attributes of microorganisms allow them to respond to challenging environmental conditions. First, I explore how microbial communities in soils change across environmental gradients in Antarctica. I show that microbes in Antarctic surface soils are most restricted by low temperatures, low water availability, and high concentrations of salt. Microbial communities near the polar plateau, the most challenging environment, are dominated by Actinobacteria and Chloroflexi, and are enriched in genes associated with the oxidation of hydrogen gas as an energy source. Second, I show that the earliest microbial colonizers of a newly-formed volcanic island in the Kingdom of Tonga are chemolithotrophs that appear to have come from nearby geothermal systems. While many of these microbes utilize sulfur as an energy source, the most abundant organisms have genes that indicate they can oxidize trace gases including carbon monoxide and hydrogen. Finally, I show that organisms associated with carbon limited subsurface soils tend to have smaller genomes, grow more slowly, and have more gene pathways associated with metabolism and the storage of carbon. Taken together, these studies shed light on microbial survival in challenging soil environments and show the varied ways in which microbial communities interact with and are affected by their surroundings.&lt;/p&gt;</style></abstract><work-type><style face="normal" font="default" size="100%">doctoral</style></work-type></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Dragone, Nicholas B.</style></author><author><style face="normal" font="default" size="100%">Henley, Jessica B.</style></author><author><style face="normal" font="default" size="100%">Holland-Moritz, Hannah</style></author><author><style face="normal" font="default" size="100%">Melisa A. Diaz</style></author><author><style face="normal" font="default" size="100%">Hogg, Ian D.</style></author><author><style face="normal" font="default" size="100%">W. Berry Lyons</style></author><author><style face="normal" font="default" size="100%">Diana H. Wall</style></author><author><style face="normal" font="default" size="100%">Byron Adams</style></author><author><style face="normal" font="default" size="100%">Noah Fierer</style></author></authors><secondary-authors><author><style face="normal" font="default" size="100%">Mackelprang, Rachel</style></author></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Elevational constraints on the composition and genomic attributes of microbial communities in Antarctic soils</style></title><secondary-title><style face="normal" font="default" size="100%">mSystems</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antarctica</style></keyword><keyword><style  face="normal" font="default" size="100%">microbial ecology</style></keyword><keyword><style  face="normal" font="default" size="100%">soil microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">soils</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">01/2022</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://journals.asm.org/doi/full/10.1128/msystems.01330-21</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">7</style></volume><pages><style face="normal" font="default" size="100%">e01330-21</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The inland soils found on the Antarctic continent represent one of the more challenging environments for microbial life on Earth. Nevertheless, Antarctic soils harbor unique bacterial and archaeal (prokaryotic) communities able to cope with extremely cold and dry conditions. These communities are not homogeneous, and the taxonomic composition and functional capabilities (genomic attributes) of these communities across environmental gradients remain largely undetermined. We analyzed the prokaryotic communities in soil samples collected from across the Shackleton Glacier region of Antarctica by coupling quantitative PCR, marker gene amplicon sequencing, and shotgun metagenomic sequencing. We found that elevation was the dominant factor explaining differences in the structures of the soil prokaryotic communities, with the drier and saltier soils found at higher elevations harboring less diverse communities and unique assemblages of cooccurring taxa. The higher-elevation soil communities also had lower maximum potential growth rates (as inferred from metagenome-based estimates of codon usage bias) and an overrepresentation of genes associated with trace gas metabolism. Together, these results highlight the utility of assessing community shifts across pronounced environmental gradients to improve our understanding of the microbial diversity found in Antarctic soils and the strategies used by soil microbes to persist at the limits of habitability.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue></record></records></xml>