<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Dragone, Nicholas B.</style></author><author><style face="normal" font="default" size="100%">Childress, Mary K.</style></author><author><style face="normal" font="default" size="100%">Vanderburgh, Caihong</style></author><author><style face="normal" font="default" size="100%">Willmore, Rachel</style></author><author><style face="normal" font="default" size="100%">Hogg, Ian D.</style></author><author><style face="normal" font="default" size="100%">Sancho, Leopoldo G.</style></author><author><style face="normal" font="default" size="100%">Charles K. Lee</style></author><author><style face="normal" font="default" size="100%">John E. Barrett</style></author><author><style face="normal" font="default" size="100%">Quandt, C. Alisha</style></author><author><style face="normal" font="default" size="100%">LeMonte, Joshua J.</style></author><author><style face="normal" font="default" size="100%">Adams, Byron J.</style></author><author><style face="normal" font="default" size="100%">Noah Fierer</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A comprehensive survey of soil microbial diversity across the Antarctic continent</style></title><secondary-title><style face="normal" font="default" size="100%">Polar Biology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antarctica</style></keyword><keyword><style  face="normal" font="default" size="100%">bacteria</style></keyword><keyword><style  face="normal" font="default" size="100%">fungi</style></keyword><keyword><style  face="normal" font="default" size="100%">microbial ecology</style></keyword><keyword><style  face="normal" font="default" size="100%">soils</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2025</style></year><pub-dates><date><style  face="normal" font="default" size="100%">02/2025</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://link.springer.com/10.1007/s00300-025-03372-y</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">48</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Antarctic soils are unique from those found nearly anywhere else on Earth yet can still harbor a broad diversity of microorganisms able to tolerate the challenging conditions typical of the continent. For these reasons, microbiologists have been drawn to Antarctica for decades. However, our understanding of which microbes thrive in Antarctic soils and how they to do so remains limited. To help resolve these knowledge gaps, we analyzed a collection of 200 archived Antarctic soils&amp;mdash;from Livingston Island on the Antarctic Peninsula to Cape Hallett in northern Victoria Land. We analyzed the prokaryotic and fungal communities in these soils using both cultivation-independent marker gene sequencing and cultivation-dependent approaches (microbial isolation), paired with extensive soil geochemical analyses. Our cultivation-independent analyses indicate that colder, saltier, and drier soils harbor less diverse communities of bacteria and fungi, distinct from those found in soils with less challenging conditions. We also built a culture collection from a subset of these soils that encompasses more than 50 bacterial and fungal genera, including cold-tolerant organisms, such as &amp;lt;i&amp;gt;Cryobacterium&amp;lt;/i&amp;gt; and &amp;lt;i&amp;gt;Cryomyces&amp;lt;/i&amp;gt;. By directly comparing the diversity of our cultured isolates against our cultivation-independent data, we show that many of the more abundant Antarctic taxa are not readily cultivated and highlight bacterial and fungal taxa that should be the focus of future cultivation efforts. Together, we hope that our collection of isolates, the comprehensive data compiled from the cultivation-independent analyses, and our geochemical analyses will serve as a community resource to accelerate the study of Antarctic soil microbes.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Dragone, Nicholas B.</style></author><author><style face="normal" font="default" size="100%">Melisa A. Diaz</style></author><author><style face="normal" font="default" size="100%">Hogg, Ian D.</style></author><author><style face="normal" font="default" size="100%">W. Berry Lyons</style></author><author><style face="normal" font="default" size="100%">W. Andrew Jackson</style></author><author><style face="normal" font="default" size="100%">Diana H. Wall</style></author><author><style face="normal" font="default" size="100%">Adams, Byron J.</style></author><author><style face="normal" font="default" size="100%">Noah Fierer</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Exploring the boundaries of microbial habitability in soil</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Geophysical Research: Biogeosciences</style></secondary-title><short-title><style face="normal" font="default" size="100%">J Geophys Res Biogeosci</style></short-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antarctica</style></keyword><keyword><style  face="normal" font="default" size="100%">astrobiology</style></keyword><keyword><style  face="normal" font="default" size="100%">bacteria</style></keyword><keyword><style  face="normal" font="default" size="100%">extremophiles</style></keyword><keyword><style  face="normal" font="default" size="100%">fungi</style></keyword><keyword><style  face="normal" font="default" size="100%">soils</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">06/2021</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://agupubs.onlinelibrary.wiley.com/doi/abs/10.1029/2020JG006052</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">126</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Microbes are widely assumed to be capable of colonizing even the most challenging terrestrial surface environments on Earth given enough time. We would not expect to find surface soils uninhabited by microbes as soils typically harbor diverse microbial communities and viable microbes have been detected in soils exposed to even the most inhospitable conditions. However, if uninhabited soils do exist, we might expect to find them in Antarctica. We analyzed 204 ice-free soils collected from across a remote valley in the Transantarctic Mountains (84&amp;ndash;85&amp;deg;S, 174&amp;ndash;177&amp;deg;W) and were able to identify a potential limit of microbial habitability. While most of the soils we tested contained diverse microbial communities, with fungi being particularly ubiquitous, microbes could not be detected in many of the driest, higher elevation soils&amp;mdash;results that were confirmed using cultivation-dependent, cultivation-independent, and metabolic assays. While we cannot confirm that this subset of soils is completely sterile and devoid of microbial life, our results suggest that microbial life is severely restricted in the coldest, driest, and saltiest Antarctic soils. Constant exposure to these conditions for thousands of years has limited microbial communities so that their presence and activity is below detectable limits using a variety of standard methods. Such soils are unlikely to be unique to the studied region with this work supporting previous hypotheses that microbial habitability is constrained by near-continuous exposure to cold, dry, and salty conditions, establishing the environmental conditions that limit microbial life in terrestrial surface soils.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue></record></records></xml>