Diana H. Wall 2014-10-31 tabular digitial data McMurdo Dry Valleys LTER McMurdo Dry Valleys LTER 10.6073/pasta/f278284d88a56c21c68662210c8aeb36 https://mcm.lternet.edu/content/stream-transects-anhydrobiosis-nematodes Investigation of the effect of hydrological and biogeochemical linkages in the transition zone between streams and surrounding soils on invertebrate community structure as part of the McMurdo Dry Valleys Long Term Ecological Research (LTER) project. The invertebrate community structure with relation to soil moisture, salinity, and chlorophyll-a was determined. The study took place on 28 November 1997 and 31 December 1997 at the Von Guerard Stream/Harnish Creek network. 1997-11-28 1997-12-31 ground condition  Metadata completed in 2016. This file was created by Pilar Tillberg on 11 May 2001, using raw data from the Excel workbook '9711rsan.raw'. The file format was suggested by the LTER data manager, to conform with the relational database structure. [PT 11 May 2001]. As needed Transect by Von Guerard Stream at Upper Site Moss Site M-05 Transect 5 Points T051 and T052 mark the start and end of the transect. Start : IDs: 5VGUPPERT051 Ellipsoid: 114.08 ORTHOMETRIC HT (m) 169.27 End: ID: 5VGUPPERT052 Ellipsoid: 120.24 ORTHOMETRIC HT (m) 175.41 File provenance: strm-clg.txt 163.307327270508 163.305282592773 -77.634208679199 -77.634559631348 172m 172m meter Transect by Von Guerard Stream at gage Moss Site M-12, F12Vong-G Transect 12 Points T121 and T122 mark the start and end of the transect. Start : IDs: 12VGGAGET121, Ellipsoid: -30.67 ORTHOMETRIC HT (m) 24.53 End: ID: 12VGGAGET122 Ellipsoid: -31.35 ORTHOMETRIC HT (m) 23.87 File provenance: strm-clg.txt 163.255294799805 163.253005981445 -77.609222412109 -77.609474182129 24m 24m meter USGS site 5; coordinates taken from 1996-97 GPS measurements at center of weir Parent Stream: Von Guerard Stream Provenance : GPS96-97.DOC ID: vguerard_f6 163.253997802734 163.253997802734 -77.608200073242 -77.608200073242 19m 19m meter Von Guerard Stream at F21. Id: uvg_f21.This was an ill-fated upstream gage on Von Guerard, 2.5 kilometers upstream from F6.  It was installed during the lowest season of record (00-01) and then promptly destroyed during the 01-02 season.  It's parts were stripped and it never generated a hydrology database, but was certainly sampled. 163.301254272461 163.301254272461 -77.626380920410 -77.626380920410 0m 0m meter On the Stream team data page 163.302963256836 163.302963256836 -77.632797241211 -77.632797241211 Parent Stream: Harnish Creek Tributary, also known as Relict Channel 163.294113159180 163.294113159180 -77.633735656738 -77.633735656738 130m 130m meter LTER Core Areas population dynamics None <cntorg>McMurdo Dry Valleys LTER</cntorg> <onlink>http://mcmlter.org/</onlink> <span property="dc:title" content="McMurdo Dry Valleys LTER" class="rdf-meta element-hidden"></span> Name: Ross A. Virginia Role: associated researcher Name: John "Jeb" E. Barrett Role: field crew Name: Byron J. Adams Role: field crew Name: Inigo San Gil Role: data manager Not Applicable Not Applicable Field and/or Lab Methods Approximately 1500g of soil or sediment were collected into Whirl-Pak(tm) bags with clean plastic scoops from 5 sections (0, 8, 16, 24, and 32 m away from the stream) along each transect. Five grams of sediment/soil were also collected from the top 1 cm for measurement of chlorophyll-a concentrations. Samples were mixed within the bags, placed into insulated coolers, and transported for analysis to the McMurdo laboratories. The soils were processed within 24 days of collection from the field. Until then, the soils were stored in a 4_C refrigerator. To extract the anhydrobiotic (coiled) nematodes, 100g of soil were placed into a beaker with 200mL 1.25M sugar solution. Then, a long spatula was used to stir the soil so that it was suspended in the sugar (30 seconds of stirring). Next, the sample was poured onto a set of No. 40 over No. 400 U.S.A. standard testing sieves that had been pre-wetted with 1.25M sugar solution. The top screen was rinsed with 1.25M sugar solution and then removed. The bottom screen was rinsed with 1.25M sugar solution that was squirted only on the front of the screen at an angle. Using this rinsing method, the soil was carefully worked to the bottom wedge of the screen, and the sugar volume was reduced by gently tapping the screen and letting more solution pass through. Next, the sample was rinsed into a 150mL beaker through a funnel, and then the funnel rinsed to catch any residue. Once the sample was collected into the 150mL beaker, the sugar and sediment was pipetted (using an automatic pipette) into a centrifuge tube containing 2M sugar solution. The pipetting was done slowly and at an angle so that the boundary between the two sugar layers was maintained. Then the beaker was rinsed with 1.25M sugar solution and the dregs were pipetted into the centrifuge tube as well. When necessary, more than one centrifuge tube was used to collect all the sample. Next, the tubes with sample were centrifuged for five minutes at 1744 RPM. Then, the liquid contents of the tube were poured through a No. 500 sieve. Finally, the contents of the sieve were rinsed into glass centrifuge tubes and stored at 4_C until counting. Samples were washed in to a counting dish and examined under a microscope at x100 or x200 magnification. Nematodes were identified as coiled or straight and counted. Total numbers in each sample were recorded on data sheets. Data were entered in to Excel files, printed, and checked for errors. Approximately 1500g of soil or sediment were collected into Whirl-Pak(tm) bags with clean plastic scoops from 5 sections (0, 8, 16, 24, and 32 m away from the stream) along each transect. Five grams of sediment/soil were also collected from the top 1 cm for measurement of chlorophyll-a concentrations. Samples were mixed within the bags, placed into insulated coolers, and transported for analysis to the McMurdo laboratories. The soils were processed within 24 days of collection from the field. Until then, the soils were stored in a 4_C refrigerator. To extract the anhydrobiotic (coiled) nematodes, 100g of soil were placed into a beaker with 200mL 1.25M sugar solution. Then, a long spatula was used to stir the soil so that it was suspended in the sugar (30 seconds of stirring). Next, the sample was poured onto a set of No. 40 over No. 400 U.S.A. standard testing sieves that had been pre-wetted with 1.25M sugar solution. The top screen was rinsed with 1.25M sugar solution and then removed. The bottom screen was rinsed with 1.25M sugar solution that was squirted only on the front of the screen at an angle. Using this rinsing method, the soil was carefully worked to the bottom wedge of the screen, and the sugar volume was reduced by gently tapping the screen and letting more solution pass through. Next, the sample was rinsed into a 150mL beaker through a funnel, and then the funnel rinsed to catch any residue. Once the sample was collected into the 150mL beaker, the sugar and sediment was pipetted (using an automatic pipette) into a centrifuge tube containing 2M sugar solution. The pipetting was done slowly and at an angle so that the boundary between the two sugar layers was maintained. Then the beaker was rinsed with 1.25M sugar solution and the dregs were pipetted into the centrifuge tube as well. When necessary, more than one centrifuge tube was used to collect all the sample. Next, the tubes with sample were centrifuged for five minutes at 1744 RPM. Then, the liquid contents of the tube were poured through a No. 500 sieve. Finally, the contents of the sieve were rinsed into glass centrifuge tubes and stored at 4_C until counting. Samples were washed in to a counting dish and examined under a microscope at x100 or x200 magnification. Nematodes were identified as coiled or straight and counted. Total numbers in each sample were recorded on data sheets. Data were entered in to Excel files, printed, and checked for errors. unknown rsan Stream Transects - Anhydrobiosis of Nematodes - Data spreadsheet description, units used and column descriptions LOCATION Name of area where measurement was made The data provider Name of area where measurement was made DATE_TIME Date on which sample was gathered The data provider calendar date/time MM/DD/YYYY h:m:s gregorian calendar SAMPLE # Sample ID The data provider Sample ID TYPE OF ORGANISM Family associated with organism The data provider Family associated with organism SPECIES Species found in soil The data provider Species found in soil SEX Gender of organism (male vs. female) The data provider Gender of organism (male vs. female) LIVE/DEAD/COMBINED Survival Status (living, dead, or both) The data provider Survival Status (living, dead, or both) ADULT/JUVENILE/COMBINED Maturity (adult, juvenile, or both) The data provider Maturity (adult, juvenile, or both) PERCENT COILED NEMATODES Percentage of the nematodes found in a coiled state The data provider 0.01 100 dimensionless Comments Comments The data provider Comments FILE NAME Name of file in which data was stored The data provider Name of file in which data was stored McMurdo Dry Valleys LTER The data distributor shall not be liable for innacuracies in the content http 1 0 \r 1 column , https://mcm.lternet.edu/sites/default/files/data/rsan.csv None 2014-10-31 2014-10-31 McMurdo Dry Valleys LTER http://mcmlter.org/ Biological Data Profile of the Content Standards for Digital Geospatial Metadata devised by the Federal Geographic Data Committee. 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