Johanna Laybourn-Parry 2014-11-04 tabular digitial data McMurdo Dry Valleys LTER McMurdo Dry Valleys LTER 10.6073/pasta/3cd7d9d45bd614fea2904e4f149ff5ae https://mcm.lternet.edu/content/microzooplankton-lake-fryxell-ciliates-bacteria-nanoflagellates In conjunction with the Long Term Ecological Research (LTER) project in the McMurdo Dry Valleys of Antarctica, lakes were monitored for microzooplankton by a team based out of the University of Nottingham (led by Johanna Laybourn-Parry). This dataset shows numbers of ciliates, bacteria, heterotrophic nanoflagellates, and phototrophic nanoflagellates found at various depths in Lake Fryxell. 1992-01-01 1994-01-31 ground condition Metadata completed in 2016.Data migrated to the Drupal Ecological Information Management System (DEIMS in 2014 (San Gil)Data for this file was submitted by Johanna Laybourn-Parry to the data manager at INSTAAR on October 21, 1998. Files were sent via e-mail as well as a hard copy. The original version of the file is stored on the Unix system in "/data1/data/lakes/plankton/laybourn-parry/Fryxell". Upon arrival at INSTAAR, the data manager reformatted the file to present it in a relational mode. This was done using Microsoft Access. It was then exported in comma delimited ascii and MS-DOS text format to present on the web. Links to these files are provided above. As needed The Lake Fryxell basin is formed by a moraine depression in a wider portion of the Taylor Valley. It has a number of moraine islands and shallower areas, as well as several relatively well developed deltas. The lake is fed by at least 10 meltwater streams with a total drainage catchment of 230 km2. The lake is dammed to the southwest by the Canada Glacier and is topographically closed. It is perennially ice covered; during summer months, an ice-free moat generally forms around much of the lake margin. Lake levels have risen ~2 m between 1971 and 1996. There are no surface outflows; the only known water loss is through ice ablation (evaporation, sublimation and physical scouring). Valley: Taylor Distance to Sea : 9 Maximum Length (km): 5.8 Maximum Width (km): 2.1 Maximum Depth (m): 20 Surface Area (km^2): 7.08 Ice Thickness Average Surface (m): 3.3 - 4.5 Volume (m^3 * 10^6): 25.2 163.259582519531 163.048782348633 -77.597076416016 -77.622711181641 18m 18m meter LTER Core Areas population dynamics None <cntorg>McMurdo Dry Valleys LTER</cntorg> <onlink>http://mcmlter.org/</onlink> <span property="dc:title" content="McMurdo Dry Valleys LTER" class="rdf-meta element-hidden"></span> Name: Inigo San Gil Role: data manager Not Applicable Not Applicable Field and/or Lab Methods Water was collected using either a Kemmerer bottle or a Van Dorn sampler lowered through a hole in the 4-m-thick ice with a Jiffy-drill in January 1992 and in January 1994 at a center station (depth 18.5 m). Samples were taken at 1-m intervals down to 12 m into the anoxic zone. In 1992, 250-ml subsamples were fixed in Lugol's iodine for the analysis of ciliates, and other aliquots fixed in glutaraldehyde for bacterial counts. In1994, 1.1-l samples were fixed in buffered glutaraldehyde for protozoological and bacterial analysis. These were kept refrigerated in the dark and flown to Australia or New Zealand for immediate analysis. Both fixatives are regarded as reliable for protistan analysis provided samples are analysed within a few months of collection. However, in our experience while Lugol's iodine is considered best for preserving abundance, it does cause more cell distortion of some species than glutaraldehyde, which gives better preservation of ciliates in freshwater samples. One liter of water from each depth was preserved in Lugol's iodine for phytoplankton counts. Temperature was measured with a (YSI) Yellow Springs Instruments 54 meter and probe, and conductivity with a Radiometer Conductivity meter. Ten milliliters of the buffered glutaraldehyde-fixed material was stained with DAPI, and filtered onto a 0.2-microm black polycarbonate filter, and bacterial numbers were counted under epifluorescence microscopy using UV excitation. Fifty milliliters was stained with DAPI, filtered onto 1.0-microm polycarbonate filters and viewed under epifluorescence microscopy using both blue and UV filters to determine heterotrophic and autotrophic flagellate abundances. The larger protozooplankton in each 1-l sample of glutaraldehyde-fixed water were concentrated by settling, stained with bromophenol blue and counted in Sedgewick-Rafter counting chambers under DIC microscopy at x320. Detailed analysis was undertaken under higher magnification using both DIC and phase microscopy. For phytoplankton analysis between 5 and 50 ml was subsampled. These phytoplankton were settled and enumerated using the inverted microscope method of Utermohl. Water was collected using either a Kemmerer bottle or a Van Dorn sampler lowered through a hole in the 4-m-thick ice with a Jiffy-drill in January 1992 and in January 1994 at a center station (depth 18.5 m). Samples were taken at 1-m intervals down to 12 m into the anoxic zone. In 1992, 250-ml subsamples were fixed in Lugol's iodine for the analysis of ciliates, and other aliquots fixed in glutaraldehyde for bacterial counts. In1994, 1.1-l samples were fixed in buffered glutaraldehyde for protozoological and bacterial analysis. These were kept refrigerated in the dark and flown to Australia or New Zealand for immediate analysis. Both fixatives are regarded as reliable for protistan analysis provided samples are analysed within a few months of collection. However, in our experience while Lugol's iodine is considered best for preserving abundance, it does cause more cell distortion of some species than glutaraldehyde, which gives better preservation of ciliates in freshwater samples. One liter of water from each depth was preserved in Lugol's iodine for phytoplankton counts. Temperature was measured with a (YSI) Yellow Springs Instruments 54 meter and probe, and conductivity with a Radiometer Conductivity meter. Ten milliliters of the buffered glutaraldehyde-fixed material was stained with DAPI, and filtered onto a 0.2-microm black polycarbonate filter, and bacterial numbers were counted under epifluorescence microscopy using UV excitation. Fifty milliliters was stained with DAPI, filtered onto 1.0-microm polycarbonate filters and viewed under epifluorescence microscopy using both blue and UV filters to determine heterotrophic and autotrophic flagellate abundances. The larger protozooplankton in each 1-l sample of glutaraldehyde-fixed water were concentrated by settling, stained with bromophenol blue and counted in Sedgewick-Rafter counting chambers under DIC microscopy at x320. Detailed analysis was undertaken under higher magnification using both DIC and phase microscopy. For phytoplankton analysis between 5 and 50 ml was subsampled. These phytoplankton were settled and enumerated using the inverted microscope method of Utermohl. unknown ciliates Location Name of lake where measurement was made The data provider Name of lake where measurement was made Date_TIME Date on which sample was gathered The data provider calendar date/time mm/dd/yyyy gregorian calendar Depth Depth at which sample was drawn from lake The data provider 1 12 meter 1 #Ciliates Number of ciliates counted/liter of lake water The data provider #/liter #Askenasia Number of Askenasia organisms counted/liter of lake water The data provider 0 #/liter 1 #S_Monodinium Number of S_Monodinium organisms counted/liter of lake water The data provider 0 #/liter 1 #L_Monodinium Number of L_Monodinium organisms counted/liter of lake water The data provider 0 #/liter 1 #Strombidium Number of Strombidium organisms counted/liter of lake water The data provider 0 #/liter 1 #Vorticella Number of Vorticella organisms counted/liter of lake water The data provider 0 #/liter 1 #Bursaria Number of Bursaria organisms counted/liter of lake water The data provider 0 #/liter 1 #Sphaerophyra Number of Sphaerophyra organisms counted/liter of lake water The data provider 0 #/liter 1 #Bacteria Number of bacteria x 10E8 counted/liter of lake water The data provider 0 #x108/liter 1 #HNAN Number of heterotrophic nanoflagellates x 10E5 counted/liter of lake water The data provider 0 #x105/liter 1 #PNAN Number of phototrophic nanoflagellates x 10E5 counted/liter of lake water The data provider 0 #x105/liter 1 McMurdo Dry Valleys LTER The data distributor shall not be liable for innacuracies in the content http 1 0 \n 1 column , https://mcm.lternet.edu/sites/default/files/ciliates.csv None 2014-11-04 2014-11-04 McMurdo Dry Valleys LTER http://mcmlter.org/ Biological Data Profile of the Content Standards for Digital Geospatial Metadata devised by the Federal Geographic Data Committee. Drupal Ecological information Management Systems, version D7, Biological Data Profile module