uid=MCM,o=EDI,dc=edirepository,dc=org all public read ciliates Johanna Laybourn-Parry

University Road, Clifton Bristol BS8 1SS Bristol - None - BS8 1S GB

jo.laybourn-parry@bristol.ac.uk McMurdo Dry Valleys LTER http://mcmlter.org/ Inigo San Gil

Department of Biology, MSC03 2020 University of New Mexico Albuquerque NM 87131 US

(505) 277-2625 (505) 277-2541 isangil@lternet.edu data manager 2014-11-10 English

In conjunction with the Long Term Ecological Research (LTER) project in the McMurdo Dry Valleys of Antarctica, lakes were monitored for microzooplankton by a team based out of the University of Nottingham (led by Johanna Laybourn-Parry). This dataset shows numbers of ciliates, bacteria, heterotrophic nanoflagellates, and phototrophic nanoflagellates found at various depths in Lake Fryxell.

bacteria bacterial abundance LTER Controlled Vocabulary Antarctica bacteria ciliate flagellate heterotrophic lake limnology microzooplankton nanoflagellate phototrophic Station Keywords population dynamics LTER Core Areas

This data package is released under the Creative Commons Attribution 4.0 International License (CC BY 4.0; http://creativecommons.org/licenses/by/4.0/), which allows consumers (hereinafter referred to as “Data Users”) to freely reuse, redistribute, transform, or build on this work (even commercially) so long as appropriate credit is provided. Accordingly, Data Users are required to properly cite this data package in any publications or in the metadata of any derived products that result from its use (in whole or in part). A recommended citation is provided on the summary metadata page associated with this data package in the McMurdo Dry Valleys LTER Data Catalog (https://mcmlter.org/data), and a generic citation may be found on the summary metadata page in the repository where this data package was obtained. When these data contribute significantly to the contents of a publication, Data Users must also acknowledge that data were provided by the NSF-supported McMurdo Dry Valleys Long Term Ecological Research program (OPP-2224760). This data package has been released in the spirit of open scientific collaboration. Hence, Data Users are strongly encouraged to consider consultation, collaboration, and/or co-authorship (as appropriate) with the data package creator(s). Data Users should be aware these data may be actively used by others for ongoing research; thus, coordination may be necessary to prevent duplicate publication. Data Users should also recognize that misinterpretation of data may occur if they are used outside the context of the original study. Hence, Data Users are urged to contact the data package creator(s) if they have any questions regarding methodology or results. While substantial efforts are made to ensure the accuracy of this data package (with all its components), complete accuracy cannot be guaranteed. Periodic updates to this data package may occur, and it is the responsibility of Data Users to check for new versions. This data package is made available “as is” and comes with no warranty of accuracy or fitness for use. The creator(s) of this data package and the repository where these data were obtained shall not be liable for any damages resulting from misinterpretation, use, or misuse of these data. Finally, as a professional courtesy, we kindly request Data Users notify the primary contact referenced in the metadata when these data are used in the production of any derivative work or publication. Notification should include an explanation of how the data were used, along with a digital copy of the derived product(s). Thank you.

https://mcm.lternet.edu/content/microzooplankton-lake-fryxell-ciliates-bacteria-nanoflagellates The Lake Fryxell basin is formed by a moraine depression in a wider portion of the Taylor Valley. It has a number of moraine islands and shallower areas, as well as several relatively well developed deltas. The lake is fed by at least 10 meltwater streams with a total drainage catchment of 230 km2. The lake is dammed to the southwest by the Canada Glacier and is topographically closed. It is perennially ice covered; during summer months, an ice-free moat generally forms around much of the lake margin. Lake levels have risen ~2 m between 1971 and 1996. There are no surface outflows; the only known water loss is through ice ablation (evaporation, sublimation and physical scouring). Valley: Taylor Distance to Sea : 9 Maximum Length (km): 5.8 Maximum Width (km): 2.1 Maximum Depth (m): 20 Surface Area (km^2): 7.08 Ice Thickness Average Surface (m): 3.3 - 4.5 Volume (m^3 * 10^6): 25.2 163.048782348633 163.259582519531 -77.597076416016 -77.622711181641 18 18 meter 1992-01-01 1994-01-31 Metadata completed in 2016. Data migrated to the Drupal Ecological Information Management System (DEIMS in 2014 (San Gil) Data for this file was submitted by Johanna Laybourn-Parry to the data manager at INSTAAR on October 21, 1998. Files were sent via e-mail as well as a hard copy. The original version of the file is stored on the Unix system in "/data1/data/lakes/plankton/laybourn-parry/Fryxell". Upon arrival at INSTAAR, the data manager reformatted the file to present it in a relational mode. This was done using Microsoft Access. It was then exported in comma delimited ascii and MS-DOS text format to present on the web. Links to these files are provided above. McMurdo Dry Valleys LTER http://mcmlter.org/ McMurdo Dry Valleys LTER http://mcmlter.org/ McMurdo Dry Valleys LTER

Water was collected using either a Kemmerer bottle or a Van Dorn sampler lowered through a hole in the 4-m-thick ice with a Jiffy-drill in January 1992 and in January 1994 at a center station (depth 18.5 m). Samples were taken at 1-m intervals down to 12 m into the anoxic zone. In 1992, 250-ml subsamples were fixed in Lugol's iodine for the analysis of ciliates, and other aliquots fixed in glutaraldehyde for bacterial counts. In1994, 1.1-l samples were fixed in buffered glutaraldehyde for protozoological and bacterial analysis. These were kept refrigerated in the dark and flown to Australia or New Zealand for immediate analysis. Both fixatives are regarded as reliable for protistan analysis provided samples are analysed within a few months of collection. However, in our experience while Lugol's iodine is considered best for preserving abundance, it does cause more cell distortion of some species than glutaraldehyde, which gives better preservation of ciliates in freshwater samples. One liter of water from each depth was preserved in Lugol's iodine for phytoplankton counts. Temperature was measured with a (YSI) Yellow Springs Instruments 54 meter and probe, and conductivity with a Radiometer Conductivity meter. Ten milliliters of the buffered glutaraldehyde-fixed material was stained with DAPI, and filtered onto a 0.2-microm black polycarbonate filter, and bacterial numbers were counted under epifluorescence microscopy using UV excitation. Fifty milliliters was stained with DAPI, filtered onto 1.0-microm polycarbonate filters and viewed under epifluorescence microscopy using both blue and UV filters to determine heterotrophic and autotrophic flagellate abundances. The larger protozooplankton in each 1-l sample of glutaraldehyde-fixed water were concentrated by settling, stained with bromophenol blue and counted in Sedgewick-Rafter counting chambers under DIC microscopy at x320. Detailed analysis was undertaken under higher magnification using both DIC and phase microscopy. For phytoplankton analysis between 5 and 50 ml was subsampled. These phytoplankton were settled and enumerated using the inverted microscope method of Utermohl.

ciliates ciliates.csv 1055 1 0 \n column , " https://mcm.lternet.edu/sites/default/files/ciliates.csv Location Name Location Name of lake where measurement was made string Name of lake where measurement was made Date_TIME Date_TIME Date on which sample was gathered date mm/dd/yyyy Depth Depth Depth at which sample was drawn from lake meter 1 real 1 12 #Ciliates #Ciliates Number of ciliates counted/liter of lake water #/liter real Required entry None given #Askenasia #Askenasia Number of Askenasia organisms counted/liter of lake water #/liter 1 real 0 #S_Monodinium #S_Monodinium Number of S_Monodinium organisms counted/liter of lake water #/liter 1 real 0 Required entry None given #L_Monodinium #L_Monodinium Number of L_Monodinium organisms counted/liter of lake water #/liter 1 real 0 Required entry None given #Strombidium #Strombidium Number of Strombidium organisms counted/liter of lake water #/liter 1 real 0 Required entry None given #Vorticella #Vorticella Number of Vorticella organisms counted/liter of lake water #/liter 1 real 0 Required entry None given #Bursaria #Bursaria Number of Bursaria organisms counted/liter of lake water #/liter 1 real 0 Required entry None given #Sphaerophyra #Sphaerophyra Number of Sphaerophyra organisms counted/liter of lake water #/liter 1 real 0 Required entry None given #Bacteria #Bacteria Number of bacteria x 10E8 counted/liter of lake water #x108/liter 1 real 0 Required entry None given #HNAN #HNAN Number of heterotrophic nanoflagellates x 10E5 counted/liter of lake water #x105/liter 1 real 0 Required entry None given #PNAN #PNAN Number of phototrophic nanoflagellates x 10E5 counted/liter of lake water #x105/liter 1 real 0 Required entry None given