dpah94 English utf8 dataset McMurdo Dry Valleys LTER http://mcmlter.org/ 2014-11-11 ISO 19115-2 Geographic Information - North American Profile Metadata - Data with Biological Extensions ISO 19115-2:2009(E) Soil Depth Effects on Anhydrobiosis of Nematodes 2014-11-11 publication Diana Wall Colorado State University http://www.colostate.edu Johnson Hall 107 Fort Collins CO 80523 US Diana.Wall@colostate.edu http://wp.natsci.colostate.edu/walllab/ pointOfContact Ross Virginia Dartmouth College http://dartmouth.edu/ (603) 646-0192 Hinman Box 6182 Hanover NH 03755 US ross.a.virginia@dartmouth.edu http://sites.dartmouth.edu/ravirginia/ pointOfContact documentDigital Investigation of the effect of soil depth on soil biota and properties was part of the McMurdo Dry Valleys Long Term Ecological Research (LTER) project. The proportion of soil nematodes found in an anhydrobiotic survival state was monitored at various soil depths in Taylor Valley to help accomplish this. Samples were taken on 21-Nov-1994 and 26-Dec-1994. Name: Denise Steigerwald Role: data manager Name: Inigo San Gil Role: data manager completed McMurdo Dry Valleys LTER http://mcmlter.org/ unknown This file was created by Mark St. John on 14-Oct-1998, using raw data from the Excel workbooks '9411dpah.raw', and '9412dpah.raw'. The file format was suggested by the LTER data manager, to conform with the relational database structure. On 19 Oct 1998, these files were submitted to Denise Steigerwald, the MCM LTER data manager, located at INSTAAR, University of Colorado. Upon arrival at INSTAAR, the data manager combined the 2 data files, removed columns for latitude and longitude, and updated the location names to match those provided in the "soil measurement locations" file (from which latitude and longitude can be found). In addition, a column for depth range was added in order to make it possible to compare soil moisture, chlorophyll-a, and soil nematode densities found at a given depth, but stored in different data files (dpca94, dpah94, dpso9394, and dpwo9394 files). The resulting file was reformatted to present in ascii, comma delimited text and MS-DOS text (table layout) on the MCM LTER web site. Both of these files are linked to this web page above. population dynamics theme LTER Core Areas English Long Term Manipulation Experiment at South Side of Lake Hoare LTM 162.882125854492 162.882125854492 -77.632965087891 -77.632965087891 ground condition 1994-11-21 1994-12-26 https://mcm.lternet.edu/sites/default/files/dpah94.csv dpah94 eng; US McMurdo Dry Valleys LTER dpah94 Record Delimiter : \n Number of Header Lines : 1 Number of Footer Lines : 0 Orientation : Column Quote Character : "Field Delimiter : , false LOCATION Name of area where measurement was made DATE_TIME Date on which sample was gathered Date Time Format: mm/dd/yyyy Depth range (cm) Minimum and maximum soil depth from which sample was drawn 0 - 2.5 Depth ranges from 0 to 2.5cm 2.5 - 5 Depth ranges from 2.5 to 5cm 5 - 7.5 Depth ranges from 5 to 7.5cm 7.5 - 10 Depth ranges from 7.5 to 10cm 10 - 15 Depth ranges from 10 to 15cm 15 - 20 Depth ranges from 15 to 20cm 20 - 25 Depth ranges from 20 to 25cm SAMPLE # Sample ID % COILED NEMATODES Percentage of the nematodes found in a coiled state FILE NAME Name of file in which data was stored DBF https://mcm.lternet.edu/sites/default/files/dpah94.csv dataset   Soil samples were taken for organism enumeration and moisture content analysis as follows: Sampling bags were prepared with one sterile 'Whirlpak' bag and clean plastic scoop per sample.  Centers of 8 polygons were found and labelled "A" to "H".  Samples were taken from within a 10 cm diameter circular area of each plot.  The location of the sampling was recorded each year so that areas were not re-sampled.  A hole approximately 20 cm deep was dug at the center of each polygon.  Samples were taken at depths 0-2.5 cm, 2.5-5 cm, 5-10 cm and 10-20 cm by digging in from the sides.  Very large  rocks (>20 mm diameter) were excluded from the sample.  The soil was shoveled into the 'Whirlpak' bag until three quarters full (about 1.5 kg soil).  The soil was mixed well in the bag, then the bag was closed tightly, expelling as much air as possible.  The soil samples were stored in a cooler for transportation.  On return to the laboratory (within 8 hours of sampling), the soils were stored at -8 degrees C until further processing.   In the laboratory, soil samples were handled in a laminar flow hood to prevent contamination.  The Whirlpak bags of soil were mixed thoroughly prior to opening.  The remaining soil in the plastic beaker was weighed.  100g of soil (less rocks) were measured into a beaker and brought up to 250 mL with a 1.25M sucrose solution.  Mixture was star stirred using a spatula for 30 seconds.  Immediately the liquid was poured into screens-a stack of 40 mesh on top of a 400 mesh, prewetted with 1.25M sucrose solution.  The screens were rinsed gently with 1.25M sucrose solution (from a wash bottle) through the top of the stack, keeping the screens at an angle as the solution filtered through.  The solution was kept on ice at all times.  The top screen was removed, and the lower screen rinsed top down, never directly on top of the soil, but at the top of the screen and from behind.  The sucrose solution was allowed to cascade down and carry the particles into the bottom wedge of the angled screen.  The side of the screen was tapped gently to filter all the solution through.  The suspension was rinsed from the front and the back, keeping the screen at an angle and not allowing the solution to overflow the edge of the screen.  The bottom screen was then rinsed into a 150 mL beaker with 1.25M sucrose, from the front, using a funnel.  The sugar and sediment were then slowly pipetted, using an automatic pipetter, from the beaker into a centrifuge tube containing 10 mL of 2M sugar on ice, at an angle to retain the boundary between sugar layers.  The beaker was rinsed with 1.25 sucrose and the dregs were pipetted as well.  As many centrifuge tubes as was necessary per sample were used. Each tube was evened off with 1.25M sucrose and centifuged for 5 minutes at 1744 RPM. The supernatant was then poured through a prewetted (with 1.25M sucrose solution) 500 mesh screen, stopping before the sediment came out and rinsed well with ice cold 1.25M sucrose and backwashed into a centrifuge tube.  Samples were refrigerated at 5 degrees C until counted.     Samples were washed with 1.25M sucrose into a counting dish and examined under a microscope at x10 or x20 magnification.  Nematodes were identified as coiled or uncoiled.  Total numbers in each sample were recorded on data sheets.  Data were entered into Excel files, printed, and checked for errors.   Metadata Access Constraints: none Metadata Use Constraints: none annually McMurdo Dry Valleys LTER http://mcmlter.org/ pointOfContact