Soil Properties Effects on Soil Biota

spwo Soil Properties Effects on Soil Biota Diana Wall

Johnson Hall 107 Fort Collins CO 80523 US

Diana.Wall@colostate.edu http://wp.natsci.colostate.edu/walllab/ https://orcid.org/0000-0002-9466-5235 Ross Virginia

Hinman Box 6182 Hanover NH 03755 US

(603) 646-0192 ross.a.virginia@dartmouth.edu http://sites.dartmouth.edu/ravirginia/ https://orcid.org/0000-0002-0890-0981 McMurdo Dry Valleys LTER http://mcmlter.org/ 2014-11-12 English

Investigation of the effect of short-term variation in soil moisture and soil temperature on nematode anhydrobiosis as part of the McMurdo Dry Valleys Long Term Ecological Research (LTER) project. The percent of anhydrobiotic (coiled) nematodes with relation to soil moisture, temperature, and salinity was determined. The study began in the austral summer of 1996/1997.

biomass nematodes rotifers soil soil disturbance LTER Controlled Vocabulary Antarctica biomass biotic effects Eudorylaimus LTER nematodes Plectus rotifers Scottnema soil soil biota Station Keywords population dynamics LTER Core Areas

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https://mcm.lternet.edu/content/soil-properties-effects-soil-biota This is the plot for the Biotic Effects Experiment at Lake Hoare 162.876007080078 162.876007080078 -77.635002136230 -77.635002136230 Biotic Effects Experiment Fryxell Plot 163.248001098633 163.248001098633 -77.608001708984 -77.608001708984 Moss Sample 28 @ Canada Glacier ASPA. 163.024368286133 163.024368286133 -77.612335205078 -77.612335205078 1997-01-01 Dataset updated in 2016 - San Gil. This file was created by Pilar Tillberg on 10 May 2001, using raw data from the Excel workbook '9701.raw'. The file format was suggested by the LTER data manager, to conform with the relational database structure. [PT 10 May 2001]. McMurdo Dry Valleys LTER http://mcmlter.org/ McMurdo Dry Valleys LTER http://mcmlter.org/ McMurdo Dry Valleys LTER

Soil samples were collected from three sites: 30 samples from the south side of Lake Hoare sampled in a 6m2 area; 30 samples from the south side of Lake Fryxell sampled in a 6m2 area; and 27 samples in the Site of Special Scientific Interest #12 collected along three 100-m transects extending away from glacier meltwater. Soil samples were taken for organism enumeration and moisture content analysis as follows: Sampling bags were prepared with one sterile 'Whirlpak' bag and clean plastic scoop per sample. Samples were taken from within the 10 cm diameter circular area of each plot. The location of the sampling was recorded each year so that areas were not re-sampled. Using the plastic scoop, soil was collected to 10 cm depth. Very large rocks ( greater than 20 mm diameter) were excluded from the sample. The soil was shoveled into the 'Whirlpak' bag until three quarters full (about 1.5 kg soil). The soil was mixed well in the bag, then the bag was closed tightly, expelling as much air as possible. The soil samples were stored in a cooler for transportation. On return to the laboratory (within 8 hours of sampling), the soils were stored at 4C until further processing. In the laboratory, soil samples were handled in a laminar flow hood to prevent contamination. The Whirlpak bags of soil were mixed thoroughly prior to opening. Approximately 200cm3 of soil was placed in a pre-weighed 800mL plastic beaker. Rocks greater than 3-4mm in diameter were removed from the sample. A sub-sample of approximately 50g was removed and placed in a pre-weighed aluminum dish, and weighed on a balance accurate to 0.01g. This sample was dried at 105C for 24 hours. The sample was removed, placed in desiccator to cool down, and re-weighed. These data were used to calculate water content of the soil and to express data as numbers of soil organisms per unit dry weight of soil. The remaining soil in the plastic beaker was weighed. Cold tap water was added up to 650 mL. The soil suspension was stirred carefully (star stir or figure of 8) for 30 seconds, using a spatula. Immediately the liquid was poured into wet screens - a stack of 40 mesh on top of a 400 mesh. The screens were rinsed gently with ice cold tap water (from a wash bottle) through the top of the stack, keeping the screens at an angle as the water filtered through. The water was kept on ice at all times. The top screen was removed, and the lower screen rinsed top down, never directly on top of the soil, but at the top of the screen and from behind. The water was allowed to cascade down and carry the particles into the bottom wedge of the angled screen. The side of the screen was tapped gently to filter all the water through. The suspension was rinsed from the front and the back, keeping the screen at an angle and not allowing the water to overflow the edge of the screen. The soil particles were backwashed into a 50mL plastic centrifuge tube, tipping the screen into the funnel above the tube and rinsing the funnel gently. The suspension was centrifuged for five minutes at 1744 RPM. The liquid was decanted, leaving a few mL on top of the soil particles. The tube was filled with sucrose solution (454g sucrose per liter of tap water, kept refrigerated) up to 45mL. This was stirred gently with a spatula until the pellet was broken up and suspended. The suspension was centrifuged for one minute at 1744 RPM, decanted into a wet 500 mesh screen, rinsed well with ice cold tap water and backwashed into a centrifuge tube. Samples were refrigerated at 5C until counted. Samples were washed in to a counting dish and examined under a microscope at x10 or x20 magnification. Rotifers and tardigrades were identified and counted. Nematodes were identified to species and sex, and counted. Total numbers in each sample were recorded on data sheets. All species of nematode, and all rotifers and tardigrades found in the sample were recorded. Data were entered in to Excel files, printed, and checked for errors.

spwo Soil properties effects on soil biota data units and spreadsheet column descriptions spwo.csv 332636 1 0 \r\n column , " https://mcm.lternet.edu/sites/default/files/spwo.csv LOCATION LOCATION Name of area where measurement was made string Name of area where measurement was made DATE_TIME DATE_TIME Date on which sample was gathered date mm/dd/YYYY SAMPLE # SAMPLE # ID associated with transect, sampling location string ID associated with transect, sampling location TYPE OF ORGANISM TYPE OF ORGANISM Family associated with organism string Family associated with organism SPECIES SPECIES Species found in soil string Species found in soil SEX SEX Gender of organism (male vs. female) string Gender of organism (male vs. female) LIVE/DEAD/COMBINED LIVE/DEAD/COMBINED Survival Status (living, dead, or both) string Survival Status (living, dead, or both) ADULT/JUVENILE/COMBINED ADULT/JUVENILE/COMBINED Maturity (adult, juvenile, or both) string Maturity (adult, juvenile, or both) TOTAL (#/KG DRY SOIL) TOTAL (#/KG DRY SOIL) Number of organisms found in that category #/KG DRY SOIL real Comments Comments Comments string Comments FILE NAME FILE NAME Name of file in which data was stored string Name of file in which data was stored