uid=MCM,o=EDI,dc=edirepository,dc=org all public read rsca Diana Wall

Johnson Hall 107 Fort Collins CO 80523 US

Diana.Wall@colostate.edu http://wp.natsci.colostate.edu/walllab/ https://orcid.org/0000-0002-9466-5235 McMurdo Dry Valleys LTER http://mcmlter.org/ Ross Virginia

Hinman Box 6182 Hanover NH 03755 US

(603) 646-0192 ross.a.virginia@dartmouth.edu http://sites.dartmouth.edu/ravirginia/ https://orcid.org/0000-0002-0890-0981 associated researcher Inigo San Gil

Department of Biology, MSC03 2020 University of New Mexico Albuquerque NM 87131 US

(505) 277-2625 (505) 277-2541 isangil@lternet.edu data manager 2014-01-01 English

Investigation of the effect of hydrological and biogeochemical linkages in the transition zone between streams and surrounding soils on invertebrate community structure as part of the McMurdo Dry Valleys Long Term Ecological Research (LTER) project. The invertebrate community structure with relation to soil moisture, salinity, and chlorophyll-a was determined. The study took place on 28 November 1997 and 31 December 1997.

chlorophyll a soil soil disturbance soil moisture LTER Controlled Vocabulary Antarctica chlopophyl-A chlorophyll LTER soil soil biota Station Keywords population dynamics LTER Core Areas

This data package is released under the Creative Commons Attribution 4.0 International License (CC BY 4.0; http://creativecommons.org/licenses/by/4.0/), which allows consumers (hereinafter referred to as “Data Users”) to freely reuse, redistribute, transform, or build on this work (even commercially) so long as appropriate credit is provided. Accordingly, Data Users are required to properly cite this data package in any publications or in the metadata of any derived products that result from its use (in whole or in part). A recommended citation is provided on the summary metadata page associated with this data package in the McMurdo Dry Valleys LTER Data Catalog (https://mcmlter.org/data), and a generic citation may be found on the summary metadata page in the repository where this data package was obtained. When these data contribute significantly to the contents of a publication, Data Users must also acknowledge that data were provided by the NSF-supported McMurdo Dry Valleys Long Term Ecological Research program (OPP-2224760). This data package has been released in the spirit of open scientific collaboration. Hence, Data Users are strongly encouraged to consider consultation, collaboration, and/or co-authorship (as appropriate) with the data package creator(s). Data Users should be aware these data may be actively used by others for ongoing research; thus, coordination may be necessary to prevent duplicate publication. Data Users should also recognize that misinterpretation of data may occur if they are used outside the context of the original study. Hence, Data Users are urged to contact the data package creator(s) if they have any questions regarding methodology or results. While substantial efforts are made to ensure the accuracy of this data package (with all its components), complete accuracy cannot be guaranteed. Periodic updates to this data package may occur, and it is the responsibility of Data Users to check for new versions. This data package is made available “as is” and comes with no warranty of accuracy or fitness for use. The creator(s) of this data package and the repository where these data were obtained shall not be liable for any damages resulting from misinterpretation, use, or misuse of these data. Finally, as a professional courtesy, we kindly request Data Users notify the primary contact referenced in the metadata when these data are used in the production of any derivative work or publication. Notification should include an explanation of how the data were used, along with a digital copy of the derived product(s). Thank you.

https://mcm.lternet.edu/content/stream-transects-chlorophyll-concentration Parent Stream: Harnish Creek Tributary, also known as Relict Channel 163.294601440430 163.294601440430 -77.631713867188 -77.631713867188 On the Stream team data page 163.302963256836 163.302963256836 -77.632797241211 -77.632797241211 "Coordinates are same as those for RC1H, but samples taken from stream rather than hyporheic zone." Parent Stream: Harnish Creek Tributary, also known as Relict Channel 163.285720825195 163.285720825195 -77.631942749023 -77.631942749023 110 110 meter Transect by Von Guerard Stream at Lower Site Moss Site M-06 Transect 6 Points T061 and T062 mark the start and end of the transect. Start : IDs: 6VGLOWERT061 Ellipsoid: 33.99 ORTHOMETRIC HT (m) 89.19 End: ID: 6VGLOWERT062 Ellipsoid: 33.74 ORTHOMETRIC HT (m) 88.92 File provenance: strm-clg.txt 163.287460327148 163.288482666016 -77.619300842285 -77.619659423828 89 89 meter Von Guerard Stream at F21. Id: uvg_f21.This was an ill-fated upstream gage on Von Guerard, 2.5 kilometers upstream from F6.  It was installed during the lowest season of record (00-01) and then promptly destroyed during the 01-02 season.  It's parts were stripped and it never generated a hydrology database, but was certainly sampled. 163.301254272461 163.301254272461 -77.626380920410 -77.626380920410 0 0 meter 1997-11-28 1997-12-31 McMurdo Dry Valleys LTER http://mcmlter.org/ McMurdo Dry Valleys LTER http://mcmlter.org/ McMurdo Dry Valleys LTER

 Approximately 1500g of soil or sediment were collected into Whirl-Pak(tm) bags with clean plastic scoops from 5 sections (0, 8, 16, 24, and 32 m away from the stream) along each transect. Five grams of sediment/soil were also collected from the top 1 cm for measurement of chlorophyll-a concentrations. Samples were mixed within the bags, placed into insulated coolers, and transported for analysis to the McMurdo laboratories. Until processing, the soils were stored in a 4_C refrigerator. Samples were analyzed by ASA Analytical Services on January 6th 1998.   Extraction of chlorophyll from the soil. All procedures were carried out in the dark or very low irradiance to avoid degradation of the chlorophyll. The soil samples were mixed thoroughly in the vials, and a sample of approximately 5 g was weighed out in to a 50 mL plastic centrifuge tube with a screw-top cap. 10 mL of a 50:50 DMSO/90% acetone solution was added to each sample and they were mixed thoroughly on a bench-top Vortex mixer for about 5 seconds. The vials were placed in a -4C constant temperature room, in the dark, and left for 12-18 hours.   Determination of chlorophyll a concentration. This was determined fluorometrically using a Turner model 111 fluorometer. A calibration using a known concentration of chlorophyll was carried out prior to sample analysis. The machine was blanked using a 50:50 DMSO/90% acetone solution. Each vial was mixed thoroughly, then centrifuged for 5 minutes at about 1800 RPM. A sample of approximately 4mL of the DMSO/acetone solution was taken from the top of the sample with a pipette, being careful not to get any soil particles in the solution. The sample was placed in a cuvette, in to the fluorometer and the fluorescence was recorded . This was done fairly quickly in order to prevent light from breaking down the chlorophyll. This measurement is called Fo, the initial fluorescence. After taking this reading, 0.1 mL of 1N HCl was added directly to the cuvette and the cuvette was gently agitated. After 20 seconds, the fluorescence was re-measured.   (During this step, the acid converts the chlorophyll to phaeophytin by releasing a magnesium ion in an acidic environment). This measurement is called Fa, the fluorescence after acidification. The solution was discarded in to a waste container, and the cuvette rinsed 3 times with DMSO/90% acetone solution before proceeding with the next sample.  Data were received at NREL on disk and hard copy in the spring of 1998.  

rsca Comma delimited spreadsheet containing the rsca data described here rsca.csv 3394 1 0 \n column , " https://mcm.lternet.edu/sites/default/files/rsca.csv LOCATION LOCATION Name of area where measurement was made string Name of area where measurement was made DATE_TIME Date Date on which sample was gathered date mm/dd/yyyy SAMPLE # SAMPLE # Sample ID string Sample ID CHL-A (uG/G SOIL) CHLOROPHYLL A (MICROG/G SOIL) Chlorophyll a concentration found in soil microgramsPerGram real COMMENTS COMMENTS Helpful hints about the sample string Helpful hints about the sample FILE NAME FILE NAME Name of file in which data was stored string Name of file in which data was stored