rsca English utf8 dataset McMurdo Dry Valleys LTER http://mcmlter.org/ 2014-11-12 ISO 19115-2 Geographic Information - North American Profile Metadata - Data with Biological Extensions ISO 19115-2:2009(E) Stream Transects - Chlorophyll-A Concentration 2014-11-12 publication Diana Wall Colorado State University http://www.colostate.edu Johnson Hall 107 Fort Collins CO 80523 US Diana.Wall@colostate.edu http://wp.natsci.colostate.edu/walllab/ pointOfContact documentDigital Investigation of the effect of hydrological and biogeochemical linkages in the transition zone between streams and surrounding soils on invertebrate community structure as part of the McMurdo Dry Valleys Long Term Ecological Research (LTER) project. The invertebrate community structure with relation to soil moisture, salinity, and chlorophyll-a was determined. The study took place on 28 November 1997 and 31 December 1997. Name: Ross A. Virginia Role: associated researcher Name: Inigo San Gil Role: data manager completed McMurdo Dry Valleys LTER http://mcmlter.org/ population dynamics theme LTER Core Areas English Parent Stream: Harnish Creek Tributary, also known as Relict Channel 163.294601440430 163.294601440430 -77.631713867188 -77.631713867188 On the Stream team data page 163.302963256836 163.302963256836 -77.632797241211 -77.632797241211 "Coordinates are same as those for RC1H, but samples taken from stream rather than hyporheic zone." Parent Stream: Harnish Creek Tributary, also known as Relict Channel 163.285720825195 163.285720825195 -77.631942749023 -77.631942749023 Transect by Von Guerard Stream at Lower Site Moss Site M-06 Transect 6 Points T061 and T062 mark the start and end of the transect. Start : IDs: 6VGLOWERT061 Ellipsoid: 33.99 ORTHOMETRIC HT (m) 89.19 End: ID: 6VGLOWERT062 Ellipsoid: 33.74 ORTHOMETRIC HT (m) 88.92 File provenance: strm-clg.txt 163.288482666016 163.287460327148 -77.619659423828 -77.619300842285 Von Guerard Stream at F21. Id: uvg_f21.This was an ill-fated upstream gage on Von Guerard, 2.5 kilometers upstream from F6.  It was installed during the lowest season of record (00-01) and then promptly destroyed during the 01-02 season.  It's parts were stripped and it never generated a hydrology database, but was certainly sampled. 163.301254272461 163.301254272461 -77.626380920410 -77.626380920410 ground condition 1997-11-28 1997-12-31 https://mcm.lternet.edu/sites/default/files/rsca.csv rsca eng; US McMurdo Dry Valleys LTER rsca Data Source Definition : Comma delimited spreadsheet containing the rsca data described here Record Delimiter : \n Number of Header Lines : 1 Number of Footer Lines : 0 Orientation : Column Quote Character : "Field Delimiter : , false LOCATION Name of area where measurement was made DATE_TIME Date on which sample was gathered Date Time Format: mm/dd/yyyy SAMPLE # Sample ID CHL-A (uG/G SOIL) Chlorophyll a concentration found in soil COMMENTS Helpful hints about the sample FILE NAME Name of file in which data was stored DBF https://mcm.lternet.edu/sites/default/files/rsca.csv dataset  Approximately 1500g of soil or sediment were collected into Whirl-Pak(tm) bags with clean plastic scoops from 5 sections (0, 8, 16, 24, and 32 m away from the stream) along each transect. Five grams of sediment/soil were also collected from the top 1 cm for measurement of chlorophyll-a concentrations. Samples were mixed within the bags, placed into insulated coolers, and transported for analysis to the McMurdo laboratories. Until processing, the soils were stored in a 4_C refrigerator. Samples were analyzed by ASA Analytical Services on January 6th 1998.   Extraction of chlorophyll from the soil. All procedures were carried out in the dark or very low irradiance to avoid degradation of the chlorophyll. The soil samples were mixed thoroughly in the vials, and a sample of approximately 5 g was weighed out in to a 50 mL plastic centrifuge tube with a screw-top cap. 10 mL of a 50:50 DMSO/90% acetone solution was added to each sample and they were mixed thoroughly on a bench-top Vortex mixer for about 5 seconds. The vials were placed in a -4C constant temperature room, in the dark, and left for 12-18 hours.   Determination of chlorophyll a concentration. This was determined fluorometrically using a Turner model 111 fluorometer. A calibration using a known concentration of chlorophyll was carried out prior to sample analysis. The machine was blanked using a 50:50 DMSO/90% acetone solution. Each vial was mixed thoroughly, then centrifuged for 5 minutes at about 1800 RPM. A sample of approximately 4mL of the DMSO/acetone solution was taken from the top of the sample with a pipette, being careful not to get any soil particles in the solution. The sample was placed in a cuvette, in to the fluorometer and the fluorescence was recorded . This was done fairly quickly in order to prevent light from breaking down the chlorophyll. This measurement is called Fo, the initial fluorescence. After taking this reading, 0.1 mL of 1N HCl was added directly to the cuvette and the cuvette was gently agitated. After 20 seconds, the fluorescence was re-measured.   (During this step, the acid converts the chlorophyll to phaeophytin by releasing a magnesium ion in an acidic environment). This measurement is called Fa, the fluorescence after acidification. The solution was discarded in to a waste container, and the cuvette rinsed 3 times with DMSO/90% acetone solution before proceeding with the next sample.  Data were received at NREL on disk and hard copy in the spring of 1998.   Metadata Access Constraints: none Metadata Use Constraints: none annually McMurdo Dry Valleys LTER http://mcmlter.org/ pointOfContact