uid=MCM,o=EDI,dc=edirepository,dc=org all public read rswo Diana Wall

Johnson Hall 107 Fort Collins CO 80523 US

Diana.Wall@colostate.edu http://wp.natsci.colostate.edu/walllab/ https://orcid.org/0000-0002-9466-5235 McMurdo Dry Valleys LTER http://mcmlter.org/ Ross Virginia

Hinman Box 6182 Hanover NH 03755 US

(603) 646-0192 ross.a.virginia@dartmouth.edu http://sites.dartmouth.edu/ravirginia/ https://orcid.org/0000-0002-0890-0981 associated researcher Denise Steigerwald data manager Inigo San Gil

Department of Biology, MSC03 2020 University of New Mexico Albuquerque NM 87131 US

(505) 277-2625 (505) 277-2541 isangil@lternet.edu data manager 2014-01-01 English

Investigation of the effect of hydrological and biogeochemical linkages in the transition zone between streams and surrounding soils on invertebrate community structure as part of the McMurdo Dry Valleys Long Term Ecological Research (LTER) project. The invertebrate community structure with relation to soil moisture, salinity, and chlorophyll-a was determined. The study took place on 28 November 1997 and 31 December 1997.

biomass chlorophyll a nematodes rotifers salinity soil moisture LTER Controlled Vocabulary population dynamics LTER Core Areas

This data package is released under the Creative Commons Attribution 4.0 International License (CC BY 4.0; http://creativecommons.org/licenses/by/4.0/), which allows consumers (hereinafter referred to as “Data Users”) to freely reuse, redistribute, transform, or build on this work (even commercially) so long as appropriate credit is provided. Accordingly, Data Users are required to properly cite this data package in any publications or in the metadata of any derived products that result from its use (in whole or in part). A recommended citation is provided on the summary metadata page associated with this data package in the McMurdo Dry Valleys LTER Data Catalog (https://mcmlter.org/data), and a generic citation may be found on the summary metadata page in the repository where this data package was obtained. When these data contribute significantly to the contents of a publication, Data Users must also acknowledge that data were provided by the NSF-supported McMurdo Dry Valleys Long Term Ecological Research program (OPP-2224760). This data package has been released in the spirit of open scientific collaboration. Hence, Data Users are strongly encouraged to consider consultation, collaboration, and/or co-authorship (as appropriate) with the data package creator(s). Data Users should be aware these data may be actively used by others for ongoing research; thus, coordination may be necessary to prevent duplicate publication. Data Users should also recognize that misinterpretation of data may occur if they are used outside the context of the original study. Hence, Data Users are urged to contact the data package creator(s) if they have any questions regarding methodology or results. While substantial efforts are made to ensure the accuracy of this data package (with all its components), complete accuracy cannot be guaranteed. Periodic updates to this data package may occur, and it is the responsibility of Data Users to check for new versions. This data package is made available “as is” and comes with no warranty of accuracy or fitness for use. The creator(s) of this data package and the repository where these data were obtained shall not be liable for any damages resulting from misinterpretation, use, or misuse of these data. Finally, as a professional courtesy, we kindly request Data Users notify the primary contact referenced in the metadata when these data are used in the production of any derivative work or publication. Notification should include an explanation of how the data were used, along with a digital copy of the derived product(s). Thank you.

https://mcm.lternet.edu/content/stream-transects-soil-biota Also referred to as 'RC Dev' Parent Stream: Harnish Creek Tributary, also known as Relict Channel 163.283615112305 163.283615112305 -77.631050109863 -77.631050109863 100 100 meter Von Guerard Stream at F21. Id: uvg_f21.This was an ill-fated upstream gage on Von Guerard, 2.5 kilometers upstream from F6.  It was installed during the lowest season of record (00-01) and then promptly destroyed during the 01-02 season.  It's parts were stripped and it never generated a hydrology database, but was certainly sampled. 163.301254272461 163.301254272461 -77.626380920410 -77.626380920410 0 0 meter USGS site 5; coordinates taken from 1996-97 GPS measurements at center of weir Parent Stream: Von Guerard Stream Provenance : GPS96-97.DOC ID: vguerard_f6 163.253997802734 163.253997802734 -77.608200073242 -77.608200073242 19 19 meter Parent Stream: Harnish Creek Tributary, also known as Relict Channel 163.294601440430 163.294601440430 -77.631713867188 -77.631713867188 Parent Stream: Harnish Creek Tributary, also known as Relict Channel 163.294113159180 163.294113159180 -77.633735656738 -77.633735656738 130 130 meter Relict Channel at Site_3 163.249588012695 163.249588012695 -77.615768432617 -77.615768432617 1997-11-28 1997-12-31 Readied for distribution and assigned DOI in 2016, San Gil.   This file was created by Pilar Tillberg on 11 May 2001, using raw data from the Excel workbook '9711rswo.raw'. The file format was suggested by the LTER data manager, to conform with the relational database structure. [PT 11 May 2001]. McMurdo Dry Valleys LTER http://mcmlter.org/ McMurdo Dry Valleys LTER http://mcmlter.org/ McMurdo Dry Valleys LTER

Approximately 1500g of soil or sediment were collected into Whirl-Pak(tm) bags with clean plastic scoops from 5 sections (0, 8, 16, 24, and 32 m away from the stream) along each transect. Five grams of sediment/soil were also collected from the top 1 cm for measurement of chlorophyll-a concentrations. Samples were mixed within the bags, placed into insulated coolers, and transported for analysis to the McMurdo laboratories. The soils were processed within 24 days of collection from the field. Until then, the soils were stored in a 4_C refrigerator. In the laboratory, soil samples were handled in a laminar flow hood to prevent contamination. The Whirlpak bags of soil were mixed thoroughly prior to opening. Approximately 200cm3 of soil was placed in a pre-weighed 800mL plastic beaker. Rocks greater than 3-4mm in diameter were removed from the sample. A sub-sample of approximately 50g was removed and placed in a pre-weighed aluminum dish, and weighed on a balance accurate to 0.01g. This sample was dried at 105C for 24 hours. The sample was removed, placed in desiccator to cool down, and re-weighed. These data were used to calculate water content of the soil and to express data as numbers of soil organisms per unit dry weight of soil. The remaining soil in the plastic beaker was weighed. Cold tap water was added up to 650 mL. The soil suspension was stirred carefully (star stir or figure of 8) for 30 seconds, using a spatula. Immediately the liquid was poured into wet screens - a stack of 40 mesh on top of a 400 mesh. The screens were rinsed gently with ice cold tap water (from a wash bottle) through the top of the stack, keeping the screens at an angle as the water filtered through. The water was kept on ice at all times. The top screen was removed, and the lower screen rinsed top down, never directly on top of the soil, but at the top of the screen and from behind. The water was allowed to cascade down and carry the particles into the bottom wedge of the angled screen. The side of the screen was tapped gently to filter all the water through. The suspension was rinsed from the front and the back, keeping the screen at an angle and not allowing the water to overflow the edge of the screen. The soil particles were backwashed into a 50mL plastic centrifuge tube, tipping the screen into the funnel above the tube and rinsing the funnel gently. The suspension was centrifuged for five minutes at 1744 RPM. The liquid was decanted, leaving a few mL on top of the soil particles. The tube was filled with sucrose solution (454g sucrose per liter of tap water, kept refrigerated) up to 45mL. This was stirred gently with a spatula until the pellet was broken up and suspended. The suspension was centrifuged for one minute at 1744 RPM, decanted into a wet 500 mesh screen, rinsed well with ice cold tap water and backwashed into a centrifuge tube. Samples were refrigerated at 5C until counted. Samples were washed in to a counting dish and examined under a microscope at x10 or x20 magnification. Rotifers and tardigrades were identified and counted. Nematodes were identified to species and sex, and counted. Total numbers in each sample were recorded on data sheets. All species of nematode, and all rotifers and tardigrades found in the sample were recorded. Data were entered in to Excel files, printed, and checked for errors.

rswo Comma delimited spreadsheet containing the rswo data described here rswo.csv 111441 1 0 \n column , " https://mcm.lternet.edu/sites/default/files/rswo.csv LOCATION LOCATION Name of area where measurement was made string Name of area where measurement was made DATE_TIME DATE_TIME Date on which sample was gathered date mm/dd/yyyy SAMPLE # SAMPLE # ID associated with transect, sampling location string ID associated with transect, sampling location TYPE OF ORGANISM TYPE OF ORGANISM Family associated with organism string Family associated with organism SPECIES SPECIES Species found in soil string Species found in soil SEX SEX Gender of organism (male vs. female) string Gender of organism (male vs. female) LIVE/DEAD/COMBINED LIVE/DEAD/COMBINED Survival Status (living, dead, or both) string Survival Status (living, dead, or both) ADULT/JUVENILE/COMBINED ADULT/JUVENILE/COMBINED Maturity (adult, juvenile, or both) string Maturity (adult, juvenile, or both) TOTAL (#/KG DRY SOIL) TOTAL (#/KG DRY SOIL) Number of organisms found in that category #/KG DRY SOIL real COMMENTS COMMENTS Helpful hints about the sample string Helpful hints about the sample FILE NAME FILE NAME Name of file in which data was stored string Name of file in which data was stored