uid=MCM,o=EDI,dc=edirepository,dc=org all public read upper_ponds Daryl Moorhead

Department of Earth, Ecology and Environmental Sciences Toledo OH 43606 US

(419) 530-2017 (419) 530-4421 daryl.moorhead@utoledo.edu Diana Wall

Johnson Hall 107 Fort Collins CO 80523 US

Diana.Wall@colostate.edu http://wp.natsci.colostate.edu/walllab/ https://orcid.org/0000-0002-9466-5235 Ross Virginia

Hinman Box 6182 Hanover NH 03755 US

(603) 646-0192 ross.a.virginia@dartmouth.edu http://sites.dartmouth.edu/ravirginia/ https://orcid.org/0000-0002-0890-0981 McMurdo Dry Valleys LTER http://mcmlter.org/ John "Jeb" Barrett jebarre@vt.edu https://www.biol.vt.edu/faculty/barrett/index.html https://orcid.org/0000-0002-7610-0505 associated researcher Dorota Porazinska

1JSCB Jennie Caruthers Biotech Bldg Boulder CO 80303 US

dorota.porazinska@colorado.edu lab crew Inigo San Gil

Department of Biology, MSC03 2020 University of New Mexico Albuquerque NM 87131 US

(505) 277-2625 (505) 277-2541 isangil@lternet.edu data manager Michelle Haddix

Natural Resource Ecology Laboratory Fort Collins CO 80523 US

michelle.haddix@colostate.edu lab crew 2014-01-01 English

In January 2001, we surveyed streams and ponds above 300 m asl in Taylor Valley, South Victoria Land, Antarctica. One pond was examined in detail. Organic materials covered nearly 100% of the adjacent soil to 5-20 m from the shore, with intermittent patches to 80 m. Organic matter averaged 257 gC/m2, and totaled 1388 kg organic C on the soil around the pond. Soil moisture content (0.56 to 12.41%) decreased with distance from shore, whereas pH (7.8 to 10.8) increased with distance. Electrical conductivity was lowest in the soils less than 10 m from the pond (416 +- 94 uS/cm). Mineral soil organic C and total N concentrations were greatest between 10-30 m from the edgeof the pond (1.21 +- 0.37 and 0.13 +- 0.05 mg/g soil, respectively). Soil invertebrates were present in only 50% of samples and included tardigrades, rotifers and two nematodes, Scottnema lindsayae and Plectus antarcticus. A non-parametric, discriminant function analysis based on soil moisture, soil organic carbon and electrical conductivitycorrectly predicted 87.0% of sites that had invertebrates and 70.8% of sites for which invertebrates were absent. Tardigrades, rotifers and Plectus were found only in the wettest soils nearest the pond whereas Scottnema was restricted to drier soils farther from shore. Other ponds and streams also showed substantial accumulations of organic matter, suggesting that upland wetlands serve as resource islands in these polar deserts that provide a source of organic matter to nearby soils.

carbon nematodes ph rotifers soil carbon soil moisture soil nitrogen soil ph soil water content LTER Controlled Vocabulary Antarctica carbon Eudorylaimus LTER nematodes pH Plectus rotifers Scottnema soil soil carbon soil moisture soil nitrogen soil organic C tardigrade water content Station Keywords population dynamics LTER Core Areas  Since the attribute table for this dataset is very large, the user must construct the actual column       name.  The FIRST letter represents the species, and the following letters represent the life stage/sex/sum type.  The nermatode       species codes are:              S: Scottnema lindsayae        E: Eudorylaimus spp.        P: Plectus spp.                For example, in the attribute table,  "(code)ML" has the description "The total number of living male (species) adult nematodes extracted       from the soil sample in number of organisms per kg soil oven dry weight equivalent. In this case, a column name        called "SML" would be  "The total number of living male Scottnema lindsayae adult nematodes..."   Preserved nematode samples are stored in the NREL Preserved Nematode Collection in room 226, College of Natural Resources, Colorado State University. Soils are stored in the Environmental Measurements Laboratory at Dartmouth College.

This data package is released under the Creative Commons Attribution 4.0 International License (CC BY 4.0; http://creativecommons.org/licenses/by/4.0/), which allows consumers (hereinafter referred to as “Data Users”) to freely reuse, redistribute, transform, or build on this work (even commercially) so long as appropriate credit is provided. Accordingly, Data Users are required to properly cite this data package in any publications or in the metadata of any derived products that result from its use (in whole or in part). A recommended citation is provided on the summary metadata page associated with this data package in the McMurdo Dry Valleys LTER Data Catalog (https://mcmlter.org/data), and a generic citation may be found on the summary metadata page in the repository where this data package was obtained. When these data contribute significantly to the contents of a publication, Data Users must also acknowledge that data were provided by the NSF-supported McMurdo Dry Valleys Long Term Ecological Research program (OPP-2224760). This data package has been released in the spirit of open scientific collaboration. Hence, Data Users are strongly encouraged to consider consultation, collaboration, and/or co-authorship (as appropriate) with the data package creator(s). Data Users should be aware these data may be actively used by others for ongoing research; thus, coordination may be necessary to prevent duplicate publication. Data Users should also recognize that misinterpretation of data may occur if they are used outside the context of the original study. Hence, Data Users are urged to contact the data package creator(s) if they have any questions regarding methodology or results. While substantial efforts are made to ensure the accuracy of this data package (with all its components), complete accuracy cannot be guaranteed. Periodic updates to this data package may occur, and it is the responsibility of Data Users to check for new versions. This data package is made available “as is” and comes with no warranty of accuracy or fitness for use. The creator(s) of this data package and the repository where these data were obtained shall not be liable for any damages resulting from misinterpretation, use, or misuse of these data. Finally, as a professional courtesy, we kindly request Data Users notify the primary contact referenced in the metadata when these data are used in the production of any derivative work or publication. Notification should include an explanation of how the data were used, along with a digital copy of the derived product(s). Thank you.

https://mcm.lternet.edu/content/upper-pond-nematode-survey-experiment  4 transects around single pond (Highland Pond) running N, S, E and W. The zones in this study refer to foliar mat cover (and were correlated with distance from the pond). Zone 1 was 0-10m from pond, zone 2 10-30m, zone 3 ~20-80m and zone 4 >80m. Three reps were taken from each “zone” in each transect for a total of 48 samples. 162.307296752930 162.316223144531 -77.724754333496 -77.726249694824 2001-01-01 2001-01-31 This file contains data compiled by Ed Khun, Andy Parsons and Jeb Barrett. The final data QA/QC and analysis were performed by Jeb Barrett. McMurdo Dry Valleys LTER http://mcmlter.org/ McMurdo Dry Valleys LTER http://mcmlter.org/ McMurdo Dry Valleys LTER

The mass and extent of organic matter cover on soils adjacent to Higland Pond were examined with line transects extending from the approximate center of the pond to a distance of 90 m beyond the shoreline. Eight line transects were established, approximately oriented in N, NE, E, SE, S, SW, W, and NW directions. An estimate of organic matter cover on soil surfaces was determined as the fraction of the distance over which organic material intercepted each line. Organic matter samples were collected at intervals along each transect at ca. 50 cm intervals within continuous cover and where present within areas of patchy cover. Collections were made with a 0.83 cm diameter steel corer, thrust through the organic layer into the underlying mineral soil. Organic matter samples were returned to the Crary Laboratory facility, McMurdo Station, Antarctica for mass determinations. Samples were placed in a drying oven at 60degC for 24 hours. Dry weights were recorded and samples then were placed in a muffle furnace for 6 hours at a maximum temperature of 500degC. Ash weights were recorded and ash-free dry weights calculated for all samples. These data then were used to estimate the amount of organic matter on soil surfaces associated with upland wetlands. All estimates of ash-free dry mass were converted to units of organic carbon, assuming a content of 50% carbon by weight. Mineral soil samples (n = 48) were collected around Highland Pond along transects established in each of the 4 off-cardinal directions. Samples were collected in 4 zones classified by extent of surface organic mat cover and appearance (foliar or granular mats). Zone 1 consisted of soils with foliar mats covering 100% of the surface and extended to 10 m from the edge of the pond. Zone 2 had granular mats covering greater than 30% of the soil surface and extended about 10-30 m from the edge of the pond. Zone 3 had intermittent mats covering less than 20% of the soil surface and extended 20-80 m from the pond. Zone 4 had no visible mats and extended greater than 80 m from the edge of the pond. Each sample consisted of approximately 1 kg of soil collected from the top 10 cm of the mineral soil profile with pre-sterilized Nasco trowels. Three samples were collected from each zone along each of the 4 transects, for a total of 48 samples. Sampling excluded surface organic material. Samples were sealed in Whirl-Pak polyethylene bags and transported to Crary Laboratory at McMurdo Station for analyses. At Crary Laboratory, mineral soils were sieved to remove rock fragments greater than 2 mm. Subsamples were oven-dried at 50degC for 48 h to determine gravimetric soil moisture content. Invertebrates were extracted from approximately 100 g soil using the modified sugar centrifugation technique described by Freckman and Virginia (1993). Extraction of invertebrates was completed within 48 h of collection. Species identification and life stage were determined and enumerated using light microscopy. Soil salinity was determined from the electrical conductivity of a 1:5 soil solution of sieved soil in de-ionized water. Electrical conductivity was measured using a Corning 311 conductivity meter, temperature-calibrated with a 0.01 M KCl solution. Soil pH was determined from a saturated paste (1:2 soil in de-ionized water) with an Orion model 720A pH meter. A subsample of each soil sample was ground in a ball mill and acidified with 50% HCl to remove carbonates. Approximately 75 mg of both the acidified and un-acidified soil were analyzed with a Carlo Erba 1500 elemental analyzer (Milan, Italy) to determine total carbon and nitrogen, and organic carbon content. Carbonate content of the soil was calculated as the difference between total carbon and organic carbon by comparing results from the acidified and un-acidified samples.

2000-2001 Field Notes Log: This file was created by Rebecca Lavier.  Data were entered by Rebecca Lavier on 12 February 2001. These data were proofed by Pilar Tillberg and Rebecca Lavier on 14 February 2001. [Pilar Tillberg 14 Feb 01] Calculations for # nematodes per kg soil were done by Rebecca Lavier on 15 February 2001.  [Rebecca Lavier 15 Feb 01]. Soil Moisture calculated as the (g of water/ g dry soil) *100 by M L Haddix 9/26/14 Comments: Data were collected from the Upper Pond site in Antarctica.  Data were collected on 22 January 2001.  Nematodes were extracted on 24 January 2001.  Samples were counted by Dorota Porazinska on 25 January and 26 January 2001. [Rebecca Lavier 12 Feb. 01].

SOILS_UPPERPONDS details about the data-containing spreadsheet, such as units and definitions. SOILS_UPPERPONDS.csv 10520 28 1 \n column , " https://mcm.lternet.edu/sites/default/files/data/SOILS_UPPERPONDS.csv DATASET_CODE DATASET_CODE Unique identifier for the table in the MCM LTER database string Unique identifier for the table in the MCM LTER database OBSERVATION Observation Sample number (1-48) string Sample number (1-48) TRANSECT Transect The transect from where the soil sample was collected. string north North Transect south South Transect east East Transect west West Transect ZONE ZONE The zone number that serves to ID the exact plot location where data was collected string 1 Zone ID 1 2 Zone ID 2 3 Zone ID 3 4 Zone ID 4 REP REP The number (1-3) assigned to replicate samples collected from each zone along a transect string 1 First Replicate 2 Second Replicate 3 Third Replicate DATE_TIME DATE_TIME Date and time when sample was aquired date MM/DD/YYYY TN TN Total soil nitrogen (% N by wt.), the nitrogen content of the unacidified soil dimensionless real 0 Null None given SOC SOC Soil organic carbon (% C by wt.), the carbon content of acidified soil dimensionless real 0 Null None given CN C:N The ratio of soil organic carbon to total nitrogen dimensionless real 0 Null None given SOIL_WATER_CONTENT soil water content The gravimetric soil water content percent g/g real 0 100 Null None given PH pH The opposite loge the hydrogen ion concentration of a 1:2 soil-water paste pH real 0 Null None given EC Electical Conductivity The electrical conductivity of a 1:5 soil-water dilution uS/cm real 0 Null None given SML SML The total number of living male Scottmema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given SMD SMD The total number of dead male Scottmema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given SFL SFL The total number of living female Scottmema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given SFD SFD The total number of dead female Scottmema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given SJL SJL The total number of living juvenile Scottmema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given SJD SJD The total number of dead juvenile Scottmema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given SA SA The total number of living and dead Scottmema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given SJ SJ The total number of living and dead Scottmema lindsayae juvenile nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given STL STL total number of living Scottmema lindsayae (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given STD STD The total number of dead Scottmema lindsayae (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given STLD STLD The total number of live + dead Scottmema lindsayae (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given EML EML The total number of living male Eudorylaimus adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given EMD EMD The total number of dead male Eudorylaimus adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given EFL EFL The total number of living female Eudorylaimus adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given EFD EFD The total number of dead female Eudorylaimus adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given EJL EJL The total number of living juvenile Eudorylaimus adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given EJD EJD The total number of dead juvenile Eudorylaimus adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given EA EA The total number of living and dead Eudorylaimus adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given EJ EJ The total number of living and dead Eudorylaimus juvenile nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given ETL ETL total number of living Eudorylaimus (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given ETD ETD The total number of dead Eudorylaimus (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given ETLD ETLD The total number of live + dead Eudorylaimus (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given PFL PFL The total number of living female Plectus adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given PFD PFD The total number of dead female Plectus adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given PJL PJL The total number of living juvenile Plectus adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given PJD PJD The total number of dead juvenile Plectus adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given PA PA The total number of living and dead Plectus adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given PJ PJ The total number of living and dead Plectus juvenile nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given PTL PTL total number of living Plectus (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given PTD PTD The total number of dead Plectus (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given PTLD PTLD The total number of live + dead Plectus (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent # kg/soil real 0 Null None given TOTAL_ROTIFERS total rotifers total number of rotifers per kg soil oven dry weight equivalent present in a sample # kg/soil real 0 Null None given TOTAL_TARDIGRADES total tardigrades total number of tardigrades per kg soil oven dry weight equivalent present in a sample # kg/soil real 0 Null None given LIVENEMS Total Live (Livenems) The total number of living nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent (#/kg soil). # kg/soil real 0 Null None given DEADNEMS Total Dead (Deadnems) The total number of dead nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent (#/kg soil). # kg/soil real 0 Null None given TOTALNEMS Total (Totalnems) The total number of living and dead nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent (#/kg soil). # kg/soil real 0 Null None given TAXA Other Organisms Taxa The total number of metazoan taxa extracted from the soil sample dimensionless real COMMENTS COMMENTS Comments string Comments pH