uid=MCM,o=EDI,dc=edirepository,dc=org all public read Elev_tran Byron Adams byron_adams@byu.edu https://biology.byu.edu/adams-lab https://orcid.org/0000-0002-7815-3352 John "Jeb" Barrett jebarre@vt.edu https://www.biol.vt.edu/faculty/barrett/index.html https://orcid.org/0000-0002-7610-0505 Diana Wall

Johnson Hall 107 Fort Collins CO 80523 US

Diana.Wall@colostate.edu http://wp.natsci.colostate.edu/walllab/ https://orcid.org/0000-0002-9466-5235 Ross Virginia

Hinman Box 6182 Hanover NH 03755 US

(603) 646-0192 ross.a.virginia@dartmouth.edu http://sites.dartmouth.edu/ravirginia/ https://orcid.org/0000-0002-0890-0981 McMurdo Dry Valleys LTER http://mcmlter.org/ Abigail Borgmeier ab6240@student.byu.edu https://orcid.org/0009-0003-7244-6255 field technician Renée Brown rfbrown@unm.edu https://orcid.org/0000-0002-4986-7663 data manager Jesse Jorna jjgjorna@outlook.com https://orcid.org/0000-0001-8826-9191 former field crew Michelle Haddix

Natural Resource Ecology Laboratory Fort Collins CO 80523 US

michelle.haddix@colostate.edu former lab crew Martijn Vandegehuchte

Natural Resource Ecology Laboratory. 1231 East Drive Fort Collins CO 80523 US

(970) 491-2162 (970) 491-1965 vandegehuchte@colostate.edu former field crew Ashley Shaw

Colorado State University Fort Collins CO 80523 US

(970) 491-7802 Elizabeth.Shaw@colorado.edu former lab crew Sabrina Saurey

US

sabrinasaurey@gmail.com former lab crew Zachary Sylvain

Department of Biology Fort Collins CO 80523 US

zachary.sylvain@colostate.edu former lab crew Jeremy Whiting former lab crew Karen Seaver former lab crew Dorota Porazinska

1JSCB Jennie Caruthers Biotech Bldg Boulder CO 80303 US

dorota.porazinska@colorado.edu former lab crew Ed Ayres

1685 38th Street, suite 100 Boulder CO 80301 US

720 836 2402 eayres@neoninc.org former lab crew Breana Simmons bsimmons@ega.edu former lab crew Emma Broos

atural Resource Ecology Laboratory, Colorado State University Fort Collins CO 80523 US

emma@nrel.colostate.edu former lab crew John Chaston

College of Life Sciences Provo UT 84602 US

(801) 422-4553 john_chaston@byu.edu http://chastonlab.byu.edu/ former lab crew Holley Zadeh former lab crew Steve Blecker

usgs reno NV 89557 US

sblecker@usgs.gov former lab crew Claire Ojima former lab crew Inigo San Gil

Department of Biology, MSC03 2020 University of New Mexico Albuquerque NM 87131 US

(505) 277-2625 (505) 277-2541 isangil@lternet.edu former data manager 2025-04-30 English

Investigation of the effect of elevation and topography on soil biota and soil properties was part of the McMurdo Dry Valleys Long Term Ecological Research (LTER) project. The number of soil organisms (nematodes, rotifers and tardigrades), divided by species, sex and maturity was monitored at 3 elevations, initially in Taylor Valley (1993) then Garwood and Miers Valleys (2012) in order to accomplish this.

ammonia chlorophyll a conductivity elevation microbial biomass microbial nitrogen nematodes ph rotifers soil carbon soil chemistry soil moisture soil ph soil water content topography total nitrogen total organic carbon transects LTER Controlled Vocabulary ammonia Antarctica chlopophyl-A conductivity elevation Eudorylaimus LTER McMurdo Dry Valleys microbial biomass nematodes nitrate pH Plectus rotifers Scottnema soil soil carbon soil moisture soil nitrogen soil organic C tardigrade topography total nitrogen transect water content Station Keywords population dynamics LTER Core Areas  Since the attribute table for this dataset is very large, the user must construct the actual column name.  The FIRST letter represents the species, and the following letters represent the life stage/sex/sum type.  The nermatode  species codes are:              S: Scottnema lindsayae        E: Eudorylaimus spp.        P: Plectus spp.                For example, in the attribute table,  "(code)ML" has the description "The total number of living male (species) adult nematodes extracted       from the soil sample in number of organisms per kg soil oven dry weight equivalent. In this case, a column name        called "SML" would be  "The total number of living male Scottnema lindsayae adult nematodes..."       In the data,         ND = no data; sample not taken       BD = below detection limit             

This data package is released under the Creative Commons Attribution 4.0 International License (CC BY 4.0; http://creativecommons.org/licenses/by/4.0/), which allows consumers (hereinafter referred to as “Data Users”) to freely reuse, redistribute, transform, or build on this work (even commercially) so long as appropriate credit is provided. Accordingly, Data Users are required to properly cite this data package in any publications or in the metadata of any derived products that result from its use (in whole or in part). A recommended citation is provided on the summary metadata page associated with this data package in the McMurdo Dry Valleys LTER Data Catalog (https://mcmlter.org/data), and a generic citation may be found on the summary metadata page in the repository where this data package was obtained. When these data contribute significantly to the contents of a publication, Data Users must also acknowledge that data were provided by the NSF-supported McMurdo Dry Valleys Long Term Ecological Research program (OPP-2224760). This data package has been released in the spirit of open scientific collaboration. Hence, Data Users are strongly encouraged to consider consultation, collaboration, and/or co-authorship (as appropriate) with the data package creator(s). Data Users should be aware these data may be actively used by others for ongoing research; thus, coordination may be necessary to prevent duplicate publication. Data Users should also recognize that misinterpretation of data may occur if they are used outside the context of the original study. Hence, Data Users are urged to contact the data package creator(s) if they have any questions regarding methodology or results. While substantial efforts are made to ensure the accuracy of this data package (with all its components), complete accuracy cannot be guaranteed. Periodic updates to this data package may occur, and it is the responsibility of Data Users to check for new versions. This data package is made available “as is” and comes with no warranty of accuracy or fitness for use. The creator(s) of this data package and the repository where these data were obtained shall not be liable for any damages resulting from misinterpretation, use, or misuse of these data. Finally, as a professional courtesy, we kindly request Data Users notify the primary contact referenced in the metadata when these data are used in the production of any derivative work or publication. Notification should include an explanation of how the data were used, along with a digital copy of the derived product(s). Thank you.

https://mcm.lternet.edu/content/soil-elevational-transect-experiment Plot corresponding to the Elevation Transect. This is the plot A by the south side of Lake Hoare 162.889373779297 162.889373779297 -77.632812500000 -77.632812500000 Plot corresponding to the Elevation Transect. This is the plot B by the south side of Lake Hoare 162.889373779297 162.889373779297 -77.635581970215 -77.635581970215 Plot corresponding to the Elevation Transect. This is the plot C by the south side of Lake Hoare 162.886932373047 162.886932373047 -77.637611389160 -77.637611389160 The plot corresponds to the Elevation Transects at Garwood Valley, specifically the A1 Treatment Plot 163.899291992188 163.899291992188 -78.021018981934 -78.021018981934 The plot corresponds to the Elevation Transects experiments at Garwood Valley. Specifically, this corresponds to the A2 Treatment A1-plot 163.899566650391 163.899566650391 -78.020927429199 -78.020927429199 The plot corresponds to the Elevation Transects experiments at Garwood Valley. Specifically, this corresponds to the A3 Treatment A1 plot 163.899612426758 163.899612426758 -78.021049499512 -78.021049499512 This is the Elevation Transect plot at Miers, specifically the plot with the A1 Treatment    163.786712646484 163.786712646484 -78.094032287598 -78.094032287598 This is the Elevation Transect plot at Miers, specifically the plot with the A2 Treatment    163.786773681641 163.786773681641 -78.094200134277 -78.094200134277 This is the Elevation Transect plot at Miers, specifically the plot with the  A3 Treatment    163.787628173828 163.787628173828 -78.094207763672 -78.094207763672 Elevation Transects at the Miers Valleys, this corresponds to the B1 Treatment Plot 163.777709960938 163.777709960938 -78.092002868652 -78.092002868652 Elevation Transects at the Miers Valley, this corresponds to the B2 Treatment Plot 163.778152465820 163.778152465820 -78.091880798340 -78.091880798340 Elevation Transects at the Mires Valley, This is the B3 Treatment Plot 163.778427124023 163.778427124023 -78.092033386230 -78.092033386230 This is the Elevation Transect plot at Miers, specifically the plot with the  C1 Treatment    163.775085449219 163.775085449219 -78.088989257813 -78.088989257813 This is the Elevation Transect plot at Miers, specifically the plot with the  C2 Treatment  163.775634765625 163.775634765625 -78.089073181152 -78.089073181152 This is the Elevation Transect plot at Miers, specifically the plot with the  C3 Treatment    163.776565551758 163.776565551758 -78.088981628418 -78.088981628418 The plot corresponds to the Elevation Transects experiments at Garwood Valley. Specifically, this corresponds to the B1 Treatment  plot 163.886840820313 163.886840820313 -78.018379211426 -78.018379211426 The plot corresponds to the Elevation Transects experiments at Garwood Valley. Specifically, this corresponds to the B2 Treatment  plot 163.887268066406 163.887268066406 -78.018333435059 -78.018333435059 The plot corresponds to the Elevation Transects experiments at Garwood Valley. Specifically, this corresponds to the B3 Treatment  plot 163.886932373047 163.886932373047 -78.018463134766 -78.018463134766 The plot corresponds to the Elevation Transects experiments at Garwood Valley. Specifically, this corresponds to the C1 Treatment  plot 163.879333496094 163.879333496094 -78.017898559570 -78.017898559570 The plot corresponds to the Elevation Transects experiments at Garwood Valley. Specifically, this corresponds to the C2 Treatment  plot 163.879043579102 163.879043579102 -78.017799377441 -78.017799377441 The plot corresponds to the Elevation Transects experiments at Garwood Valley. Specifically, this corresponds to the C3 Treatment  plot 163.879837036133 163.879837036133 -78.017799377441 -78.017799377441 1993-12-19 2024-12-30 202503: IM Brown made several improvements to the dataset, including filling in missing soil moisture, pH, conductivity values and other values as needed, various corrections to sampling dates, standardization of sample IDs, removal of duplicate rows, fixed incorrect positions based on sample names, fixed issues with tardigrade counts misaligned starting in 2013.   This file contains archived data pulled from Nemadisk and the field season directories by Jeb Barrett.          The data page contains the raw data for nematode abundance in # of animals per kg oven dry weight equivalent.  McMurdo Dry Valleys LTER Information Manager im@mcmlter.org McMurdo Dry Valleys LTER http://mcmlter.org/ McMurdo Dry Valleys LTER

In 1993, three sites were chosen on generally flat benches of the glaciated slope near South Side Lake Hoare in Taylor Valley: A at 83 m above sea level, B at 121 m above sea level, and C at 188 m above sea level. In 2012 two further Valleys were added to this experiment: Garwood and Miers. As with Taylor Valley, three sites were chosen in each on generally flat benches of the glaciated slope: Garwood A at 369 m above sea level, B at 377 m above sea level, and C at 392 m above sea level. Miers A at 179 m above sea level, B at 220 m above sea level, and C at 290 m above sea level. A 20X 20 m grid was placed at each elevation, and soil samples were taken at the four corners of the grid, at the middle point of each side, and at the center of the square. A smaller 2 X 2 m grid was placed at the northwest corner of the larger grid, and samples were collected with the same scheme, though no second samples were taken from the overlapping corner. Soil samples were taken for organism enumeration and moisture content analysis as follows: Sampling bags were prepared with one sterile 'Whirlpak' bag and clean plastic scoop per sample. Samples were taken from within the 10 cm diameter circular area of each plot. The location of the sampling was recorded each year so that areas were not re-sampled. Using the plastic scoop, soil was collected to 10 cm depth. Very large rocks (greater than 20 mm diameter) were excluded from the sample. The soil was shoveled into the 'Whirlpak' bag until three quarters full (about 1.5 kg soil). The soil was mixed well in the bag, then the bag was closed tightly, expelling as much air as possible. The soil samples were stored in a cooler for transportation. On return to the laboratory (within 8 hours of sampling), the soils were stored at +5C until further processing. In the laboratory, soil samples were handled in a laminar flow hood to prevent contamination. The Whirlpak bags of soil were mixed thoroughly prior to opening. Approximately 200cm3 of soil was placed in a pre-weighed 800mL plastic beaker. Rocks greater than 3-4mm in diameter were removed from the sample. A sub-sample of approximately 50g was removed and placed in a pre-weighed aluminum dish, and weighed on a balance accurate to 0.01g. This sample was dried at 105C for 24 hours. The sample was removed, placed in desiccator to cool down, and re-weighed. These data were used to calculate water content of the soil, and to express data as numbers of soil organisms per unit dry weight of soil. The remaining soil in the plastic beaker was weighed. Cold tap water was added up to 650 mL. The soil suspension was stirred carefully (star stir or figure of 8) for 30 seconds, using a spatula. Immediately the liquid was poured into wet screens - a stack of 40 mesh on top of a 400 mesh. The screens were rinsed gently with ice cold tap water (from a wash bottle) through the top of the stack, keeping the screens at an angle as the water filtered through. The water was kept on ice at all times. The top screen was removed, and the lower screen rinsed top down, never directly on top of the soil, but at the top of the screen and from behind. The water was allowed to cascade down and carry the particles into the bottom wedge of the angled screen. The side of the screen was tapped gently to filter all the water through. The suspension was rinsed from the front and the back, keeping the screen at an angle and not allowing the water to overflow the edge of the screen. The soil particles were backwashed into a 50mL plastic centrifuge tube, tipping the screen into the funnel above the tube and rinsing the funnel gently. The suspension was centrifuged for five minutes at 1744 RPM. The liquid was decanted, leaving a few mL on top of the soil particles. The tube was filled with sucrose solution (454g sucrose per liter of tap water, kept refrigerated) up to 45mL. This was stirred gently with a spatula until the pellet was broken up and suspended. The suspension was centrifuged for one minute at 1744 RPM, decanted into a wet 500 mesh screen, rinsed well with ice cold tap water and backwashed into a centrifuge tube. Samples were refrigerated at 5C until counted. Samples were washed into a counting dish and examined under a microscope at x 10 or x20 magnification. Rotifers and tardigrades were identified and counted. Nematodes were identified to species and sex, and counted. Total numbers in each sample were recorded on data sheets. All species of nematode, and all rotifers and tardigrades found in the sample were recorded. Data were entered in to Excel files, printed, and checked for errors. For measurements of pH, an aqueous soil solution was made. DI-H2O was added to soil in a 1:2 soil:water ratio in a clean, DI- rinsed glass beaker (coarse fragments >2 mm were removed). The samples were stirred until thoroughly mixed (about 5-10 sec). After sitting to equilibrate for 2 h (all years up to 2002-03) or 10 min (all years 2004-05 and later), the samples were stirred again and a reading was taken with a pH meter. For measurements of electrical conductivity, DI-H2O was added to soil in a 1:5 soil:water ratio. The samples were stirred until thoroughly mixed (about 5-10 sec). After sitting to equilibrate for 2 h (all years up to 2002-03) or 10 min (all years 2004-05 and later), the samples were stirred again and a reading was taken with a conductivity meter. For extraction of chlorophyll from the soil, all procedures were carried out in the dark or very low irradiance to avoid degradation of the chlorophyll. The soil samples were mixed thoroughly in the vials, and a sample of approximately 5 g was weighed out in to a 50 mL plastic centrifuge tube with a screw-top cap. 10 mL of a 50:50 DMSO/90percent acetone solution was added to each sample and they were mixed thoroughly on a bench-top Vortex mixer for about 5 seconds. The vials were placed in a -4°C constant temperature room, in the dark, and left for 12-18 hours. Determination of chlorophyll a concentration was determined fluorometrically using a Turner model 111 fluorometer. A calibration using a known concentration of chlorophyll was carried out prior to sample analysis. The machine was blanked using a 50:50 DMSO/90percent acetone solution. A sample of approximately 4mL of the DMSO/acetone solution was taken from the top of the sample with a pipette, being careful not to get any soil particles in the solution. The sample was placed in a cuvette, into the fluorometer and the fluorescence was recorded. This was done fairly quickly in order to prevent light from breaking down the chlorophyll. This measurement is called Fo, the initial fluorescence. After taking this reading, 0.1 mL of 1N HCl was added directly to the cuvette and the cuvette was gently agitated. After 20 seconds, the fluorescence was re-measured. (During this step, the acid converts the chlorophyll to phaeophytin by releasing a magnesium ion in an acidic environment). This measurement is called Fa, the fluorescence after acidification. The solution was discarded in to a waste container, and the cuvette rinsed 3 times with DMSO/90percent acetone solution before proceeding with the next sample. For mineral N content, a 20 g subsample was extracted in 50 ml 2M KCl for 1 h and filtered through Whatman #42 filter paper. Extracts were frozen prior to analysis for NH4-N and NO2+NO3-N on a Lachat Quikchem. For total C and N, a subsample of soil was hand-ground using a sapphire mortar and pestle, from which a subsample was run on a Carlo Erba NA 1500 N Elemental Analyzer (Carlo Erba Instruments, Milan, Italy). For organic C, 1 mg of ground soil was acidified with 1 ml 6 N HCl and dried at 95°C for 48 h. A subsample was run on a Carlo Erba, and the value was corrected for the change in weight associated with acidification. Microbial C and N were measured using the chloroform fumigation extraction technique with a 1:2.5 ratio of soil and 0.5 M K2SO4. Approximately 20 g of soil from each sample was extracted in 50 ml of 0.5 M K2SO4. Extracts were shaken at 240 rpm for 45 minutes, then centrifuged at 15000 rpm for 15 min, and the supernatant brought to pH~2 with 0.5 ml 6N HCl. A duplicate 20-g subsample was placed in a vacuum desiccator and fumigated with ethanol-free chloroform for 120 h. After fumigation, soils were extracted as described above. All extracts were frozen prior to analysis on a Shimadzu TOC analyzer for DOC and DIN. Season Notes 2002-2003 File log: This file was created on 15 Jan 2003 by Steve Blecker. Extraction weight entered at this time [Steve Blecker 15 Jan 03].  Nema data entered on 21 Jan 03 by Steve Blecker [Steve Blecker 21 Jan 03]. Calculation done by Jeb Barrett 22 Jan 03. Raw data checked and corrected [Emma Broos 4 March 03]. Numbers per kg dry soil changed by M L Haddix using formula 1000*(#individuals/(extraction mass*(mass of dry soil/mass of soil used for moisture calc))) 7/22/13 Soil Moisture calculated as the (g of water/ g dry soil) *100 by M L Haddix 9/06/13 File was abbreviated for website only counts in #/kg dry soil was left along with log, 7/22/13 MLHaddix 2004-2005 File log:  Soil data was entered, and log was created, on 14 Jan 2005 by Holley Zadeh. Data verified by Holley Zadeh on 23 Jan 05. Comments: These data are for soils collected by Diana Wall, John Chaston, and Holley Zadeh. Emma Broos and Claire Ojima kept track of sample locations, logged them in their field books, and made sure sample bags were correct. See map of sampling locations in drawing box below. To note: A1 was not mixed in the field, and we forgot to mix it before sampling. Also, C3 was counted, but after counting was dropped, so we do not have a sample to preserve. Samples were extracted at the Crary lab on 12 Jan 2005 as follows: *Holley Zadeh weighed soil under the laminar flow hood and recorded weights *Diana Wall sieved soil at the sink *Byron Adams spun samples in the centrifuge *John Chaston and Emma Broos counted specimens at the microscope. They were counted on the 13th and 14th of January 2005. Numbers per kg dry soil changed by M L Haddix using formula 1000*(#individuals/(extraction mass*(mass of dry soil/mass of soil used for moisture calc))) 7/22/13 Soil Moisture calculated as the (g of water/ g dry soil) *100 by M L Haddix 9/06/13 Related Files: ET_worms.xls; DNAExtr0405.xls File was abbreviated for website only counts in #/kg dry soil was left along with log, 7/22/13 MLHaddix 2006-2007 Comments: Soil samples for faunal extraction and chlorophyll a were collected by Ed Ayres, Breana Simmons, Dorota Porazinska on 18 Jan 2007.  Local scale sampling of spatial variation was conducted at the '6' location of each ET plot.  Soil samples were collected ~10 cm from the nail at 1 o'clock (if 12 o'clock is upslope).  Take extra nails for small scale sampling next time (at least 9). Soil was extracted on 20 Jan 2007. Nematodes preserved 23 Jan 2007.  Worms counted by Dorota Porazinska Data entered on 22 Jan 2007 by Ed Ayres.  Data was proofed by Dorota Porazinska. Fauna were extracted by sugar floatation.  Raw data were converted by Ed Ayres using (# individuals/(fresh soil wt extracted/((soil moisture/100)+1)))*1000. Numbers per kg dry soil changed by M L Haddix using formula 1000*(#individuals/(extraction mass*(mass of dry soil/mass of soil used for moisture calc))) 7/03/13 Soil Moisture calculated as the (g of water/ g dry soil) *100 by M L Haddix 9/06/13 Related Files: ET_labels.doc, ET_soils.xls, ET_COC.xls 2008-2009 LOG: This file created on 12/29/08 by Karen "rainbow Sprinkles" Seaver. COMMENTS: Samples were collected from the LTER core study, the Elevational Transect by Breana Simmons, Karen Seaver? and ??. Note: A sulfur smell was noted at the C elevation? Nematodes were extracted by     on Data entered by Karen Seaver and Uffe Nielsen on 12/28/08  Data checked by Karen Seaver and her electronic boyfriend Alex on 12/29/08. RELATED FILES: Bre - microbial biomass if we get it, ? ET_LABEL.XLS ET_SOILS.xls LTM_ET_COC.xls Dryvalleydisturbance_08_09.xls (In Reports Folder)  GPS coordinates Numbers per kg dry soil changed by M L Haddix using formula 1000*(#individuals/(extraction mass*(mass of dry soil/mass of soil used for moisture calc))) 7/11/13 Soil Moisture calculated as the (g of water/ g dry soil) *100 by M L Haddix 9/06/13 File was abbreviated for website only counts in #/kg dry soil was left along with log, 5/01/14 MLHaddix Inigo San Gil revised data, updated database records and metadata 2011-2012 Garwood LOG: This file created on 1/13/12 by Zachary Sylvain. Data entered by Zachary Sylvain. Data checked by Zachary Sylvain. Calculations by Zachary Sylvain using the formula: (# individuals / dry soil) * 1000 Numbers per kg dry soil adjusted by Martijn L Vandegehuchte using formula 1000*(#individuals/(extraction mass*(mass of dry soil/mass of soil used for moisture calc))) Soil Moisture calculated as the (g of water/ g dry soil) *100 by M L Haddix 11/26/13 ET LOG: This file created on 1/13/12 by Zachary Sylvain. Data entered by Jeremy Whiting. Data checked by Jeremy Whiting. Calculations by Zachary Sylvain using the formula: (# individuals / dry soil) * 1000 The majority of the worms were counted on Jan 6. Some worms were counted on the 5th and 7th as well. Numbers per kg dry soil adjusted by Martijn L Vandegehuchte using formula 1000*(#individuals/(extraction mass*(mass of dry soil/mass of soil used for moisture calc))) Soil Moisture calculated as the (g of water/ g dry soil) *100 by M L Haddix 9/06/13 Miers LOG: This file created on 1/11/12 by Zachary Sylvain. Data entered by Jeremy Whiting. Data checked by Jeremy Whiting. Calculations by Zachary Sylvain using the formula: (# individuals / dry soil) * 1000 C Transect      Polygon C3: 26m x 16m      Spike: S78.08898, E163.77657      Polygon C2: 14.5m x 12.3m      Spike: S78.08907, E163.77563      Polygon C1: 15.5m x 15.5m      Spike: S78.08899, E163.77509 B Transect      Polygon B1 Spike: S78.09200, E163.77771      Polygon B2 Spike: S78.09188, E163.77815      Polygon B3 Spike: S78.09203, E163.77843 A Transect      Polygon A1: 12.6m x 14.2m      Spike: S78.09403, E163.78671      Polygon A2: 12.7m x 12m      Spike: S78.09420, E163.78677      Polygon A3: 9.7m x 9.3m      Spike: S78.09421, E163.78763 MC3 sampled in a line downslope toward stream MC2 sampled down toward lake MC2 sampled upslope MB1 sampled toward northern edge of lake MB2 sampled downslope toward MB3 MB3 sampled toward northern edge of lake MA1 sampled downslope MA2 sampled downslope MA3 sampled upslope Numbers per kg dry soil adjusted by Martijn Vandegehuchte using formula 1000*(#individuals/(extraction mass*(mass of dry soil/mass of soil used for moisture calc))) Soil Moisture calculated as the (g of water/ g dry soil) *100 by M L Haddix 11/26/13 2012-2013 ET LOG: This file created on 20 January 2013 by Ashley Shaw. Data entered by Ashley Shaw. Data checked by Sabrina Saurey. Calculations by Martijn Vandegehuchte using the formula: 1000*(#individuals/(extraction mass*(mass of dry soil/mass of soil used for moisture calc))) Soil Moisture calculated as the (g of water/ g dry soil) *100 by M L Haddix 9/06/13 Garwood LOG: This file created on Jan 18 by Sabrina Saurey. Data entered by Sabrina Saurey. Data checked by Ashley Shaw. Calculations by Martijn Vandegehuchte using the formula: 1000*(#individuals/(extraction mass*(mass of dry soil/mass of soil used for moisture calc))) Soil Moisture calculated as the (g of water/ g dry soil) *100 by M L Haddix 11/13/13 Miers LOG: This file created on Jan 19 by Sabrina Saurey. Data entered by Sabrina Saurey. Data checked by Ashley Shaw on 20 January 2013 Calculations by Martijn Vandegehuchte using the formula: 1000*(#individuals/(extraction mass*(mass of dry soil/mass of soil used for moisture calc))) Soil Moisture calculated as the (g of water/ g dry soil) *100 by M L Haddix 11/13/13

SOILS_ELEVATION_TRN mcmlter-soil-et-20250430.csv 258314 1 1 \n column , " https://mcm.lternet.edu/sites/default/files/data/mcmlter-soil-et-20250430.csv DATASET_CODE Dataset Code Unique identifier for the table in the MCM LTER database. string Unique identifier for the table in the MCM LTER database. VALLEY Valley A sorting header indicating the Valley (Taylor, Miers, Garwood) from which the sample was collected. string A sorting header indicating the Valley (Taylor, Miers, Garwood) from which the sample was collected. SAMPLE_DATE Sample date The date the soil sample was collected from the field. date mm/dd/yyyy SAMPLE_ID Sample ID The plot-position identifier for each sample. string The plot-position identifier for each sample. ELEVATION Elevation The elevation of the plot from where the sample was collected. Taylor: A at 83 m above sea level, B at 121 m above sea level, C at 188 m above sea level. Garwood: A at 369 m above sea level, B at 377 m above sea level, and C at 392 m above sea level. Miers: A at 179 m above sea level, B at 220 m above sea level, and C at 290 m above sea level. string The elevation of the plot from where the sample was collected. Taylor: A at 83 m above sea level, B at 121 m above sea level, C at 188 m above sea level. Garwood: A at 369 m above sea level, B at 377 m above sea level, and C at 392 m above sea level. Miers: A at 179 m above sea level, B at 220 m above sea level, and C at 290 m above sea level. POSITION Position The position within a plot where the sample was collected. string The position within a plot where the sample was collected. SML Scottnema Male Live The total number of living male Scottnema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given SMD Scottnema Male Dead The total number of dead male Scottnema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given SFL Scottnema Female Live The total number of living female Scottnema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given SFD Scottnema Female Dead The total number of dead female Scottnema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given SJL Scottnema Juvenile Live The total number of living juvenile Scottnema lindsayae nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given SJD Scottnema Juvenile Dead The total number of dead juvenile Scottnema lindsayae nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given SA Scottnema Adult The total number of living and dead Scottnema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given SJ Scottnema Juvenile The total number of living and dead Scottnema lindsayae juvenile nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given STL Scottnema Total Live The total number of living Scottnema lindsayae (juvenile and adult) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given STD Scottnema Total Dead The total number of dead Scottnema lindsayae (juvenile and adult) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given STLD Total Scottnema Live and Dead The total number of live + dead Scottnema lindsayae (juvenile and adult) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given EML Eudorylaimus Male Live The total number of living male Eudorylaimus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given EMD Eudorylaimus Male Dead The total number of dead male Eudorylaimus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given EFL Eudorylaimus Female Live The total number of living female Eudorylaimus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given EFD Eudorylaimus Female Dead The total number of dead female Eudorylaimus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given EJL Eudorylaimus Juvenile Live The total number of living juvenile Eudorylaimus spp. nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given EJD Eudorylaimus Juvenile Dead The total number of dead juvenile Eudorylaimus spp. nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given EA Eudorylaimus Adult The total number of living and dead Eudorylaimus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given EJ Eudorylaimus Juvenile The total number of living and dead Eudorylaimus spp. juvenile nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given ETL Total Eudorylaimus Live Total number of living Eudorylaimus spp. (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given ETD Total Eudorylaimus Dead The total number of dead Eudorylaimus spp. (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given ETLD Total Eudorylaimus Live and Dead The total number of live + dead Eudorylaimus spp. (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given PFL Plectus Female Live The total number of living female Plectus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given PFD Plectus Female Dead The total number of dead female Plectus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given PJL Plectus Juvenile Live The total number of living juvenile Plectus spp. nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given PJD Plectus Juvenile Dead The total number of dead juvenile Plectus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given PA Plectus Adult The total number of living and dead Plectus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given PJ Plectus Juvenile The total number of living and dead Plectus spp. juvenile nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given PTL Plectus Total Live Total number of living Plectus spp. (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given PTD Plectus Total Dead The total number of dead Plectus spp. (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given PTLD Plectus Total Live and Dead The total number of live + dead Plectus spp. (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given TOTAL_LIVE Total Living Nematodes The total number of living nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given TOTAL_DEAD Total Dead Nematodes The total number of dead nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given TOTAL Total The total number of living and dead nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. # kg/soil real 0 Null None given TOTAL_ROTIFERS Total Rotifers Total number of rotifers per kg soil oven dry weight equivalent present in a sample. # kg/soil real 0 Null None given TOTAL_TARDIGRADES Total Tardigrades Total number of tardigrades per kg soil oven dry weight equivalent present in a sample. # kg/soil real 0 Null None given SOIL_WATER_CONTENT Soil Water Content The gravimetric soil water content. percent g/g real 0 100 Null Missing CONDUCTIVITY Conductivity The electrical conductivity in microSiemens/cm of a 1:5 soil:deionized water solution. microSiemens/cm real Null None given PH pH The opposite log of the hydrogen ion concentration of a 1:2 soil-water paste. dimensionless real 0 Null None given TN Total Nitrogen Total nitrogen, the nitrogen content of the unacidified soil. mg N/g soil real 0 Null None given SOC Soil Organic Carbon Soil organic carbon, the carbon content of acidified soil. mg C/g soil real 0 Null None given SIC Soil Inorganic Carbon Soil inorganic carbon, the carbon content of unacidified soil minus the carbon content of the acidified soil. mg C/g soil real 0 Null None given NH4_N Ammonium Ammonium concentration found using Lachat. microgram/g soil 0.1 real 0 2000 Null None given NO3_N Nitrate Nitrate concentration found using Lachat. microgram/g soil 0.1 real 0 2000 Null None given CHL_A Chlorophyll a The chlorophyll a content of the sample weight in micrograms per gram of soil oven dry weight equivalent. ug/g real Null None given MICROBIAL_C Microbial Carbon Biomass Microbial biomass carbon microgram C/g soil 0.1 real 0 2000 Null None given MICROBIAL_N Microbial Nitrogen Biomass Microbial biomass nitrogen microgram N/g soil 0.1 real 0 2000 Null None given COMMENTS Comments Comments about the sample. string Comments about the sample.