uid=MCM,o=EDI,dc=edirepository,dc=org all public read LIMNO_PHYTO_PROFILE Jeff Patriarche

334 Leon Johnson Hall Bozeman MT 59717 US

j.patriarche@gmail.com John Priscu jpriscu@montana.edu https://orcid.org/0000-0001-5807-6364 Cristina Takacs-Vesbach cvesbach@unm.edu http://www.vesbachlab.org https://orcid.org/0000-0002-5535-2201 Luke Winslow 608-821-3914 lwinslow@usgs.gov http://cida.usgs.gov/people/lwinslow.html Krista Myers Heather Buelow

University of New Mexico Albuquerque NM 87131 US

hnbuelow@gmail.com https://scholar.google.com/citations?user=Ut_MuNcAAAAJ&hl=en Rachael Morgan-Kiss morganr2@miamioh.edu https://photopsychrophile.weebly.com https://orcid.org/0000-0003-4783-5358 Peter Doran pdoran@lsu.edu https://www.lsu.edu/science/geology/people/faculty/doran.php https://orcid.org/0000-0002-3774-2847 McMurdo Dry Valleys LTER http://mcmlter.org/ Renée Brown rfbrown@unm.edu https://orcid.org/0000-0002-4986-7663 data manager 2019-10-18 English

Lake Bonney (McMurdo Dry Valleys, east Antarctica) represents a year-round refugia for life adapted to extreme conditions. Lake level has risen by more than 3 m since 2004, but impacts of rapid lake level rise on phytoplankton community structure is also poorly understood. From 2004 to 2015, in concert with the summer limnological samplings, an in situ submersible spectrofluorometer (bbe FluoroProbe) was used to profile phytoplankton throughout the water columns of east and west lobes Lake Bonney to quantify the vertical structure of four functional algal groups (green algae, brown/mixed algae, cryptophytes, cyanobacteria). Our findings showed that phytoplankton communities were differentially impacted by physical and chemical factors over long-term vs. seasonal time scales. Following a summer of rapid lake level rise (2010-11), an increase in depth integrated chlorophyll a (chl-a) occurred in Lake Bonney caused by stimulation of photoautotrophic green algae. Collectively our data reveal that phytoplankton groups possessing variable trophic abilities are differentially competitive during seasonal and long-term time scales owing to periods of higher nutrients (photoautotrophs) vs. light/energy limitation (mixotrophs).

lakes phytoplankton primary production LTER Controlled Vocabulary algae Antarctica lake LTER McMurdo Dry Valleys phytoplankton primary production primary productivity Taylor Valley Station Keywords organic matter population dynamics LTER Core Areas

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https://mcm.lternet.edu/content/phytoplankton-water-column-profiles-lake-bonney-antarctica-2004-2015 Lake Bonney is a saline lake with permanent ice cover at the western end of Taylor Valley in the McMurdo Dry Valleys of Victoria Land, Antarctica. It is 7 kilometres or 4.3 mi long and up to 900 metres or 3,000 ft wide. A narrow channel only 50 metres or 160 ft wide. Lake Bonney at Narrows separates the lake into East Lake Bonney 3.32 square kilometres or 1.28 sq mi and West Lake Bonney, 0.99 square kilometres or 0.38 sq mi. The west lobe is flanked by Taylor glacier. Valley: Taylor Distance to Sea : 25 Maximum Length (km): 4.8 Maximum Width (km): 0.9 Maximum Depth (m): 37 Surface Area (km^2): 3.32 Ice Thickness Average Surface (m): 3 - 4.5 Volume (m^3 * 10^6): 54.7 162.353210449219 162.536209106445 -77.697700500488 -77.724441528320 57 57 meter Lake Bonney is a saline lake with permanent ice cover at the western end of Taylor Valley in the McMurdo Dry Valleys of Victoria Land, Antarctica. It is 7 kilometres or 4.3 mi long and up to 900 metres or 3,000 ft wide. A narrow channel only 50 metres or 160 ft wide. Lake Bonney at Narrows separates the lake into East Lake Bonney 3.32 square kilometres or 1.28 sq mi and West Lake Bonney, 0.99 square kilometres or 0.38 sq mi. Valley: Taylor Distance to Sea : 28 Maximum Length (km): 2.6 Maximum Width (km): 0.9 Maximum Depth (m): 40 Surface Area (km^2): 0.99 Ice Thickness Average Surface (m): 2.8-4.5 Volume (m^3 * 10^6): 10.1 162.269104003906 162.354934692383 -77.714805603027 -77.727287292480 57 57 meter 2004-12-13 2015-01-16 McMurdo Dry Valleys LTER Information Manager im@mcmlter.org McMurdo Dry Valleys LTER http://mcmlter.org/ McMurdo Dry Valleys LTER

A submersible spectrofluorometer (bbe Moldaenke Fluoroprobe; Beutler et al., 2002; Li et al., 2019) was lowered through the water columns in Lake Bonney beginning in the 2004-2005 field season. This instrument employs five light emitting diodes (LEDs) calibrated to the known excitation spectra of light harvesting antennae pigments of the following specific algal groups: 470 nm = green algae; 525 = brown/mixed algae; 570 nm = cryptophytes; 610 nm = cyanobacteria. An additional LED excited at 370 nm to estimate colored dissolved organic matter (CDOM), which was used to correct chl-a fluorescence.  The Fluoroprobe collected data continuously as it was lowered through the water column at approximately 20 cm s-1 yielding measurements at ~10 cm depth intervals.

LIMNO_PHYTO_PROFILE LIMNO_PHYTO_PROFILE.csv 51058 1 column , https://mcm.lternet.edu/sites/default/files/data/LIMNO_PHYTO_PROFILE.csv DATASET_CODE Dataset code Internal dataset code. string Internal dataset code. SAMPLE_ID Sample ID Sample identification code string Sample identification code LOCATION_NAME Location Location of sample string Location of sample DATE_TIME Date Date sample was collected date MM/DD/YYYY DEPTH Depth Depth sample was taken. meter real CYANOBACTERIA Cyanobacteria Calculated concentration of Cyanobacteria at fluorescence value of 610 nm, measured as Chlorophyll-a. microGramsPerLiter real CRYPTOPHYTES Cryptophytes Calculated concentration of Cryptophytes at fluorescence value of 570 nm, measured as Chlorophyll-a. microGramsPerLiter real BROWN_MIXED_ALGAE Brown/Mixed Algae Calculated concentration of Brown/Mixed Algae at fluorescence value of 525 nm, measured as Chlorophyll-a. microGramsPerLiter real GREEN_ALGAE Green Algae Calculated concentration of Green Algae at fluorescence value of 470 nm, measured as Chlorophyll-a. microGramsPerLiter real