uid=MCM,o=EDI,dc=edirepository,dc=org all public read MMNDVI Sarah Power snpower@vt.edu https://orcid.org/0000-0002-4863-290X Mark Salvatore mark.salvatore@nau.edu http://msalvatore.net https://orcid.org/0000-0002-1551-8342 Eric Sokol sokole@gmail.com http://sokole.blogspot.com/ https://orcid.org/0000-0001-5952-0917 Lee Stanish lstanish@gmail.com https://orcid.org/0000-0002-9775-6861 John "Jeb" Barrett jebarre@vt.edu https://www.biol.vt.edu/faculty/barrett/index.html https://orcid.org/0000-0002-7610-0505 McMurdo Dry Valleys LTER http://mcmlter.org/ Renée Brown rfbrown@unm.edu https://orcid.org/0000-0002-4986-7663 data manager 2020-09-09 English

This package contains data collected from microbial mat surveys (i.e., percent cover, ash-free dry mass (AFDM), and pigment concentrations – chlorophyll-a, scytonemin, and carotenoids) associated with satellite-derived Normalized Difference Vegetation Index (NDVI) values from the Lake Fryxell Basin of Taylor Valley, located in the McMurdo Dry Valleys of Antarctica. The purpose of this study was to quantitatively compare key microbial mat characteristics to NDVI. Data were collected at seven plot locations within the Canada Glacier Antarctic Specially Protected Area (ASPA) near Canada Stream, as well as alongside Green Creek and McKnight Creek. NDVI values were derived from a WorldView-2 multispectral satellite image taken of the Lake Fryxell Basin on January 19, 2018, while biological ground surveying and sampling were conducted during the 2nd and 4th weeks of January 2018. 

aboveground biomass biomass chlorophyll chlorophyll a microbial biomass primary production remote sensing satellite imagery LTER Controlled Vocabulary biomass chlorophyll LTER McMurdo Dry Valleys microbial biomass microbial mat primary production primary productivity Taylor Valley Station Keywords primary production LTER Core Areas

This data package is released under the Creative Commons Attribution 4.0 International License (CC BY 4.0; http://creativecommons.org/licenses/by/4.0/), which allows consumers (hereinafter referred to as “Data Users”) to freely reuse, redistribute, transform, or build on this work (even commercially) so long as appropriate credit is provided. Accordingly, Data Users are required to properly cite this data package in any publications or in the metadata of any derived products that result from its use (in whole or in part). A recommended citation is provided on the summary metadata page associated with this data package in the McMurdo Dry Valleys LTER Data Catalog (https://mcmlter.org/data), and a generic citation may be found on the summary metadata page in the repository where this data package was obtained. When these data contribute significantly to the contents of a publication, Data Users must also acknowledge that data were provided by the NSF-supported McMurdo Dry Valleys Long Term Ecological Research program (OPP-2224760). This data package has been released in the spirit of open scientific collaboration. Hence, Data Users are strongly encouraged to consider consultation, collaboration, and/or co-authorship (as appropriate) with the data package creator(s). Data Users should be aware these data may be actively used by others for ongoing research; thus, coordination may be necessary to prevent duplicate publication. Data Users should also recognize that misinterpretation of data may occur if they are used outside the context of the original study. Hence, Data Users are urged to contact the data package creator(s) if they have any questions regarding methodology or results. While substantial efforts are made to ensure the accuracy of this data package (with all its components), complete accuracy cannot be guaranteed. Periodic updates to this data package may occur, and it is the responsibility of Data Users to check for new versions. This data package is made available “as is” and comes with no warranty of accuracy or fitness for use. The creator(s) of this data package and the repository where these data were obtained shall not be liable for any damages resulting from misinterpretation, use, or misuse of these data. Finally, as a professional courtesy, we kindly request Data Users notify the primary contact referenced in the metadata when these data are used in the production of any derivative work or publication. Notification should include an explanation of how the data were used, along with a digital copy of the derived product(s). Thank you.

https://mcm.lternet.edu/content/microbial-mat-biomass-and-normalized-difference-vegetation-index-ndvi-values-lake-fryxell Coordinates taken from 1996-97 GPS measurements at center of creek where it enters Lake Fryxell Parent Stream: Canada Stream Provenance : GPS96-97.DOC ID: canada_mouth 163.074447600000 163.074447600000 -77.614922000000 -77.614922000000 18 18 meter Green Creek at mouth Description: Coordinates taken from 1996-97 GPS measurements at center of creek where it enters Lake Fryxell ID: green_mouth File Name :GPS96-97.DOC 163.063507080078 163.063507080078 -77.622589111328 -77.622589111328 19 19 meter McKnight Creek at mouth ID: mcknight_mouth 163.264724700000 163.264724700000 -77.599724000000 -77.599724000000 2018-01-09 2018-01-25 McMurdo Dry Valleys LTER Information Manager im@mcmlter.org McMurdo Dry Valleys LTER http://mcmlter.org/ McMurdo Dry Valleys LTER

Locations of visually conspicuous microbial mats outside of stream channels were selected for seven 100 m2 plots beside Canada Stream in the Canada Glacier Antarctic Specially Protected Area (ASPA), Green Creek, and McKnight Creek in the Lake Fryxell Basin. Plots Canada Stream-1, -2, -3, and -4 were all located in the lower flush of the ASPA and Canada Stream-5 was in the upper flush. Hemispherical photos of ground cover along with position data were taken every 5 m at each of the seven plots. Using a random stratified sampling design, we sampled microbial mat from 9 or 10 locations within each plot for pigment chemistry and ash-free dry mass (AFDM).The hemispherical photos were analyzed for microbial mat cover using ImageJ, an image analysis software. By converting the photos to binary black and white images, the software distinguished pixels representing microbial mats (black) from those representing bare soil or rocks (white). Estimates for percent cover were quantified using a histogram. Chlorophyll-a concentration was measured with a spectrophotometer (Shimadzu UV-1601) using a protocol that corrects for scytonemin concentration. The samples were dried at 105°C for 24 hrs and extracted in 10 mL of 90% unbuffered acetone using 0.5 g of mat, based on protocols from Garcia‐Pichel and Castenholz (1991) and standard methods employed by the McMurdo Dry Valleys Long Term Ecological Research program (MCM LTER). The samples were then left for 24 hrs in a dark room at ambient temperature for complete chlorophyll extraction. After centrifuging for 10 min at 4000 RPM, they were analyzed on a spectrophotometer using 10 mL cuvettes. The absorbances contributed by chlorophyll-a, carotenoids, and scytonemin were quantified at 663, 490, and 384 nm, respectively, using the trichromatic equations outlined in Garcia‐Pichel and Castenholz (1991). One sample from Canada Stream-2 had extremely high pigment concentrations overall, with values greater than 6 standard deviations of the mean chlorophyll-a concentrations for all observations, and it was removed from the dataset. AFDM was calculated by weighing approximately 0.25 g of oven-dried sample, combusting at 550°C for 60 min using a muffle furnace, and reweighing after cooling in a desiccator. Given the extremely low clay content of soils in this region, the rehydration of clays was assumed negligible, so the methods did not include rewetting samples to account for the hydration of clays.The WorldView-2 multispectral satellite (DigitalGlobe, Inc.) collected a cloud-free image of the Lake Fryxell Basin on January 19, 2018, within 10 days of our surveying and sampling. The image was georeferenced using ground control points and processed to atmospherically corrected surface reflectance using the Environment for Visualizing Images software (ENVI, Harris Geospatial). We calculated the normalized difference vegetation index (NDVI) for the image. Using ArcMap, we converted the NDVI image raster file to points and then selected the pixels whose centroids fell within the 100 m2 plot boundaries. Each plot corresponds to approximately 14-17 pixels (5.95 m2 per pixel) with unique NDVI values.

MMNDVI_CHEMISTRY Microbial mat survey chemistry MMNDVI_CHEMISTRY.csv 5737 1 column , " https://mcm.lternet.edu/sites/default/files/data/MMNDVI_CHEMISTRY.csv DATASET_CODE Dataset code Internal dataset code. string Internal dataset code. PLOT Plot name Plot location name where sample was collected. string Plot location name where sample was collected. REPLICATE Replicate number Replicate number within the associated plot. string Replicate number within the associated plot. SCYTO Scytonemin concentration Scytonemin concentration, expressed in micrograms per gram of dry soil. microgramsPerGram real NA See comments. CARO Carotenoid concentration Carotenoid concentration, expressed in micrograms per gram of dry soil. microgramsPerGram real NA See comments. CHLA Chlorophyll-a concentration Chlorophyll-a concentration, expressed in micrograms per gram of dry soil. microgramsPerGram real NA See comments. AFDM Ash Free Dry Mass Ash-free dry mass content, expressed in milligrams per gram of dry soil. milligramsPerGram real NA See comments. COMMENTS Comments Comments regarding NA values. string Comments regarding NA values. MMNDVI_COVER Microbial mat survey percent cover MMNDVI_COVER.csv 2423 1 column , " https://mcm.lternet.edu/sites/default/files/data/MMNDVI_COVER.csv DATASET_CODE Dataset code Internal dataset code. string Internal dataset code. PLOT Plot name Plot location name where sample was collected. string Plot location name where sample was collected. COVER Percent cover Percent cover value. dimensionless real MMNDVI_NDVI NDVI values MMNDVI_NDVI.csv 4108 1 column , " https://mcm.lternet.edu/sites/default/files/data/MMNDVI_NDVI.csv DATASET_CODE Dataset code Internal dataset code. string Internal dataset code. PLOT Plot location Plot location name where sample was collected. string Plot location name where sample was collected. NDVI Normalized Difference Vegetation Index Normalized Difference Vegetation Index (NDVI) values. dimensionless real MMNDVI_LOCATIONS Plot locations MMNDVI_LOCATIONS.csv 436 1 column , " https://mcm.lternet.edu/sites/default/files/data/MMNDVI_LOCATIONS.csv DATASET_CODE Dataset code Internal dataset code. string Internal dataset code. PLOT Plot location Plot location name where sample was collected. string Plot location name where sample was collected. LATITUDE Latitude Latitude of plot location. decimalDegrees real LONGITUDE Longitude Longitude of plot location. decimalDegrees real