uid=MCM,o=EDI,dc=edirepository,dc=org all public read KOHLER_PDIATOM Tyler Kohler tyler.j.kohler@gmail.com https://orcid.org/0000-0001-5137-4844 Aneliya Cox asakaeva@gmail.com Joshua Darling joshua.darling@colorado.edu https://orcid.org/0000-0001-9074-4617 Adrian Howkins

13-15 Woodland Road Bristol BS8 1TH GB

adrian.howkins@bristol.ac.uk https://orcid.org/0000-0002-9615-2337 Kateřina Kopalová k.kopalova@hotmail.com https://orcid.org/0000-0002-7905-4268 Eric Sokol sokole@gmail.com http://sokole.blogspot.com/ https://orcid.org/0000-0001-5952-0917 Michael Gooseff michael.gooseff@colorado.edu http://goosefflab.weebly.com https://orcid.org/0000-0003-4322-8315 Diane McKnight diane.mcknight@colorado.edu https://orcid.org/0000-0002-4171-1533 McMurdo Dry Valleys LTER http://mcmlter.org/ Renée Brown rfbrown@unm.edu https://orcid.org/0000-0002-4986-7663 data manager 2021-04-08 English

We performed a field survey to characterize diatom communities living in the benthic microbial mats of ponds across the McMurdo Sound region of Antarctica. Samples were collected during the Austral summers of 2012-13, 2013-14, and 2015-16 from the McMurdo Dry Valleys, Cape Royds, and from Hut Ridge near McMurdo Station. We also characterized pond diatom communities in samples collected on various dates from 1908-1909 during Ernest Shackleton’s Nimrod Expedition to compare against modern samples. Historical samples were taken from Cape Royds and the Stranded Moraines, and were analyzed from slides stored at the Natural History Museum, London. This data package includes relative abundance data for diatom species found in these modern and historical samples.

abundance communities diatoms ponds relative abundance species abundance LTER Controlled Vocabulary abundance Antarctica LTER McMurdo Dry Valleys microbial mat Station Keywords population dynamics LTER Core Areas The modern (21st century) samples in the dataset have been previously published in Sakaeva et al. (2016) and Verleyen et al. (2021). Sakaeva A., E.R. Sokol, T.J. Kohler, L.F. Stanish, S.A. Spaulding, A. Howkins, K.A. Welch, W.B. Lyons, J.E. Barrett, and D.M. McKnight. (2016). Evidence for dispersal and habitat controls on pond diatom communities from the McMurdo Sound Region of Antarctica. Polar Biology 39(12): 2441-2456. doi: 10.1007/s00300-016-1901-6 (erratum doi: 10.1007/s00300-016-1944-8) Verleyen, E., B. Van de Vijver, B. Tytgat, E. Pinseel, D.A. Hodgson, K. Kopalová, S.L. Chown, E. Van Ranst, S. Imura, S. Kudoh, W. Van Nieuwenhuyze, ANTDIAT consortium, K. Sabbe, and W. Vyverman. (In press). Diatoms define a novel freshwater biogeography of the Antarctic. Ecography 44. doi: 10.1111/ecog.05374 Funding for this work was provided by the National Science Foundation Grant #OPP-1637708 for Long Term Ecological Research.

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https://mcm.lternet.edu/content/pond-diatom-communities-mcmurdo-sound-region-antarctica-assessed-modern-samples-2012-2016 The Blue Lake North Lobe is in the Cape Roys area, In Ross Island.Code: BLNLNorth lobe of ice-covered  lake, <5 m  moat, area is about 41,529, at the time, the water temperature was about 6.3 degrees celsius. 166.176635742188 166.176635742188 -77.543998718262 -77.543998718262 The Blue Lake South Lobe is in the Cape Roys area, In Ross Island.Code: BLSLSouth lobe of ice-covered  lake, <1 m  moat, area is about 56,794 at the time, the water temperature was about 1.5 degrees celsius. 166.181732177734 166.181732177734 -77.545921325684 -77.545921325684 The Cape Royds Pond 1 is an ice free pond in the Ross IslandCode : CR1Area: 1,792 Temperature: 6.3 celsius  166.163772583008 166.163772583008 -77.552818298340 -77.552818298340 The Cape Roys Pond 2 is an ice-free pond at ROss Ilsand.Area 26Temperature: 6.2 celsiusCode: CRP2  166.177307128906 166.177307128906 -77.549003601074 -77.549003601074 The Cape Roys Pond 3 is in ROss Island, and is a predominantly ice free pond.Code: CRP3Area: 323Temperature: 5.4 celsius 166.172653198242 166.172653198242 -77.544097900391 -77.544097900391 The Cape Royds Pond 4 is located in Ross Island, predominantly ice-free with a snowbank.Code: CRP4Area 1692Temperature: 8 celsius 166.166946411133 166.166946411133 -77.543350219727 -77.543350219727 The Cape Royds Pond 5 is a small ice free pond in Ross IslandArea: 5Temperature : 9.1 166.158432006836 166.158432006836 -77.552848815918 -77.552848815918 The Cape Royds Pond 6 is a small ice-free pond at ROss Island.Code: CRP6Area: 897 Temperature: 8.9 celsius 166.172073364258 166.172073364258 -77.546722412109 -77.546722412109 The Clear lake is an Ice-covered lake with ~33 % ice-free, in the Cape Roys area in Ross Island. The area the lake covers is about 10,929Code: CLLArea: 10,929 Measured Temperature: 2.9 166.161422729492 166.161422729492 -77.542182922363 -77.542182922363 The Coast Lake is a predominantly ice free lake in the Cape Roys area in Ross Island.Code : COLLake Area: 14,813 Temperature: 10.1   166.150375366211 166.150375366211 -77.542266845703 -77.542266845703 The Green Lake is an ice-free pond, near coast Area: 3,020 Temperature : 9.4 Celsius 166.157424926758 166.157424926758 -77.549766540527 -77.549766540527 The Kaki Pond 3 is an ce-covered pond with a moat less than a meter. It is in the Taylor Bonney, looks like the Bonney Basin.Code: MP3Area:5,318Temperature:  162.711425781250 162.711425781250 -77.700569152832 -77.700569152832 The Kaki Pond 4 is located in the uplands of the Boney Basin, in the Taylor Valley, is ice covered with a moat less than a meter.Code: MP4Area:14,012 Temperature: 2.4 celsius   162.716186523438 162.716186523438 -77.696022033691 -77.696022033691 The Labyrinth Pond 4 is located in the Wright Valley. Ice-covered, heavily shaded, lense-shaped.Code: LAB4Area: 25,805Temperature: 2.6 celsius  161.063613891602 161.063613891602 -77.560478210449 -77.560478210449 A pond, 0.3 nautical miles (0.6 km) long, located 0.5 nautical miles (1 km) south of the snout of Commonwealth Glacier in Taylor Valley, Victoria Land, Antarctica. The pond is part of the Aiken Creek system and receives drainage from several glaciers including Commonwealth Glacier, Wales Glacier and the unnamed glacier next westward. 163.313079833984 163.328353881836 -77.597686767578 -77.598609924316 20 20 meter The McMurdo Hut Ridge Lower Pond is on Ross Island, small Ice-free, lense-shaped pond.Code: MCMLArea: 5Temperature: 8.6 166.644607543945 166.644607543945 -77.843482971191 -77.843482971191 The McMurdo Hut Ridge Upper Pond is an Ice-free, lense-shaped pond on Ross IslandArea: 393 Temperature: 6.3 celsius  166.645034790039 166.645034790039 -77.841217041016 -77.841217041016 The Nussbaum Riegel Pond is in the Taylor Valley in between the Bonney and Chad Basins.  An Ice-covered, oval pond.Code: NRP Area: 6,133 Temperature: 3.1 celsius 162.814727783203 162.814727783203 -77.684379577637 -77.684379577637 The Parera Pond is an Ice-covered, <1 m moat in the Taylor ValleyCode: PPArea:39,916 Temperature: 5.7 celsius 162.913177490234 162.913177490234 -77.654182434082 -77.654182434082 The Picture Pond is a former channel, oval pond in the Taylor Valley, near Explorer's Cove and Commonwealth GlacierCode PIPArea: 8,119 Temperature: 7.1 163.423706054688 163.423706054688 -77.588279724121 -77.588279724121 The Pony Lake is an Ice-covered with moat, circular pond near Cape Royds in Ross IslandCode : POLArea: 9,974Temperature 4 celsius 166.167205810547 166.167205810547 -77.552848815918 -77.552848815918 The Spaulding Pond is Mostly ice-free, circular and near Howard glacier in Taylor ValleyCode: SPP Area: 68,329 Temperature 9.9 celsius 163.123352050781 163.123352050781 -77.658096313477 -77.658096313477 1908-01-29 2016-01-04 McMurdo Dry Valleys LTER Information Manager im@mcmlter.org McMurdo Dry Valleys LTER http://mcmlter.org/ McMurdo Dry Valleys LTER

We sampled pond microbial mats from the McMurdo Dry Valleys and Ross Island, Antarctica, using a brass cork borer. Samples were preserved in ~5% formalin (final concentration), and shipped to the University of Colorado for analysis. Samples were digested using heat and hydrogen peroxide (H2O2) and rinsed several times with MilliQ water until a neutral pH was achieved. Digested material was dried onto cover slips and permanently mounted on glass microscope slides with Zrax® (W. P. Dailey, University of Pennsylvania) mounting medium and analyzed by light microscope at 1250x magnification. We also analyzed 14 of the original West &amp; West (1911) microscope slides prepared from material collected during Shackelton’s Nimrod expedition, and are stored at The Natural History Museum, London. Because of the preparation method used in making the historical slides (some had thick coverslips), several of these historical slides were counted at 650 or 850x (eg. Green Lake, Lake West side of McMurdo). For both modern and historical material, at least 300 valves were counted from each slide, and relative abundance of species were calculated (slides were also scanned afterward for rare species). Scanning electron microscopy (SEM) images were made from some contemporary samples at the Charles University in Prague after being air-dried onto aluminum stubs, which were then sputter-coated with 50 nm of Au and photographed on a JEOL JSM-6380LV at 25 kV. Diatom taxonomic identifications were performed according to the species list in Sakaeva et al. (2016), the Antarctic Freshwater Diatoms database, along with the original West and West (1911) publication. Sakaeva, A., Sokol, E.R., Kohler, T.J., Stanish, L.F., Spaulding, S.A., Howkins, A., Welch, K.A., Lyons, W.B., Barrett, J.E. and McKnight, D.M. 2016. Evidence for dispersal and habitat controls on pond diatom communities from the McMurdo Sound Region of Antarctica. Polar Biology 39(12): 2441-2456. doi: 10.1007/s00300-016-1901-6 West, W. and West, G.S. 1911. Freshwater algae. British Antarctic Expedition (1907–1909) Science Report, Biology 1: 263-298.

KOHLER_PDIATOM mcmlter-strm-kohler-pdiatom-20210408.csv 337323 1 1 column , " https://mcm.lternet.edu/sites/default/files/data/mcmlter-strm-kohler-pdiatom-20210408.csv DATASET_CODE Dataset code Internal dataset code. string Internal dataset code. SAMPLE_ID Sample ID Identification code for the specific sample string Identification code for the specific sample REGION Region Indicates the broad geographic location that the sample originated from. Categories include the McMurdo Dry Valleys, Cape Royds, McMurdo Station, and the Stranded Moraines. string Indicates the broad geographic location that the sample originated from. Categories include the McMurdo Dry Valleys, Cape Royds, McMurdo Station, and the Stranded Moraines. SITE Site Name of the waterbody that the sample originated from string Name of the waterbody that the sample originated from SITE_DESCRIPTION Site description Description of the sample site including the pond name and other details string Description of the sample site including the pond name and other details PERIOD Period Categorical value indicating whether the sample is modern or historical. string Categorical value indicating whether the sample is modern or historical. TIMESPAN Timespan The years (in the case of historical samples) or austral summer seasons (in the case of modern samples) in which the sample was collected. string The years (in the case of historical samples) or austral summer seasons (in the case of modern samples) in which the sample was collected. SAMPLE_DATE Sample Date Date samples were taken. Exact dates are given for modern samples only. date mm/dd/yyyy SPECIES Species Genus and species of the diatom. string Genus and species of the diatom. ABUNDANCE Relative abundance Percent relative abundance for each species in the sample. percent real ratio of two quantities as percent composition (1:100)