Abigail C. JacksonDiana H. WallByron J. Adams 2023-04-11 tabular digitial data McMurdo Dry Valleys LTER McMurdo Dry Valleys LTER 10.6073/pasta/45337fb617c40312ea55ea97133a4c5e https://mcm.lternet.edu/content/mitochondrial-coxi-sequences-and-associated-metazoan-abundances-soils-collected-mcmurdo-dry As part of an ongoing long-term sampling effort conducted by the McMurdo Dry Valleys Long Term Ecological Research (LTER) project, the top 10 cm of soil was collected from sampling sites across the McMurdo Dry Valleys region of Antarctica. A subset of these samples representing each valley and a differing disturbance legacy from the last glacial maximum were analyzed for this data package. Samples were collected between 1995 and 2022. In each sample, the abundances of three genera of nematodes (Scottnema, Eudorylaimus, and Plectus), tardigrades, and rotifers were calculated. Following metazoan extractions, individual Scottnema lindsayae were selected for mitochondrial Cytochrome c oxidase I (COX1) Sanger dideoxy sequencing. Sequencing resulted in 249 assembled sequences of 285 base pairs after alignment and trimming. Funding: Provided by several grants from the National Science Foundation for Long Term Ecological Research, most recently #OPP-1637708.Provenance: Metazoan abundance data were derived from the MCM LTER Biotic Effects (10.6073/pasta/41825ac3c486a2858c51462e968e3a42) and Elevational Transect (10.6073/pasta/6c9dea12fe86439c0d390726348b0d5f) experiments. 1995-12-01 2022-02-28 ground condition As needed This is the Taylor Valley, a subjectively approximated contour by San Gil. We use this to denote the geographical extent of studies that encompass the whole Taylor Valley.According to Wiki contributors, the Taylor Valley is the southernmost of the three large Dry Valleys in the Transantarctic Mountains, Victoria Land, located west of McMurdo Sound. The valley extends from Taylor Glacier in the west to McMurdo Sound at Explorers Cove at the northwest head of New Harbour in the east and is about 29 kilometres (18 mi) long. It was once occupied by the receding Taylor Glacier, from which it derives its name. Taylor Valley contains Lake Bonney in the west (inward), and Lake Fryxell in the east (coastward), and Lake Hoare, Lake Chad, Mummy Pond and Parera Pond close together between the two. Further east of Lake Bonney is Pearse Valley. Taylor Valley is separated from Wright Valley in the north by Asgard Range, and from Ferrar Glacier in the south by Kukri Hills. 163.624877929690 161.707763671880 -77.519802097166 -77.808487073526 LTER Core Areas population dynamics None <cntperp> <cntper>McMurdo Dry Valleys LTER Information Manager</cntper> </cntperp> <cntemail>im@mcmlter.org</cntemail> Name: Renée F. Brown Role: data manager Not Applicable Not Applicable Field and/or Lab Methods About 500 g of the top 10 cm of soil were collected with clean plastic scoops and sterile Whirlpak bags from sites in the MDVs during the Austral summer between 1996 to 2022. Soils were transported to the Crary Laboratory in McMurdo Station where they were stored at 4 °C until soil invertebrates and geochemistry could be performed within 48 hours. A sugar centrifugation extraction (Freckman and Virginia, 1993) was performed with a subsample of 100 g to calculate metazoan abundance. Soil invertebrates were counted and identified to species, as living or dead, life stage (juvenile or adults), and sex. Soil moisture, pH, and electrical conductivity were measured following standard protocols of the MCM LTER project (e.g., http://mcm.lternet.edu/content/soil-elevational-transect-experiment). Subsequent subsamples of 100 g were extracted in the same way at Brigham Young University to collect individual Scottnema lindsayae. Following Powers and Harris (1993) nematode extraction methods, individual S. lindsayae (Timm, 1971) were hand-picked with an eyelash tool onto 10 μl of elution buffer on a cover slip. Using a clear, sterilized 10 μl micropipette tip, the nematode was ruptured by gentle pressure and lysate confirmed in the sterile water. Lysate was moved to a 0.2 ml microcentrifuge tube. The cover slip was further cleaned with 5.2 μl of sterile water, which was added to the microcentrifuge tube. Nematode lysate was combined with 2.4 μl each of 20 μM diluted forward primer (COI-Scott-F1: 5'-GTTACAACTTTTTTGCTTATTCTCTCAC-3’) and reverse primer (COI-Scott-R2: 5’-CTGTAAAATAAGCTCGACTRTCWG-3’).  Additional extractions were performed with an alternative reverse primer with higher fidelity, but shorter sequence reads (COI-Scott-R4 5’-GCRTCRATACCTGTTACRTATATRTG-3’). Additionally, 15 μl of JumpStart™ REDTaq® ReadyMix™ Reaction Mix (Sigma-Aldrich, DE) was added to the mixture and vortexed for 30 seconds to mix. The mixture was placed in a DNA thermal cycler (Mastercycler X50s, Eppendorf, Germany) on the following settings: initial denaturation at 94°C for 5 minute, 40 cycles of denaturation at 94°C for 30 seconds, annealing at 52°C for 30 seconds, extension of 0.5°C/second ramp to 72 for 90 seconds. Followed by a final extension at 72°C for 5 minutes and hold at 4°C. Following PCR amplification, the products were screened on a 1% TAE agarose gel stained with ethidium bromide and visualized with a UV image capture (GelDoc Go, Bio-Rad Labratories, USA). Successful DNA amplifications were cleaned with a magnetic bead cleaning protocol. Mag-Bind® TotalPure NGS (Omega Bio-tek, GA) was added in a 1.2x concentration to the remaining PCR product, vortexed for 10 minutes at 2,000 rpm, centrifuged for 5 seconds, placed on a magnetic rack until bead solution was cleared. The supernatant was removed and thrown away, while the beads were washed with 200 μl 80% ethanol for 30 seconds twice. After removing all ethanol, residual ethanol was allowed to evaporate for 10 minutes.  Tubes were removed from the magnetic rack as 30 ml of elution buffer (Omega Bio-tek, GA) was added and vortexed to mix. Tubes were returned to the magnetic rack to separate the supernatant. The supernatant was removed and added to a clean 0.2 ml tube and beads discarded. DNA concentration was quantified by Quibit 4 Flurorometer (Invitrogen, USA). DNA was diluted or concentrated to 10 ng for Sanger dideoxy sequencing at the Brigham Young University Sequencing Center (Provo, UT). Analysis was conducted with a combination of ARLEQUIN v3.5.2.2 (Excoffier and Lischer., 2010), R Studio (R Core Team, 2002) utilizing pegas (Paradis, 2010), haplotypes (Aktas, 2020), poppr 2.9.3 (Kamvar, Tabima and Grunwald, 2014; Kamvar, Brooks, Grunwald, 2015), and adegenet (Jombart, 2008; Jombar and Ahmed 2011) packages. Phylogeographic reconstructions were recreated in IQ-tree with the built-in model finder and ultrafast bootstrap values (Kalyaanamoorthy et al., 2017; Hoang et al., 2018; Minh et al., 2020) and FigTree v1.4.4 (available at http://tree.bio.ed.ac.uk/software/figtree/). Bayesian Skyline plots were computed with BEAST v2.6.7 (Bouckaert et al., 2019) and tracer. References Cited: Aktas, C. (2020) “haplotypes: Manipulating DNA Sequences and Estimating Unambiguous Haplotype Network with Statistical Parsimony.” Bouckaert, R. et al. (2019) “BEAST 2.5: An advanced software platform for Bayesian evolutionary analysis.” PLoS computational biology. doi: e1006650. Excoffier, L. and Lischer, H. E. L. (2010) “Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows.” Molecular Ecology Resources, pp. 10:564-567. Freckman, D. W. and Virginia, R. A. (1993) “Extraction of nematodes from Dry Valley Antarctic soils,” Polar Biology, 13(483–487). doi: https://doi.org/10.1007/BF00233139. Hoang, D. T. et al. (2018) “UFBoot2: Improving the ultrafast bootstrap approximation.” Molecular Biology and Evolution, pp. 35:518-522. Jombart, T. (2008) “adegenet: a R package for the multivariate analysis of genetic markers.” Bioinformatics, pp. 24:1403-1405. doi: 10.1093/bioinformatics/btn129. Jombart, T. and Ahmed, I. (2011) “adegenet 1.3-1: new tools for the analysis of genome-wide SNP data.” Bioinformatics. doi: 10.1093/bioinformatics/btr521. Kalyaanamoorthy, S. et al. (2017) “ModelFinder: Fast model selection for accurate phylogenetic estimates.” Nat. Methods, pp. 14:587-589. Available at: https://doi.org/10.1038/nmeth.4285. Kamvar, Z. N., Brooks, J. C. and Grunwald, N. J. (2015) “Novel R tools for analysis of genome-wide population genetic data with emphasis on clonality.” Frontiers in Genetics, p. 6:208. doi: 10.3389/fgene.2015.00208. Kamvar, Z. N., Tabima, J. . and Grunwald, N. J. (2014) “Poppr: an R package for genetic analysis of populations with clonal, partially clonal, and/or sexual reproduction.” PeerJ. doi: 10.7717/peerj.281. Minh, B. Q. et al. (2020) “IQ-TREE 2: New models and efficient methods for phylogenetic inference in the genomic era.” Molecular Biology and Evolution, pp. 37: 1530-1534. Paradis, E. (2010) “pegas: an R package for population genetics with an integrated-modular approach.” Bioinformatics, pp. 26: 419-420. Powers, T. O. and Harris, T. S. (1993) “A polymerase chain reaction method for identification of five major meloidogyne species.,” Journal of nematology, 25(1), pp. 1–6. Available at: http://www.ncbi.nlm.nih.gov/pubmed/19279734%0Ahttp://www.pubmedcentral.n.... R Core Team (2002) “R: A language and environment for statistical computing. R Foundation for Statistical Computing.” Vienna, Austria. Available at: https://www.r-project.org/. Timm, R. W. (1971) “Antarctic soil and freshwater nematodes from the McMurdo Sound Region,” Proceedings Helminth Soc Washington, 38(1), pp. 42–52. About 500 g of the top 10 cm of soil were collected with clean plastic scoops and sterile Whirlpak bags from sites in the MDVs during the Austral summer between 1996 to 2022. Soils were transported to the Crary Laboratory in McMurdo Station where they were stored at 4 °C until soil invertebrates and geochemistry could be performed within 48 hours. A sugar centrifugation extraction (Freckman and Virginia, 1993) was performed with a subsample of 100 g to calculate metazoan abundance. Soil invertebrates were counted and identified to species, as living or dead, life stage (juvenile or adults), and sex. Soil moisture, pH, and electrical conductivity were measured following standard protocols of the MCM LTER project (e.g., http://mcm.lternet.edu/content/soil-elevational-transect-experiment). Subsequent subsamples of 100 g were extracted in the same way at Brigham Young University to collect individual Scottnema lindsayae.Following Powers and Harris (1993) nematode extraction methods, individual S. lindsayae (Timm, 1971) were hand-picked with an eyelash tool onto 10 μl of elution buffer on a cover slip. Using a clear, sterilized 10 μl micropipette tip, the nematode was ruptured by gentle pressure and lysate confirmed in the sterile water. Lysate was moved to a 0.2 ml microcentrifuge tube. The cover slip was further cleaned with 5.2 μl of sterile water, which was added to the microcentrifuge tube. Nematode lysate was combined with 2.4 μl each of 20 μM diluted forward primer (COI-Scott-F1: 5'-GTTACAACTTTTTTGCTTATTCTCTCAC-3’) and reverse primer (COI-Scott-R2: 5’-CTGTAAAATAAGCTCGACTRTCWG-3’).  Additional extractions were performed with an alternative reverse primer with higher fidelity, but shorter sequence reads (COI-Scott-R4 5’-GCRTCRATACCTGTTACRTATATRTG-3’). Additionally, 15 μl of JumpStart™ REDTaq® ReadyMix™ Reaction Mix (Sigma-Aldrich, DE) was added to the mixture and vortexed for 30 seconds to mix. The mixture was placed in a DNA thermal cycler (Mastercycler X50s, Eppendorf, Germany) on the following settings: initial denaturation at 94°C for 5 minute, 40 cycles of denaturation at 94°C for 30 seconds, annealing at 52°C for 30 seconds, extension of 0.5°C/second ramp to 72 for 90 seconds. Followed by a final extension at 72°C for 5 minutes and hold at 4°C.Following PCR amplification, the products were screened on a 1% TAE agarose gel stained with ethidium bromide and visualized with a UV image capture (GelDoc Go, Bio-Rad Labratories, USA). Successful DNA amplifications were cleaned with a magnetic bead cleaning protocol. Mag-Bind® TotalPure NGS (Omega Bio-tek, GA) was added in a 1.2x concentration to the remaining PCR product, vortexed for 10 minutes at 2,000 rpm, centrifuged for 5 seconds, placed on a magnetic rack until bead solution was cleared. The supernatant was removed and thrown away, while the beads were washed with 200 μl 80% ethanol for 30 seconds twice. After removing all ethanol, residual ethanol was allowed to evaporate for 10 minutes.  Tubes were removed from the magnetic rack as 30 ml of elution buffer (Omega Bio-tek, GA) was added and vortexed to mix. Tubes were returned to the magnetic rack to separate the supernatant. The supernatant was removed and added to a clean 0.2 ml tube and beads discarded. DNA concentration was quantified by Quibit 4 Flurorometer (Invitrogen, USA). DNA was diluted or concentrated to 10 ng for Sanger dideoxy sequencing at the Brigham Young University Sequencing Center (Provo, UT).Analysis was conducted with a combination of ARLEQUIN v3.5.2.2 (Excoffier and Lischer., 2010), R Studio (R Core Team, 2002) utilizing pegas (Paradis, 2010), haplotypes (Aktas, 2020), poppr 2.9.3 (Kamvar, Tabima and Grunwald, 2014; Kamvar, Brooks, Grunwald, 2015), and adegenet (Jombart, 2008; Jombar and Ahmed 2011) packages. Phylogeographic reconstructions were recreated in IQ-tree with the built-in model finder and ultrafast bootstrap values (Kalyaanamoorthy et al., 2017; Hoang et al., 2018; Minh et al., 2020) and FigTree v1.4.4 (available at http://tree.bio.ed.ac.uk/software/figtree/). Bayesian Skyline plots were computed with BEAST v2.6.7 (Bouckaert et al., 2019) and tracer.References Cited:Aktas, C. (2020) “haplotypes: Manipulating DNA Sequences and Estimating Unambiguous Haplotype Network with Statistical Parsimony.”Bouckaert, R. et al. (2019) “BEAST 2.5: An advanced software platform for Bayesian evolutionary analysis.” PLoS computational biology. doi: e1006650.Excoffier, L. and Lischer, H. E. L. (2010) “Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows.” Molecular Ecology Resources, pp. 10:564-567.Freckman, D. W. and Virginia, R. A. (1993) “Extraction of nematodes from Dry Valley Antarctic soils,” Polar Biology, 13(483–487). doi: https://doi.org/10.1007/BF00233139.Hoang, D. T. et al. (2018) “UFBoot2: Improving the ultrafast bootstrap approximation.” Molecular Biology and Evolution, pp. 35:518-522.Jombart, T. (2008) “adegenet: a R package for the multivariate analysis of genetic markers.” Bioinformatics, pp. 24:1403-1405. doi: 10.1093/bioinformatics/btn129.Jombart, T. and Ahmed, I. (2011) “adegenet 1.3-1: new tools for the analysis of genome-wide SNP data.” Bioinformatics. doi: 10.1093/bioinformatics/btr521.Kalyaanamoorthy, S. et al. (2017) “ModelFinder: Fast model selection for accurate phylogenetic estimates.” Nat. Methods, pp. 14:587-589. Available at: https://doi.org/10.1038/nmeth.4285.Kamvar, Z. N., Brooks, J. C. and Grunwald, N. J. (2015) “Novel R tools for analysis of genome-wide population genetic data with emphasis on clonality.” Frontiers in Genetics, p. 6:208. doi: 10.3389/fgene.2015.00208.Kamvar, Z. N., Tabima, J. . and Grunwald, N. J. (2014) “Poppr: an R package for genetic analysis of populations with clonal, partially clonal, and/or sexual reproduction.” PeerJ. doi: 10.7717/peerj.281.Minh, B. Q. et al. (2020) “IQ-TREE 2: New models and efficient methods for phylogenetic inference in the genomic era.” Molecular Biology and Evolution, pp. 37: 1530-1534.Paradis, E. (2010) “pegas: an R package for population genetics with an integrated-modular approach.” Bioinformatics, pp. 26: 419-420.Powers, T. O. and Harris, T. S. (1993) “A polymerase chain reaction method for identification of five major meloidogyne species.,” Journal of nematology, 25(1), pp. 1–6. Available at: http://www.ncbi.nlm.nih.gov/pubmed/19279734%0Ahttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC2619349.R Core Team (2002) “R: A language and environment for statistical computing. R Foundation for Statistical Computing.” Vienna, Austria. Available at: https://www.r-project.org/.Timm, R. W. (1971) “Antarctic soil and freshwater nematodes from the McMurdo Sound Region,” Proceedings Helminth Soc Washington, 38(1), pp. 42–52. unknown Scottnema lindsayae mitochondrial Cytochrome c oxidase I (COI) sequences List of each individual Scottnema lindsayae sanger sequenced for a portion of the mitochondrial DNA COX1 gene and the corresponding aligned and trimmed sequences. Dataset Code Internal dataset code. The data provider Internal dataset code. Sequence ID Individual Scottnema lindsayae sequence identifier. The data provider Individual Scottnema lindsayae sequence identifier. Site Name Name of the location where soils were sampled. The data provider Name of the location where soils were sampled. Species Species identity. The data provider Species identity. Sequence Assembled sequences from a portion of the mitochondrial COX1 gene. The data provider Assembled sequences from a portion of the mitochondrial COX1 gene. NCBI Accession Number NCBI nucleotide accession number corresponding with each sequence. https://www.ncbi.nlm.nih.gov/nuccore/?term=OP456699:OP456947[accn] The data provider NCBI nucleotide accession number corresponding with each sequence. https://www.ncbi.nlm.nih.gov/nuccore/?term=OP456699:OP456947[accn] McMurdo Dry Valleys LTER The data distributor shall not be liable for innacuracies in the content http 1 0 \n 1 column , https://mcm.lternet.edu/sites/default/files/data/mcmlter-soils-jackson-soils_scott_coiseq-20230411.csv None Scottnema lindsayae LSU sequences List of each individual Scottnema sanger sequenced for 28S large subunit ribosomal ribonucleic acid (LSU) and the corresponding aligned and trimmed LSU sequence. Dataset Code Internal dataset code. The data provider Internal dataset code. Sequence ID Individual Scottnema lindsayae sequence identifier. The data provider Individual Scottnema lindsayae sequence identifier. Site Name Name of the location where soils were sampled. The data provider Name of the location where soils were sampled. Species Species identity. The data provider Species identity. Sequence Assembled sequences of a portion of the 28S large subunit ribosomal ribonucleic acid (LSU) gene. The data provider Assembled sequences of a portion of the 28S large subunit ribosomal ribonucleic acid (LSU) gene. NCBI Accession Number NCBI nucleotide accession number corresponding with each sequence. https://www.ncbi.nlm.nih.gov/nuccore/?term=OP692666:OP692695[accn] The data provider NCBI nucleotide accession number corresponding with each sequence. https://www.ncbi.nlm.nih.gov/nuccore/?term=OP692666:OP692695[accn] McMurdo Dry Valleys LTER The data distributor shall not be liable for innacuracies in the content http 1 0 \n 1 column , https://mcm.lternet.edu/sites/default/files/data/mcmlter-soils-jackson-soils_scott_lsuseq-20230411.csv None Scottnema lindsayae glacial history populations and genetic diversity Sample locations and corresponding molecular diversity metrics from each sampling population. This table also contains the nematode counts from each sampling location and sample site metadata. Dataset Code Internal dataset code. The data provider Internal dataset code. Site Name Name of the location where soils were sampled. The data provider Name of the location where soils were sampled. Valley Name The name of the dry valley each site is located in. The data provider The name of the dry valley each site is located in. Collection Year Year that soils were collected in, corresponding with the associated austral summer field season. E.g., 2019 means samples were collected over the 2018-2019 field season (i.e., between December 2018 and February 2019). The data provider calendar date/time YYYY gregorian calendar Latitude Latitude of sampling sites taken by GPS. The data provider decimalDegrees Longitude Longitude of sampling sites taken by GPS. The data provider decimalDegrees Distance to Coast A linear distance from the sample site to the coast. The data provider kilometer Last Glacial Maximum Glacial History Status of the site as a site that has either remained undisturbed or disturbed by glacial incursion or paleolake inundation during the last glacial maximum (LGM). The data provider disturbed Disturbed during the Last Glacial Maximum. The data provider undisturbed Undisturbed during the Last Glacial Maximum. The data provider Elevation Elevation of the sampling sites. The data provider meter Number of Sequences Number of individuals sanger sequenced from each sampling site. The data provider Number of individuals sanger sequenced from each sampling site. Number of Haplotypes Number of haplotypes recovered from sequenced individual nematodes from each sampling site. The data provider Number of haplotypes recovered from sequenced individual nematodes from each sampling site. Number of Polymorphic Sites Number of polymorphic sites among the haplotypes from sequenced individual nematodes from each sampling site. The data provider Number of polymorphic sites among the haplotypes from sequenced individual nematodes from each sampling site. Tajima's D Tajima’s D is a neutrality test statistic, calculated as the difference between mean number of pairwise nucleotide differences and polymorphic sites within each sampling site. The data provider Tajima’s D is a neutrality test statistic, calculated as the difference between mean number of pairwise nucleotide differences and polymorphic sites within each sampling site. Tajima's D p-value The p-value calculated from 1,000 permutations of Tajima’s D test. The data provider The p-value calculated from 1,000 permutations of Tajima’s D test. Fu’s FS Fu’s FS neutrality test statistic. The data provider Fu’s FS neutrality test statistic. Fu's FS p-value The p-value calculated from 1,000 permutations of Fu’s FS test. The data provider The p-value calculated from 1,000 permutations of Fu’s FS test. Haplotype Diversity Mean The mean probability that two randomly sampled nucleotides will differ calculated over 1,000 permutations. The data provider The mean probability that two randomly sampled nucleotides will differ calculated over 1,000 permutations. Haplotype Diversity Standard Deviation The standard deviation of haplotype diversity. The data provider The standard deviation of haplotype diversity. Nucleotide Diversity Mean The nucleotide diversity averaged over all loci calculated as the average number of nucleotide differences per site among sequences from the same sampling site over 1,000 permutations. The data provider The nucleotide diversity averaged over all loci calculated as the average number of nucleotide differences per site among sequences from the same sampling site over 1,000 permutations. Nucleotide Diversity Standard Deviation The standard deviation of nucleotide diversity. The data provider The standard deviation of nucleotide diversity. Within Population Fst Mean Mean genetic divergence within each sampling site. The data provider Mean genetic divergence within each sampling site. Within Population Fst Standard Deviation The standard deviation of within population genetic differentiation (FST). The data provider The standard deviation of within population genetic differentiation (FST). Expected Heterozygosity Mean The expected heterozygosity over all loci. The data provider The expected heterozygosity over all loci. Expected Heterozygosity Standard Deviation The standard deviation of the expected heterozygosity over all loci. The data provider The standard deviation of the expected heterozygosity over all loci. Soil Moisture Gravimetric water content of the sample as percent grams of water per gram of soil. The data provider gramsPerGram Scottnema Male Live The total number of living male Scottnema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Scottnema Male Dead The total number of dead male Scottnema adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Scottnema Female Live The total number of living female Scottnema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent The data provider 0 # kg/soil Scottnema Female Dead The total number of dead female Scottnema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent The data provider 0 # kg/soil Scottnema Juvenile Live The total number of living juvenile Scottnema nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Scottnema Juvenile Dead The total number of dead juvenile Scottnema nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Scottnema Adult The total number of living and dead Scottnema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider # kg/soil Scottnema Juvenile The total number of living and dead Scottnema lindsayae juvenile nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider # kg/soil Scottnema Total Live Total number of living Scottnema lindsayae (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Scottnema Total Dead The total number of dead Scottnema lindsayae (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Total Scottnema Live and Dead The total number of live + dead Scottnema lindsayae (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Eudorylaimus Male Live The total number of living male Eudorylaimus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent The data provider 0 # kg/soil Eudorylaimus Male Dead The total number of dead male Eudorylaimus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent The data provider 0 # kg/soil Eudorylaimus Female Live The total number of living female Eudorylaimus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent The data provider 0 # kg/soil Eudorylaimus Female Dead The total number of dead female Eudorylaimus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent The data provider 0 # kg/soil Eudorylaimus Juvenile Live The total number of living juvenile Eudorylaimus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent The data provider 0 # kg/soil Eudorylaimus Juvenile Dead The total number of dead juvenile Eudorylaimus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent The data provider 0 # kg/soil Eudorylaimus Adult The total number of living and dead Eudorylaimus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Eudorylaimus Juvenile The total number of living and dead Eudorylaimus spp. juvenile nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent The data provider 0 # kg/soil Total Eudorylaimus Live total number of living Eudorylaimus spp. (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent The data provider 0 # kg/soil Total Eudorylaimus Dead The total number of dead Eudorylaimus spp. (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent The data provider 0 # kg/soil Total Eudorylaimus Live and Dead The total number of live + dead Eudorylaimus spp. (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent The data provider 0 # kg/soil Plectus Female Live The total number of living female Plectus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent The data provider 0 # kg/soil Plectus Female Dead The total number of dead female Plectus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent The data provider 0 # kg/soil Plectus Juvenile Live The total number of living juvenile Plectus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent The data provider 0 # kg/soil Plectus Juvenile Dead The total number of dead juvenile Plectus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent The data provider 0 # kg/soil Plectus Adult The total number of living and dead Plectus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent The data provider 0 # kg/soil Plectus Juvenile The total number of living and dead Plectus spp. juvenile nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent The data provider 0 # kg/soil Total Plectus Live total number of living Plectus spp. (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent The data provider 0 # kg/soil Total Plectus Dead The total number of dead Plectus spp. (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent The data provider 0 # kg/soil Total Plectus Live and Dead The total number of live + dead Plectus spp. (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent The data provider 0 # kg/soil total rotifers total number of rotifers per kg soil oven dry weight equivalent present in a sample The data provider 0 # kg/soil Total Tardigrades Total number of tardigrades per kg soil oven dry weight equivalent present in a sample. The data provider 0 # kg/soil Total Mites Total number of mites per kg soil oven dry weight equivalent present in a sample. The data provider # kg/soil Total Live Nematodes The total number of living nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider The total number of living nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. Total Dead Nematodes The total number of dead nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider The total number of dead nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. Total Live and Dead Nematodes The total number of living and dead nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider The total number of living and dead nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. McMurdo Dry Valleys LTER The data distributor shall not be liable for innacuracies in the content http 1 0 \n 1 column , https://mcm.lternet.edu/sites/default/files/data/mcmlter-soils-jackson-soils_scott_popdiv-20230411.csv None Haplotypes Haplotypes specific to each sampling site. Dataset Code Internal dataset code. The data provider Internal dataset code. Site Name Name of the location were soils were sampled. The data provider Name of the location were soils were sampled. Haplotype ID Haplotype identifiers specific to each sampling site. The data provider Haplotype identifiers specific to each sampling site. McMurdo Dry Valleys LTER The data distributor shall not be liable for innacuracies in the content http 1 0 \n 1 column , https://mcm.lternet.edu/sites/default/files/data/mcmlter-soils-jackson-soils_scott_haplotype-20230411.csv None 2023-04-11 2023-04-11 McMurdo Dry Valleys LTER http://mcmlter.org/ Biological Data Profile of the Content Standards for Digital Geospatial Metadata devised by the Federal Geographic Data Committee. Drupal Ecological information Management Systems, version D7, Biological Data Profile module