This dataset contains a series of grab samples collected from nutrient tracer injection experiments conducted in supraglacial streams on Canada Glacier in Taylor Valley, Antarctica. The purpose of these experiments was to measure potential nutrient uptake in supraglacial stream networks. We chose three reaches and co-injected nutrient tracer(s) and a conservative tracer (NaCl). Tracers were injected at the top of each reach, allowed to mix with the stream water, and move downstream.