Surface water and hyporheic porewater samples were collected at high frequency over two periods (01/20/2018–01/21/2018 and 01/10/2019–01/12/2019) from the lower reaches of Von Guerard Stream, Taylor Valley. Porewater samples were collected using plastic tubing inserted to depths of 15 or 30 cm and drawn out by syringe. This data set shows sampling locations, water chemistry (including concentrations for dissolved organic nitrogen species, dissolved organic carbon, silica, as well as water isotopes). A laboratory nitrification potential assay was performed on sediments from Von Guerard stream to assess the functional microbial potential of the hyporheic microbial community to perform nitrification.
Porewater samplers were flexible polyethylene tubing (3/16” inner diameter) encased in a rigid plastic tube and screened at the bottom with fine metal mesh. We installed samplers to depths of both 15 and 30 cm at locations A-D on each transect. Nutrient and DOC samples were filtered through pre-combusted (4 hr at 450ºC) Whatman GF/C glass fiber filters. Nutrient samples were stored frozen in 60 mL HDPE bottles, while DOC and isotope samples were kept chilled (+4ºC) in the dark prior to analysis. Silica and DIN were measured colorimetrically immediately after thawing at room temperature by the Arikaree Environmental Laboratory, University of Colorado Boulder, using a Lachat (USA) QuikChem 8500 Flow Injection Autoanalyzer. Nitrate (as NO3– + NO2–) was analyzed by standard method 4500-NO3 I (cadmium reduction flow injection) with a detection limit of 0.004 mg NO3––N L–1. Samples were analyzed for NH4+ by standard method 4500-NH3 H (phenolate flow injection, detection limit of 0.005 mg NH4+–N L–1). For Si, the detection limit was 0.004 mg Si L–1 by standard method 4500-SiO2 F. We measured water isotopes (δ18O and δD) using a cavity ring-down spectrometer (Picarro, USA) and calculated deuterium excess as δD – 8*δ18O.For the nitrification potential assay we used sediment from two pilot transects during January 2018. We first removed the top 5 cm of the streambed to avoid contamination from benthic algal mats, then scooped sediment from approximately 5–10 cm depth into sterile Whirlpaks. These sediment samples were stored frozen and shipped to the University of Colorado Boulder for analysis. After thawing the samples at room temperature, we subsampled 20 g of wet sediment from each site in triplicate into sterile 250 mL HDPE bottles and an additional 20 g in triplicate into pre-weighed aluminum weigh boats. We dried the subsamples in the aluminum weigh boats at 105ºC for 24 hours and reweighed to calculate mean volumetric water content for each sampling location. For the samples in 250 mL HDPE bottles, we performed a nitrification potential assay using perchlorate and NH4+ in a phosphate (KH2PO4 and K2HPO4) buffered solution, which was mixed constantly with the sediment on a shaker table at room temperature. A detailed description of the assay protocol and solution concentrations is provided by Schmidt & Belser . We took subsamples from each replicate flask at 0, 1 and 8 hours then 1, 2, 3, and 5 days from the start of the assay. All subsamples were immediately filtered through Whatman GF/C glass fiber filters and stored frozen (-20ºC) until thawed for analysis. As perchlorate in the incubation solution inhibits the final oxidation of nitrite (NO2–) to NO3–, we analyzed the filtered aliquots for NO2– colorimetrically using a Lachat QuikChem 8500 Flow Injection Autoanalyzer. Accumulated NO2– concentrations were converted to mass NO2––N normalized by the calculated dry weight of sediment in each replicate flask.