Five glaciers (Taylor, Howard, Hughes, Commonwealth and Canada) were sampled for cryoconite hole invertebrates over a period of three weeks in November 2001 by Thomas Nylen, Nichole Alhadeff, and Dorota Porazinska. Exact sampling dates are described in the data file. All cryoconite holes at sampling time were entirely frozen. Each glacier (except Taylor) was sampled at four locations namely four corners of the glacier (Lower West, Lower East, Upper West, and Upper East). Upper and Lower description relates to topographical orientation of the glacier and East and West to geographical orientation. The Howard glacier was also sampled at the location described as Middle East. Because geographical and topographical orientation of the Taylor glacier overlap, samples were taken in a transect starting from the most West and Upper stake 90 and ending at stake 92 at the glacier's terminus. GPS coordinates were measured at each sampling location. Typically, 6 cryoconite holes at each location were drilled with a Sipre ice corer until the sediment would show up in the drilling dust accumulating on the ice surface. Each cryoconite hole was measured for diameter (N-S and E-W directions) and depth (from the ice surface to bottom of the sediment layer). Drilling was done by Thomas Nylen. At each location, two largest, two median, and two small cryoholes were sampled. All necessary equipment for drilling was pulled on banana sleds. Excavated ice cores (ony 20cm from the bottom of the sediment up) were placed in Nalgene plastic bags, carried in back packs to Lake Hoare camp, transported to McMurdo Cray Laboratory, and kept frozen till extraction.