In this data package, we present chlorophyll concentrations, percent loss-on-ignition organic matter, sorbed ammonium concentrations, and percent biogenic silica for hyporheic sediments collected in January 2019 from nine transects across Von Guerard Stream, Taylor Valley, Antarctica. These samples were collected to address questions about the retention and processing of particulate organic matter in the hyporheic zone of McMurdo Dry Valley streams. The nine transects were located at pools, riffles, and meanders (three of each geomorphology type) along Von Guerard Stream and extended across the stream channel to the edges of the wetted zone, ranging from 6.6 to 13.6 m in length. At each sampling location, we collected subsurface bulk sediment down to a depth of 10 cm. We analyzed the sediment samples for chlorophyll-a, phaeophytin, loss-on-ignition, ammonium sorbed to the sediment, and the UV absorbance at 254 nm of sediment extractions. This data package is associated with a complementary data package that contains diatom community assemblages for the same samples.